Overview

  • Product name

  • Description

    Goat polyclonal to SRD5A1
  • Host species

    Goat
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to Human SRD5A1 aa 173-184 (internal sequence).
    Sequence:

    RNLRKPGDTGYK


    Database link: P18405

  • Positive control

    • Human small intestine, liver and prostate tissues

Properties

Applications

Our Abpromise guarantee covers the use of ab110123 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P Use a concentration of 3.75 µg/ml. ab110123 was validated for use in Immunohistochemistry on a panel of 21 formalin fixed, paraffin embedded Human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with a biotinylated Goat IgG secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity.
WB Use at an assay dependent concentration. Predicted molecular weight: 29 kDa. PubMed: 23405234

Target

  • Function

    Converts testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.
  • Tissue specificity

    Liver and prostate (at a low level).
  • Sequence similarities

    Belongs to the steroid 5-alpha reductase family.
  • Cellular localization

    Microsome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 3 oxo 5 alpha steroid 4 dehydrogenase 1 antibody
    • 3 oxo 5 alpha steroid delta 4 dehydrogenase alpha 1 antibody
    • 3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1,5-alpha reductase antibody
    • 3-oxo-5-alpha-steroid 4-dehydrogenase 1 antibody
    • 5 alpha reductase antibody
    • S5A1_HUMAN antibody
    • S5AR 1 antibody
    • S5AR antibody
    • SR type 1 antibody
    • SRD5A 1 antibody
    • Srd5a1 antibody
    • Steroid 5 alpha reductase 1 antibody
    • Steroid 5 alpha reductase alpha polypeptide 1 (3 oxo 5 alpha steroid delta 4 dehydrogenase alpha 1) antibody
    • Steroid 5 alpha reductase alpha polypeptide 1 antibody
    • Steroid 5 alpha reductase type I antibody
    • Steroid 5-alpha-reductase 1 antibody
    see all

Images

  • Immunohistochemical analyis of SRD5A1 expression in formalin fixed, paraffin embedded Human small intestine, using ab110123 at 3.75 µg/ml.
  • Immunohistochemical analyis of SRD5A1 expression in formalin fixed, paraffin embedded Human liver, using ab110123 at 3.75 µg/ml.
  • Immunohistochemical analyis of SRD5A1 expression in formalin fixed, paraffin embedded Human prostate, using ab110123 at 3.75 µg/ml.

References

This product has been referenced in:

  • Sánchez P  et al. Effects of different ethanol-administration regimes on mRNA and protein levels of steroid 5a-reductase isozymes in prefrontal cortex of adolescent male rats. Psychopharmacology (Berl) 231:3273-80 (2014). Rat . Read more (PubMed: 24714925) »
  • Castro B  et al. Bisphenol A exposure during adulthood alters expression of aromatase and 5a-reductase isozymes in rat prostate. PLoS One 8:e55905 (2013). WB ; Rat . Read more (PubMed: 23405234) »
See all 3 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (RWPE - prostate cells)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
RWPE - prostate cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde

Dr. Armen Petrosyan

Verified customer

Submitted Mar 28 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Rat hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde

Dr. Armen Petrosyan

Verified customer

Submitted Mar 28 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Prostate LNCaP cells)
Specification
Prostate LNCaP cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.2% Triton-X
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 17 2012

Answer

Thank you for contacting us.


I have returned your reviews to editing status to allow you to make any necessary changes. You may use the codes once you have made the edits and resubmitted the Abreviews.


I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

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Question
Answer

I am very pleased to hear you would like to accept our offer and test ab110123 and ab27469 in IF. This code will give you: 1 free primary antibody per code before the expiration date. To redeem this offer, please submit an Abreview for IF and include this code in the Additional Comments section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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