Product nameAnti-SRCAP antibody
See all SRCAP primary antibodies
DescriptionGoat polyclonal to SRCAP
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Dog
- ICC/IF: A431 cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab99408 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab99408 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 10 µg/ml.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 344 kDa. PubMed: 24163101
Preliminary experiments in lysates of cell lines K562 and MOLT4 gave no specific signal but low background (at antibody concentration up to 1µg/ml).
RelevanceCatalytic component of the SRCAP complex which mediates the ATP-dependent exchange of histone H2AZ/H2B dimers for nucleosomal H2A/H2B, leading to transcriptional regulation of selected genes by chromatin remodeling. Acts as a coactivator for CREB-mediated transcription, steroid receptor-mediated transcription, and Notch-mediated transcription (from SwissProt).
- domino homolog 2 antibody
- DOMO1 antibody
- EAF1 antibody
Immunocytochemistry/Immunofluorescence analysis of A431 cells labelling SRCAP with ab99408 at 10 µg/ml. Cells were paraformaldehyde fixed and permeabilized with 0.15% triton. Primary antibody icubation was for 1 hour followed by incubation with an Alexa Fluor® 488-conjugated secondary antibody at 2 µg/ml. DAPI nuclear counterstain was used.
Negative control: Unimmunized goat IgG (10 µg/ml) followed by an Alexa Fluor® 488-conjugated secondary antibody (2 µg/ml).
This product has been referenced in:
- Murphy PJ et al. Placeholder Nucleosomes Underlie Germline-to-Embryo DNA Methylation Reprogramming. Cell 172:993-1006.e13 (2018). Read more (PubMed: 29456083) »
- Seo J et al. Genome-wide reorganization of histone H2AX toward particular fragile sites on cell activation. Nucleic Acids Res N/A:N/A (2013). WB ; Human . Read more (PubMed: 24163101) »