Overview

  • Product name

  • Description

    Rabbit polyclonal to SRBC
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human SRBC (internal sequence) conjugated to keyhole limpet haemocyanin.

  • Positive control

    • A2058 cell lysates
  • General notes

    Protein previously labeled as PRKCDBP.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Ammonium Sulphate Precipitation
  • Purification notes

    This antibody is prepared by Saturated Ammonium Sulfate precipitation followed by dialysis against PBS.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76676 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    ELISA: 1/1000.
    WB: 1/50 - 1/100. Predicted molecular weight: 28 kDa.
    Bands at 40-45 kDa may also occur due to 2 possible prenylation sites

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Plays a role in the regulation of the circadian clock. Modulates the period length and phase of circadian gene expression and also regulates expression and interaction of the core clock components PER1/2 and CRY1/2. Seems to have an immune potentiation function.
    • Tissue specificity

      Strongly expressed in mammary and epithelial cells.
    • Sequence similarities

      Belongs to the PTRF/SDPR family.
    • Post-translational
      modifications

      In vitro, phosphorylated by PRKCD.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • Cavin 3 antibody
      • Cavin-3 antibody
      • CAVIN3 antibody
      • hSRBC antibody
      • MGC20400 antibody
      • PRDBP_HUMAN antibody
      • Prkcdbp antibody
      • Protein kinase C delta binding protein antibody
      • Protein kinase C delta-binding protein antibody
      • Sdr related gene product that binds to c kinase antibody
      • Serum deprivation response factor related gene product that binds to C kinase antibody
      • Serum deprivation response factor-related gene product that binds to C-kinase antibody
      • SRBC antibody
      see all

    Images

    • Anti-SRBC antibody (ab76676) at 1/50 dilution + A2058 cell lysate at 35 µg

      Predicted band size: 28 kDa
      Observed band size: 29 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands.

    References

    This product has been referenced in:

    • Vogel ER  et al. Caveolin-1 Scaffolding Domain Peptide Prevents Hyperoxia-Induced Airway Remodeling in a Neonatal Mouse Model. Am J Physiol Lung Cell Mol Physiol N/A:N/A (2019). Read more (PubMed: 31042080) »
    • Sathish V  et al. Inflammation, caveolae and CD38-mediated calcium regulation in human airway smooth muscle. Biochim Biophys Acta 1843:346-51 (2014). Read more (PubMed: 24275509) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Lung)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    Lung
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C

    Abcam user community

    Verified customer

    Submitted Nov 11 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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