Recombinant
RabMAb

Recombinant Anti-Src (phospho Y418) antibody [EPR6239(2)] (ab133460)

Overview

  • Product name

    Anti-Src (phospho Y418) antibody [EPR6239(2)]
    See all Src primary antibodies
  • Description

    Rabbit monoclonal [EPR6239(2)] to Src (phospho Y418)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Src (phospho Y418).
    Database link: P12931

  • Positive control

    • A431 cell lysate treated with Pervanadate
  • General notes

    A trial size is available to purchase for this antibody.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function. Once it is recruited to the activated integrins, by PTK2B, it phosphorylates CBL which in turn induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. Promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase. Phosphorylates RUNX3 and COX2 on tyrosine residues, TNK2 on 'Tyr-284' and CBL on 'Tyr-731'. Enhances DDX58/RIG-I-elicited antiviral signaling.
    • Sequence similarities

      Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
      Contains 1 protein kinase domain.
      Contains 1 SH2 domain.
      Contains 1 SH3 domain.
    • Post-translational
      modifications

      Dephosphorylated at Tyr-530 by PTPRJ (By similarity). Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. Dephosphorylated by PTPRJ at Tyr-419. Normally maintained in an inactive conformation with the SH2 domain engaged with Tyr-530, the SH3 domain engaged with the SH2-kinase linker, and Tyr-419 dephosphorylated. Dephosphorylation of Tyr-530 as a result of protein tyrosine phosphatase (PTP) action disrupts the intramolecular interaction between the SH2 domain and Tyr-530, Tyr-419 can then become autophosphorylated, resulting in SRC activation. Phosphorylation of Tyr-530 by CSK allows this interaction to reform, resulting in SRC inactivation.
      S-nitrosylation is important for activation of its kinase activity.
    • Cellular localization

      Cell membrane. Mitochondrion inner membrane.
    • Information by UniProt
    • Database links

    • Form

      This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), FYN (P06241), YES1 (P07947), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
    • Alternative names

      • ASV antibody
      • Avian sarcoma virus antibody
      • c SRC antibody
      • CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA antibody
      • cSrc antibody
      • EC 2.7.10.2 antibody
      • Neuronal CSRC tyrosine specific protein kinase antibody
      • Neuronal SRC antibody
      • Oncogene SRC antibody
      • OTTHUMP00000174476 antibody
      • OTTHUMP00000174477 antibody
      • p60 Src antibody
      • p60-Src antibody
      • p60Src antibody
      • pp60c src antibody
      • pp60c-src antibody
      • pp60csrc antibody
      • Proto oncogene tyrosine protein kinase Src antibody
      • Proto-oncogene c-Src antibody
      • Proto-oncogene tyrosine-protein kinase Src antibody
      • Protooncogene SRC antibody
      • Protooncogene SRC Rous sarcoma antibody
      • Src antibody
      • SRC Oncogene antibody
      • SRC proto oncogene non receptor tyrosine kinase antibody
      • SRC_HUMAN antibody
      • SRC1 antibody
      • Tyrosine kinase pp60c src antibody
      • Tyrosine protein kinase SRC 1 antibody
      • Tyrosine protein kinase SRC1 antibody
      • v src avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog antibody
      • V src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog (avian) antibody
      • v src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog avian antibody
      see all

    Images

    • All lanes : Anti-Src (phospho Y418) antibody [EPR6239(2)] (ab133460) at 1/1000 dilution

      Lane 1 : Untreated C6 (rat glioma) whole cell lysate
      Lane 2 : C6 (rat glioma) treated with pervanadate whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 60 kDa



      Purified format.

      Blocking buffer: 5% NFDM/TBST

      Diluting buffer: 5% NFDM/TBST

    • All lanes : Anti-Src (phospho Y418) antibody [EPR6239(2)] (ab133460) at 1/1000 dilution

      Lane 1 : HeLa (human cervix adenocarcinoma) treated with pervanadate whole cell lysate
      Lane 2 : Untreated HeLa (human cervix adenocarcinoma) treated with pervanadate whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 60 kDa



      Purified format.

      Blocking buffer: 5% NFDM/TBST

      Diluting buffer: 5% NFDM/TBST

    • All lanes : Anti-Src (phospho Y418) antibody [EPR6239(2)] (ab133460) at 1/1000 dilution

      Lane 1 : A431 (human epidermoid carcinoma) treated with pervanadate whole cell lysate
      Lane 2 : Untreated A431 (human epidermoid carcinoma) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 60 kDa



      Purified format.

      Blocking Buffer: 5% NFDM/TBST

      Diluting buffer: 5% NFDM /TBST 

       

    • All lanes : Anti-Src (phospho Y418) antibody [EPR6239(2)] (ab133460) at 1/1000 dilution (undiluted)

      Lane 1 : A431 cell lysate untreated
      Lane 2 : A431 cell lysate treated with Pervanadate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 60 kDa

    • Undiluted format.

      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Chen YC  et al. 16-Hydroxycleroda-3,13-dien-15,16-olide induces anoikis in human renal cell carcinoma cells: involvement of focal adhesion disassembly and signaling. Onco Targets Ther 11:7679-7690 (2018). Read more (PubMed: 30464516) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (Rat primary cortical neuronal cultures)
    Gel Running Conditions
    Reduced Denaturing (15%)
    Loading amount
    25 µg
    Treatment
    treated with DMSO or staurosporine 1µM
    Specification
    Rat primary cortical neuronal cultures
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: RT°C

    Abcam user community

    Verified customer

    Submitted Mar 22 2019

    Answer

    Thank you for contacting us.

    We have not tested these antibodies in Drosophila, but BLAST searches showed low homology for both antibodies, 40-50%, so I would not predict reactivity.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

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