For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
ab16885 = Mouse monoclonal [Clone 327] to Src Will this antibody crossreact with other src family members e.g. fyn and lyn in IP experiments? Has this antibody been tested in mouse platelet samples and src null cells? This antibody is generated by immunizing BALB/c mice with full length native protein (purified) and then fusing with P3X63 Ag8.653 myeloma cells. The epitope is within the SH3 domain of either v-Src or c-Src. We have no evidence for ab16885 cross-reacting with other src family kinases (e.g.fyn). I will contact the originator of ab16885 for information on platelet testing.
Asked on Sep 05 2006
Thank you for your patience. Regarding ab16885 (Mouse monoclonal [Clone 327] to Src), this antibody has not been tested on mouse platelets or src-null cells. Also, we have not tested this product for crossreactivity with other src family members e.g. lyn and fyn. However this antibody has been extensively characterized in "Lipsich, L.A. et al. 1983, J. Virol. 48, 352". This article indicates that the antibody is not cross reactive with pp90yes, pp110fps, or pp68ros. This antibody has also been cited in "Kitagawa et al. 2002, JBC 277, 366-371" and Maekawa et al. 2002, Biology of Reproduction 66, 211-221" which provides circumstantial evidence that this antibody does not crossreact with other kinases. I hope this information helps. Please do not hesitate to contact us again if you need further assistance.
Answered on Sep 06 2006