Western blot abreview for Anti-Src antibody [EPR5496]

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (colon carcinoma cells)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
50 µg
Specification
colon carcinoma cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% milk TBS Tween
Dilution
1/2000

Secondary antibody

Dilution
1/6000
Name
Non-Abcam antibody was used: Rabbit polyclonal HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ecl
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 50 kDa

Additional data

Abcam response
We appreciate the time and effort that the reviewer has put in to testing this product in this application.
PubMed ID
29954829: View

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Submitted Sep 06 2018

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