Anti-SRCAP antibody (ab99408)
Key features and details
- Goat polyclonal to SRCAP
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SRCAP antibody
See all SRCAP primary antibodies -
Description
Goat polyclonal to SRCAP -
Host species
Goat -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Dog -
Immunogen
Synthetic peptide corresponding to Human SRCAP aa 700-800 (internal sequence).
Database link: NP_006653.2 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab99408 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab99408 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Relevance
Catalytic component of the SRCAP complex which mediates the ATP-dependent exchange of histone H2AZ/H2B dimers for nucleosomal H2A/H2B, leading to transcriptional regulation of selected genes by chromatin remodeling. Acts as a coactivator for CREB-mediated transcription, steroid receptor-mediated transcription, and Notch-mediated transcription (from SwissProt). -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 10847 Human
- Entrez Gene: 100043597 Mouse
- Entrez Gene: 361652 Rat
- Omim: 611421 Human
- SwissProt: Q6ZRS2 Human
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Alternative names
- domino homolog 2 antibody
- DOMO1 antibody
- EAF1 antibody
see all
Images
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Immunocytochemistry/Immunofluorescence analysis of A431 cells labelling SRCAP with ab99408 at 10 µg/ml. Cells were paraformaldehyde fixed and permeabilized with 0.15% triton. Primary antibody icubation was for 1 hour followed by incubation with an Alexa Fluor® 488-conjugated secondary antibody at 2 µg/ml. DAPI nuclear counterstain was used.
Negative control: Unimmunized goat IgG (10 µg/ml) followed by an Alexa Fluor® 488-conjugated secondary antibody (2 µg/ml).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (5)
ab99408 has been referenced in 5 publications.
- Liu X et al. LINC00839 promotes colorectal cancer progression by recruiting RUVBL1/Tip60 complexes to activate NRF1. EMBO Rep 23:e54128 (2022). PubMed: 35876654
- Moreno-Andrés D et al. VPS72/YL1-Mediated H2A.Z Deposition Is Required for Nuclear Reassembly after Mitosis. Cells 9:N/A (2020). PubMed: 32708675
- Greenberg RS et al. Single Amino Acid Change Underlies Distinct Roles of H2A.Z Subtypes in Human Syndrome. Cell 178:1421-1436.e24 (2019). PubMed: 31491386
- Murphy PJ et al. Placeholder Nucleosomes Underlie Germline-to-Embryo DNA Methylation Reprogramming. Cell 172:993-1006.e13 (2018). PubMed: 29456083
- Seo J et al. Genome-wide reorganization of histone H2AX toward particular fragile sites on cell activation. Nucleic Acids Res N/A:N/A (2013). WB ; Human . PubMed: 24163101