RecombinantRabMAb

Recombinant Anti-SRC3 antibody [EPR4374(3)] - BSA and Azide free (ab243600)

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Western blot - Anti-SRC3 antibody [EPR4374(3)] - BSA and Azide free (ab243600)
  • Flow Cytometry - Anti-SRC3 antibody [EPR4374(3)] - BSA and Azide free (ab243600)
  • Immunocytochemistry - Anti-SRC3 antibody [EPR4374(3)] - BSA and Azide free (ab243600)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRC3 antibody [EPR4374(3)] - BSA and Azide free (ab243600)
  • Anti-SRC3 antibody [EPR4374(3)] - BSA and Azide free (ab243600)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4374(3)] to SRC3 - BSA and Azide free
  • Suitable for: Flow Cyt, IHC-P, WB, ICC
  • Reacts with: Human

Overview

  • Product name

    Anti-SRC3 antibody [EPR4374(3)] - BSA and Azide free
    See all SRC3 primary antibodies

  • Description

    Rabbit monoclonal [EPR4374(3)] to SRC3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WB, ICCmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human SRC3 aa 1400 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q9Y6Q9

  • Positive control

    • Daudi, HeLa and K562 cell lysates; Human endometrium adenocarcinoma tissue

  • General notes

    ab243600 is the carrier-free version of ab133611 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab243600 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Applications

Our Abpromise guarantee covers the use of ab243600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 160 kDa (predicted molecular weight: 155 kDa).
ICC Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function

      Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit. Interacts with PSMB9.

    • Tissue specificity

      Widely expressed. High expression in heart, skeletal muscle, pancreas and placenta. Low expression in brain, and very low in lung, liver and kidney.

    • Sequence similarities

      Belongs to the SRC/p160 nuclear receptor coactivator family.
      Contains 1 basic helix-loop-helix (bHLH) domain.
      Contains 1 PAS (PER-ARNT-SIM) domain.

    • Domain

      Contains three Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. Motifs 1 and 2 are essential for the association with nuclear receptors, and constitute the RID domain (Receptor-interacting domain).

    • Post-translational
      modifications

      Acetylated by CREBBP. Acetylation occurs in the RID domain, and disrupts the interaction with nuclear receptors and regulates its function.
      Methylated by CARM1.
      Phosphorylated by IKK complex. Regulated its function.

    • Cellular localization

      Cytoplasm. Nucleus. Mainly cytoplasmic and weakly nuclear. Upon TNF activation and subsequent phosphorylation, it translocates from the cytoplasm to the nucleus.
    • Information by UniProt

    • Database links

      • Alternative names

        • ACTR antibody
        • AIB 1 antibody
        • AIB-1 antibody
        • AIB1 antibody
        • Amplified in breast cancer 1 antibody
        • Amplified in breast cancer 1 protein antibody
        • bHLHe42 antibody
        • CAGH 16 antibody
        • CAGH16 antibody
        • CBP interacting protein antibody
        • CBP-interacting protein antibody
        • Class E basic helix-loop-helix protein 42 antibody
        • CTG 26 antibody
        • CTG26 antibody
        • KAT13B / AIB1 antibody
        • MGC141848 antibody
        • NCOA 3 antibody
        • NCoA-3 antibody
        • Ncoa3 antibody
        • NCOA3_HUMAN antibody
        • Nuclear receptor coactivator 3 antibody
        • pCIP antibody
        • RAC 3 antibody
        • RAC-3 antibody
        • RAC3 antibody
        • Receptor associated coactivator 3 antibody
        • Receptor-associated coactivator 3 antibody
        • SRC 3 antibody
        • SRC-3 antibody
        • SRC3 antibody
        • Steroid receptor coactivator protein 3 antibody
        • Thyroid hormone receptor activator molecule 1 antibody
        • TNRC14 antibody
        • TNRC16 antibody
        • TRAM 1 antibody
        • TRAM-1 antibody
        • TRAM1 antibody
        see all

      Images

      • Western blot - Anti-SRC3 antibody [EPR4374(3)] - BSA and Azide free (ab243600)
        Western blot - Anti-SRC3 antibody [EPR4374(3)] - BSA and Azide free (ab243600)
        All lanes : Anti-SRC3 antibody [EPR4374(3)] (ab133611) at 1/1000 dilution

        Lane 1 : Daudi cell lysate
        Lane 2 : HeLa cell lysate
        Lane 3 : K562 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 155 kDa



        This data was developed using ab133611, the same antibody clone in a different buffer formulation.

      • Flow Cytometry - Anti-SRC3 antibody [EPR4374(3)] - BSA and Azide free (ab243600)
        Flow Cytometry - Anti-SRC3 antibody [EPR4374(3)] - BSA and Azide free (ab243600)
        This data was developed using ab133611, the same antibody clone in a different buffer formulation.Overlay histogram showing HeLa cells stained with ab133611 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133611, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1?g/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
      • Immunocytochemistry - Anti-SRC3 antibody [EPR4374(3)] - BSA and Azide free (ab243600)
        Immunocytochemistry - Anti-SRC3 antibody [EPR4374(3)] - BSA and Azide free (ab243600)
        This data was developed using the same antibody clone in a different buffer formulation (ab133611 ).
        Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) labeling SRC3 with purified ab133611 at 1/100 dilution. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.29 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
        Negative control: PBS instead of the primary antibody.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRC3 antibody [EPR4374(3)] - BSA and Azide free (ab243600)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRC3 antibody [EPR4374(3)] - BSA and Azide free (ab243600)
        This data was developed using ab133611, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of KAT13B / AIB1 in paraffin embedded Human endometrium adenocarcinoma tissue, using ab133611 at a dilution of 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
      • Anti-SRC3 antibody [EPR4374(3)] - BSA and Azide free (ab243600)
        Anti-SRC3 antibody [EPR4374(3)] - BSA and Azide free (ab243600)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab243600? Please let us know so that we can cite the reference in this datasheet.

      ab243600 has not yet been referenced specifically in any publications.

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