Product nameAnti-Src (phospho Y215) antibody
See all Src primary antibodies
DescriptionRabbit polyclonal to Src (phospho Y215)
Specificityab24789 recognises c-Src and it may recognise other Src family members such as hck, fyn, csk and yes when phosphorylated on their conserved SH-2 domain tyrosine site.
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Synthetic peptide (Human) corresponding to amino acids around tyrosine 215 of human Src ,conjugated to KLH
- A431 cells treated with pervanadate
This antibody is useful in the study of tyrosine kinase regulation to detect phosphorylated Src. Phosphorylation of Tyr-215 (and Tyr-418) increase tyrosine kinase activity.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesCross adsorbed to phospho-tyrosine coupled to agarose then affinity purified using phospho-c-Tyr-215 peptide, without carrier.
Primary antibody notesThis antibody is useful in the study of tyrosine kinase regulation to detect phosphorylated Src. Phosphorylation of Tyr-215 (and Tyr-418) increase tyrosine kinase activity.
Our Abpromise guarantee covers the use of ab24789 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 60 kDa.
Incubate the blotting membrane with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween 20 for 1 hour at room temperature.
FunctionNon-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function. Once it is recruited to the activated integrins, by PTK2B, it phosphorylates CBL which in turn induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. Promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase. Phosphorylates RUNX3 and COX2 on tyrosine residues, TNK2 on 'Tyr-284' and CBL on 'Tyr-731'. Enhances DDX58/RIG-I-elicited antiviral signaling.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain.
modificationsDephosphorylated at Tyr-530 by PTPRJ (By similarity). Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. Dephosphorylated by PTPRJ at Tyr-419. Normally maintained in an inactive conformation with the SH2 domain engaged with Tyr-530, the SH3 domain engaged with the SH2-kinase linker, and Tyr-419 dephosphorylated. Dephosphorylation of Tyr-530 as a result of protein tyrosine phosphatase (PTP) action disrupts the intramolecular interaction between the SH2 domain and Tyr-530, Tyr-419 can then become autophosphorylated, resulting in SRC activation. Phosphorylation of Tyr-530 by CSK allows this interaction to reform, resulting in SRC inactivation.
S-nitrosylation is important for activation of its kinase activity.
Cellular localizationCell membrane. Mitochondrion inner membrane.
- Information by UniProt
FormThis protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), FYN (P06241), YES1 (P07947), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
- ASV antibody
- Avian sarcoma virus antibody
- c SRC antibody
All lanes : Anti-Src (phospho Y215) antibody (ab24789) at 1/1000 dilution
Lane 1 : A431 cells treated with pervanadate (1 mM) for 30 minutes (20 ug/lane) with absence of blocking peptide
Lane 2 : A431 cells treated with pervanadate (1 mM) for 30 minutes (20 ug/lane) with presence of phospho-c-Src (Tyr-215) peptide
Lane 3 : A431 cells treated with pervanadate (1 mM) for 30 minutes (20 ug/lane) with presence of unrelated phospho-beta-Catenin (Tyr-142) peptide
Lane 4 : A431 cells treated with pervanadate (1 mM) for 30 minutes (20 ug/lane) with BSA conjugated to phospho-tyrosine
Predicted band size: 60 kDa
Peptides and BSA were used at 1 ug/ml. Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
This product has been referenced in:
- Zhou J et al. Black rice-derived anthocyanins inhibit HER-2-positive breast cancer epithelial-mesenchymal transition-mediated metastasis in vitro by suppressing FAK signaling. Int J Mol Med 40:1649-1656 (2017). Read more (PubMed: 29039492) »
- Smida M et al. A novel negative regulatory function of the phosphoprotein associated with glycosphingolipid-enriched microdomains: blocking Ras activation. Blood 110:596-615 (2007). Read more (PubMed: 17389760) »