Key features and details
- Goat polyclonal to SRCAP
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SRCAP antibody
See all SRCAP primary antibodies
DescriptionGoat polyclonal to SRCAP
Tested applicationsSuitable for: ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Dog
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab99408 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab99408 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 10 µg/ml.
Use a concentration of 10 µg/ml.
RelevanceCatalytic component of the SRCAP complex which mediates the ATP-dependent exchange of histone H2AZ/H2B dimers for nucleosomal H2A/H2B, leading to transcriptional regulation of selected genes by chromatin remodeling. Acts as a coactivator for CREB-mediated transcription, steroid receptor-mediated transcription, and Notch-mediated transcription (from SwissProt).
- domino homolog 2 antibody
- DOMO1 antibody
- EAF1 antibody
Immunocytochemistry/Immunofluorescence analysis of A431 cells labelling SRCAP with ab99408 at 10 µg/ml. Cells were paraformaldehyde fixed and permeabilized with 0.15% triton. Primary antibody icubation was for 1 hour followed by incubation with an Alexa Fluor® 488-conjugated secondary antibody at 2 µg/ml. DAPI nuclear counterstain was used.
Negative control: Unimmunized goat IgG (10 µg/ml) followed by an Alexa Fluor® 488-conjugated secondary antibody (2 µg/ml).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab99408 has been referenced in 3 publications.
- Greenberg RS et al. Single Amino Acid Change Underlies Distinct Roles of H2A.Z Subtypes in Human Syndrome. Cell 178:1421-1436.e24 (2019). PubMed: 31491386
- Murphy PJ et al. Placeholder Nucleosomes Underlie Germline-to-Embryo DNA Methylation Reprogramming. Cell 172:993-1006.e13 (2018). PubMed: 29456083
- Seo J et al. Genome-wide reorganization of histone H2AX toward particular fragile sites on cell activation. Nucleic Acids Res N/A:N/A (2013). WB ; Human . PubMed: 24163101