• Product name
    Anti-Src (phospho Y418) antibody
    See all Src primary antibodies
  • Description
    Rabbit polyclonal to Src (phospho Y418)
  • Host species
  • Specificity
    This antibody detects endogenous levels of Src only when phosphorylated at tyrosine 418.
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from human Src around the phosphorylation site of tyrosine 418 (N-E-YP-T-A).

  • Positive control
    • Human colon carcinoma tissue; COLO205 cell extracts



Our Abpromise guarantee covers the use of ab47411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
ELISA 1/20000.
IHC-P Use at an assay dependent concentration.


  • Function
    Non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function. Once it is recruited to the activated integrins, by PTK2B, it phosphorylates CBL which in turn induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. Promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase. Phosphorylates RUNX3 and COX2 on tyrosine residues, TNK2 on 'Tyr-284' and CBL on 'Tyr-731'. Enhances DDX58/RIG-I-elicited antiviral signaling.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • Post-translational
    Dephosphorylated at Tyr-530 by PTPRJ (By similarity). Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. Dephosphorylated by PTPRJ at Tyr-419. Normally maintained in an inactive conformation with the SH2 domain engaged with Tyr-530, the SH3 domain engaged with the SH2-kinase linker, and Tyr-419 dephosphorylated. Dephosphorylation of Tyr-530 as a result of protein tyrosine phosphatase (PTP) action disrupts the intramolecular interaction between the SH2 domain and Tyr-530, Tyr-419 can then become autophosphorylated, resulting in SRC activation. Phosphorylation of Tyr-530 by CSK allows this interaction to reform, resulting in SRC inactivation.
    S-nitrosylation is important for activation of its kinase activity.
  • Cellular localization
    Cell membrane. Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Form
    This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), FYN (P06241), YES1 (P07947), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
  • Alternative names
    • ASV antibody
    • Avian sarcoma virus antibody
    • c SRC antibody
    • CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA antibody
    • cSrc antibody
    • EC antibody
    • Neuronal CSRC tyrosine specific protein kinase antibody
    • Neuronal SRC antibody
    • Oncogene SRC antibody
    • OTTHUMP00000174476 antibody
    • OTTHUMP00000174477 antibody
    • p60 Src antibody
    • p60-Src antibody
    • p60Src antibody
    • pp60c src antibody
    • pp60c-src antibody
    • pp60csrc antibody
    • Proto oncogene tyrosine protein kinase Src antibody
    • Proto-oncogene c-Src antibody
    • Proto-oncogene tyrosine-protein kinase Src antibody
    • Protooncogene SRC antibody
    • Protooncogene SRC Rous sarcoma antibody
    • Src antibody
    • SRC Oncogene antibody
    • SRC proto oncogene non receptor tyrosine kinase antibody
    • SRC_HUMAN antibody
    • SRC1 antibody
    • Tyrosine kinase pp60c src antibody
    • Tyrosine protein kinase SRC 1 antibody
    • Tyrosine protein kinase SRC1 antibody
    • v src avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog antibody
    • V src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog (avian) antibody
    • v src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog avian antibody
    see all


  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue in the presence (right) and absence (left) of blocking phosphopeptide, using ab47411 at 1/50 dilution.
  • All lanes : Anti-Src (phospho Y418) antibody (ab47411) at 1/500 dilution

    Lane 1 : COLO205 cell extracts with serum
    Lane 2 : COLO205 cell extracts

    Predicted band size: 60 kDa

    Western blot analysis of extracts from COLO205 cells in the presence (lane 2) and absence (lane 1) of serum, using ab47411 at 1/500 dilution.


This product has been referenced in:
  • Chernova T  et al. Long-Fiber Carbon Nanotubes Replicate Asbestos-Induced Mesothelioma with Disruption of the Tumor Suppressor Gene Cdkn2a (Ink4a/Arf). Curr Biol 27:3302-3314.e6 (2017). Read more (PubMed: 29112861) »
  • Donahue TR  et al. Integrative survival-based molecular profiling of human pancreatic cancer. Clin Cancer Res 18:1352-63 (2012). IHC-P ; Human . Read more (PubMed: 22261810) »

See all 2 Publications for this product

Customer reviews and Q&As

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrat pH6
Mouse Tissue sections (kidney)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Aug 20 2014

Western blot
Rat Tissue lysate - whole (Kidney)
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (10% Gel)
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Feb 12 2013

ab38497 : DISCOUNT CODE: 41002

ab47411: DISCOUNT CODE: 41864

Expiration date:09. October 2012

I am very pleased to hear your customer would like to accept our offer and test ab38497 and ab47411in mouse.

Eachcode ...

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Thank you for your enquiry.

We do not stock small sample sizes for our products. Only sizes noted on the online product datasheets are available. However, it seems you are interested in using ab47411 in IHC on paraffin embedded sections of hu...

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Thank you for your patience.

I just found out that, unfortunately, the blocking peptide for ab47411 is also not available for sale. I am very sorry to not be able to help you further in this regard.

Please do not hesitate to conta...

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Thank you for contacting us.

For ab4817, ab32078 the immuinogen peptide are unfortunately not available.

I have inquired about ab47411 and will let you know when I hear back form the lab.

Thank you for your patience.In t...

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