Overview

  • Product name
  • Description
    Goat polyclonal to SRCAP
  • Host species
    Goat
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog
  • Immunogen

    Synthetic peptide corresponding to Human SRCAP aa 746-758 (internal sequence).
    Sequence:

    C-DEAQNIKNFKSQR


    Database link: NP_006653.2

  • Positive control
    • ICC/IF: A431 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab99408 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB Use at an assay dependent concentration. Predicted molecular weight: 344 kDa. PubMed: 24163101

Preliminary experiments in lysates of cell lines K562 and MOLT4 gave no specific signal but low background (at antibody concentration up to 1µg/ml).

Target

  • Relevance
    Catalytic component of the SRCAP complex which mediates the ATP-dependent exchange of histone H2AZ/H2B dimers for nucleosomal H2A/H2B, leading to transcriptional regulation of selected genes by chromatin remodeling. Acts as a coactivator for CREB-mediated transcription, steroid receptor-mediated transcription, and Notch-mediated transcription (from SwissProt).
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • domino homolog 2 antibody
    • DOMO1 antibody
    • EAF1 antibody
    • FLHS antibody
    • Helicase SRCAP antibody
    • KIAA0309 antibody
    • Snf2 related CBP activator protein antibody
    • Swi2/Snf2-related ATPase homolog, domino homolog 1 antibody
    • SWR1 antibody
    • SWR1, S. cerevisiae, homologof antibody
    see all

Images

  • Immunocytochemistry/Immunofluorescence analysis of A431 cells labelling SRCAP with ab99408 at 10 µg/ml. Cells were paraformaldehyde fixed and permeabilized with 0.15% triton. Primary antibody icubation was for 1 hour followed by incubation with an Alexa Fluor® 488-conjugated secondary antibody at 2 µg/ml. DAPI nuclear counterstain was used.

    Negative control: Unimmunized goat IgG (10 µg/ml) followed by an Alexa Fluor® 488-conjugated secondary antibody (2 µg/ml).

References

This product has been referenced in:
  • Seo J  et al. Genome-wide reorganization of histone H2AX toward particular fragile sites on cell activation. Nucleic Acids Res N/A:N/A (2013). WB ; Human . Read more (PubMed: 24163101) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for contacting us with your question.

Ab99408 is produced outside of the US, so unfortunately we are not able to ship this product into Canada.

I am sorry for the inconvenience, but if there is anything else that we can do for you please let me know and I'll be happy to help.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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