• Product name
    Anti-SRC3 antibody - ChIP Grade
    See all SRC3 primary antibodies
  • Description
    Rabbit polyclonal to SRC3 - ChIP Grade
  • Host species
  • Specificity
    Detects amplified in breast cancer 1 (AIB1).
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, ChIPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Xenopus laevis
  • Immunogen

    Synthetic peptide corresponding to Human SRC3 aa 3-15.


    (Peptide available as ab4915)

  • Positive control
    • T47D cell lysate


Our Abpromise guarantee covers the use of ab2831 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 155 kDa.Can be blocked with SRC3 peptide (ab4915). Can be blocked with AIB1 peptide or reuse.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
ChIP Use 3µg for 106 cells. PubMed: 18499756


  • Function
    Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit. Interacts with PSMB9.
  • Tissue specificity
    Widely expressed. High expression in heart, skeletal muscle, pancreas and placenta. Low expression in brain, and very low in lung, liver and kidney.
  • Sequence similarities
    Belongs to the SRC/p160 nuclear receptor coactivator family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 PAS (PER-ARNT-SIM) domain.
  • Domain
    Contains three Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. Motifs 1 and 2 are essential for the association with nuclear receptors, and constitute the RID domain (Receptor-interacting domain).
  • Post-translational
    Acetylated by CREBBP. Acetylation occurs in the RID domain, and disrupts the interaction with nuclear receptors and regulates its function.
    Methylated by CARM1.
    Phosphorylated by IKK complex. Regulated its function.
  • Cellular localization
    Cytoplasm. Nucleus. Mainly cytoplasmic and weakly nuclear. Upon TNF activation and subsequent phosphorylation, it translocates from the cytoplasm to the nucleus.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • ACTR antibody
    • AIB 1 antibody
    • AIB-1 antibody
    • AIB1 antibody
    • Amplified in breast cancer 1 antibody
    • Amplified in breast cancer 1 protein antibody
    • bHLHe42 antibody
    • CAGH 16 antibody
    • CAGH16 antibody
    • CBP interacting protein antibody
    • CBP-interacting protein antibody
    • Class E basic helix-loop-helix protein 42 antibody
    • CTG 26 antibody
    • CTG26 antibody
    • KAT13B / AIB1 antibody
    • MGC141848 antibody
    • NCOA 3 antibody
    • NCoA-3 antibody
    • Ncoa3 antibody
    • NCOA3_HUMAN antibody
    • Nuclear receptor coactivator 3 antibody
    • pCIP antibody
    • RAC 3 antibody
    • RAC-3 antibody
    • RAC3 antibody
    • Receptor associated coactivator 3 antibody
    • Receptor-associated coactivator 3 antibody
    • SRC 3 antibody
    • SRC-3 antibody
    • SRC3 antibody
    • Steroid receptor coactivator protein 3 antibody
    • Thyroid hormone receptor activator molecule 1 antibody
    • TNRC14 antibody
    • TNRC16 antibody
    • TRAM 1 antibody
    • TRAM-1 antibody
    • TRAM1 antibody
    see all


  • ab2831 can be used in ChIP. You can expect recruitment of AIB1on the pS2 promoter at some point during transcriptional activation. The amount of DNA precipiated is significant.

    Sonicated Chromatin prepared from untreated or 17beta-estradiol (E2) treated MCF7 cells was subjected to the ChIP procedure with ab2831 to AIB1 and the immunoprecipitated chromatin was analysed in the proximal region of the estrogen-responsive pS2 promoter (as shown above) and quantified by real-time PCR (values are % of inputs). The primers are designed to follow the nucleosome E (including the Estrogen Responsive Element ERE). 3 µl of ab2831 and 2x106 cells were used in each ChIP experiment. 

  • Western blot of Human 2831 in T47D cell lysate using ab2831.
  • ICC/IF image of ab2831 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab2831, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

  • IHC image of ab2831 staining in human melanoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2831, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


This product has been referenced in:
See all 5 Publications for this product

Customer reviews and Q&As


BATCH NUMBER 169016 ORDER NUMBER 134911 DESCRIPTION OF THE PROBLEM No signal or weak signal Only after 1 hour exposition I see a weak band with 80 ?g of a T47D lysate and a little bit stronger band with a 293T extract (transfected cells with human NCoA3). There are some strong unspecific bands at 100 kDa and higher and also at 75 kDa and below SAMPLE I used 293T and T47 cell extracts PRIMARY ANTIBODY Rabbit polycolnal to AIB1 (ab2831) in blocking solution, incuabtion overnight at 4?C dilution 1:500 and 1:000 washing: 3x with TBST DETECTION METHOD ECLplus (Amersham): incubation 5 min POSITIVE AND NEGATIVE CONTROLS USED positive control should be a T47D cell lysate (as described in your data sheet ANTIBODY STORAGE CONDITIONS Aliquots stored at -20?C SAMPLE PREPARATION Buffer: 20 mM Tris/HCl, pH 8; 100 mM NaCl, 1 mM EDTA, 1 mM DTT, 0,2% (v/v) NP-40, 10% Glycerol Protease Inhibitor: Complete EDTA-free (Roche) Phosphataseinhibitors: Phosphatase Inhibitor Cocktail 1 and 2 (Sigma) Denaturation: 5 min 95?C AMOUNT OF PROTEIN LOADED I used 80 ?g of 293T cell lysate and 20, 40 and 80 ?g of T47D cell lysate ELECTROPHORESIS/GEL CONDITIONS reducing conditions, 8% SDS gel. run with 80-120 V TRANSFER AND BLOCKING CONDITIONS transfer with a 3 buffer system, conditons: 1 h, 100 mA (PVDF membrane 9x7 cm) blocking agent: 5% milk powder in TBST (1h, room temparature) SECONDARY ANTIBODY anti rabbit HRP-linked (another company) from donkey, dilution: 1:10000 in blocking solution, incubation: 1h, room temperature washing: 3x with TBST HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 1 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? I change the dilution from 1:1000 to 1:500 ADDITIONAL NOTES I used the same antibody also in a ChIP experiment and I get also no good results, but in this case it could also be a optimization problem of my ChIP procedure

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Thank you for the protocol details that you have provided and I'm sorry to hear that you are experiencing difficulty with ab2831. The antibody was characterized for application in Western blotting using T47D cell lysate and a band at 155 kDa was detected. You certainly are loading a sufficient amount of lysate and a suggestion would be to try using nuclear extract instead of whole cell lysate, but the antibody's originator was able to detect AIB1 in the T47D cell lysate. Just to check - is your secondary antibody working properly with other primaries? There have not been any problems with the batch that you received and it sounds to me as if there may be a problem with the particular that you received. I would be happy to send you a free of charge replacement vial or can issue you a credit note or refund. Please let me know how you would like to proceed and I look forward to hearing from you.

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