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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Acetylation
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RecombinantRabMAb

Recombinant Anti-SRC3 antibody [EPR4374(3)] (ab133611)

  • Datasheet
Reviews (1) Submit a question References (1)

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Western blot - Anti-SRC3 antibody [EPR4374(3)] (ab133611)
  • Flow Cytometry - Anti-SRC3 antibody [EPR4374(3)] (ab133611)
  • Immunocytochemistry - Anti-SRC3 antibody [EPR4374(3)] (ab133611)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRC3 antibody [EPR4374(3)] (ab133611)
  • Anti-SRC3 antibody [EPR4374(3)] (ab133611)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4374(3)] to SRC3
  • Suitable for: WB, IHC-P, Flow Cyt, ICC
  • Reacts with: Human

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

View more associated products

Overview

  • Product name

    Anti-SRC3 antibody [EPR4374(3)]
    See all SRC3 primary antibodies
  • Description

    Rabbit monoclonal [EPR4374(3)] to SRC3
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to residues on the C terminus of Human KAT13B/ AIB1 (UniProt ID: Q9Y6Q9).

  • Positive control

    • Daudi, HeLa and K562 cell lysates; Human endometrium adenocarcinoma tissue
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR4374(3)
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • Nuclear Hormone Receptors
    • Co-activators/co-repressors
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Co-activators/co-repressors
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HAT
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • Nuclear hormone receptors
    • Coactivators and repressors
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Types of disease
    • Obesity

Associated products

  • Alternative Versions

    • Anti-SRC3 antibody [EPR4374(3)] - BSA and Azide free (ab243600)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa nuclear extract lysate (ab14655)
    • K-562 nuclear extract lysate (ab14851)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133611 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC
Human
IHC-P
Human
WB
Human
Application Abreviews Notes
WB (1)
1/1000 - 1/10000. Detects a band of approximately 160 kDa (predicted molecular weight: 155 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC
1/50 - 1/100.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 160 kDa (predicted molecular weight: 155 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC
1/50 - 1/100.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit. Interacts with PSMB9.
    • Tissue specificity

      Widely expressed. High expression in heart, skeletal muscle, pancreas and placenta. Low expression in brain, and very low in lung, liver and kidney.
    • Sequence similarities

      Belongs to the SRC/p160 nuclear receptor coactivator family.
      Contains 1 basic helix-loop-helix (bHLH) domain.
      Contains 1 PAS (PER-ARNT-SIM) domain.
    • Domain

      Contains three Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. Motifs 1 and 2 are essential for the association with nuclear receptors, and constitute the RID domain (Receptor-interacting domain).
    • Post-translational
      modifications

      Acetylated by CREBBP. Acetylation occurs in the RID domain, and disrupts the interaction with nuclear receptors and regulates its function.
      Methylated by CARM1.
      Phosphorylated by IKK complex. Regulated its function.
    • Cellular localization

      Cytoplasm. Nucleus. Mainly cytoplasmic and weakly nuclear. Upon TNF activation and subsequent phosphorylation, it translocates from the cytoplasm to the nucleus.
    • Target information above from: UniProt accession Q9Y6Q9 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 8202 Human
      • Omim: 601937 Human
      • SwissProt: Q9Y6Q9 Human
      • Unigene: 592142 Human
      • Alternative names

        • ACTR antibody
        • AIB 1 antibody
        • AIB-1 antibody
        • AIB1 antibody
        • Amplified in breast cancer 1 antibody
        • Amplified in breast cancer 1 protein antibody
        • bHLHe42 antibody
        • CAGH 16 antibody
        • CAGH16 antibody
        • CBP interacting protein antibody
        • CBP-interacting protein antibody
        • Class E basic helix-loop-helix protein 42 antibody
        • CTG 26 antibody
        • CTG26 antibody
        • KAT13B / AIB1 antibody
        • MGC141848 antibody
        • NCOA 3 antibody
        • NCoA-3 antibody
        • Ncoa3 antibody
        • NCOA3_HUMAN antibody
        • Nuclear receptor coactivator 3 antibody
        • pCIP antibody
        • RAC 3 antibody
        • RAC-3 antibody
        • RAC3 antibody
        • Receptor associated coactivator 3 antibody
        • Receptor-associated coactivator 3 antibody
        • SRC 3 antibody
        • SRC-3 antibody
        • SRC3 antibody
        • Steroid receptor coactivator protein 3 antibody
        • Thyroid hormone receptor activator molecule 1 antibody
        • TNRC14 antibody
        • TNRC16 antibody
        • TRAM 1 antibody
        • TRAM-1 antibody
        • TRAM1 antibody
        see all

      Images

      • Western blot - Anti-SRC3 antibody [EPR4374(3)] (ab133611)
        Western blot - Anti-SRC3 antibody [EPR4374(3)] (ab133611)
        All lanes : Anti-SRC3 antibody [EPR4374(3)] (ab133611) at 1/1000 dilution

        Lane 1 : Daudi cell lysate
        Lane 2 : HeLa cell lysate
        Lane 3 : K562 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 155 kDa

      • Flow Cytometry - Anti-SRC3 antibody [EPR4374(3)] (ab133611)
        Flow Cytometry - Anti-SRC3 antibody [EPR4374(3)] (ab133611)
        Overlay histogram showing HeLa cells stained with ab133611 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133611, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
      • Immunocytochemistry - Anti-SRC3 antibody [EPR4374(3)] (ab133611)
        Immunocytochemistry - Anti-SRC3 antibody [EPR4374(3)] (ab133611)
        Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) labeling SRC3 with purified ab133611 at 1/100 dilution. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.29 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
        Negative control: PBS instead of the primary antibody.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRC3 antibody [EPR4374(3)] (ab133611)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRC3 antibody [EPR4374(3)] (ab133611)

        Immunohistochemical analysis of KAT13B / AIB1 in paraffin embedded Human endometrium adenocarcinoma tissue, using ab133611 at a dilution of 1/50.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Anti-SRC3 antibody [EPR4374(3)] (ab133611)
        Anti-SRC3 antibody [EPR4374(3)] (ab133611)

      Protocols

      • Flow cytometry protocols
      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols
      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (1)

      Publishing research using ab133611? Please let us know so that we can cite the reference in this datasheet.

      ab133611 has been referenced in 1 publication.

      • Gee F  et al. Correlation of the osteoarthritis susceptibility variants that map to chromosome 20q13 with an expression quantitative trait locus operating on NCOA3 and with functional variation at the polymorphism rs116855380. Arthritis Rheumatol 67:2923-32 (2015). PubMed: 26211391

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Western blot abreview for Anti-SRC3 antibody [EPR4374(3)]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (HeLa)
      Loading amount
      50 µg
      Specification
      HeLa
      Gel Running Conditions
      Reduced Denaturing (4-15% gel)
      Blocking step
      Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      Submitted Jul 20 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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