• Product name

  • Description

    Goat polyclonal to SRD5A2
  • Host species

  • Specificity

    ab27469 recognises SRD5A2.
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide:


    , corresponding to N terminal amino acids 2-13 of Human SRD5A2

  • Positive control

    • Human testes or prostate lysate.
  • General notes





Our Abpromise guarantee covers the use of ab27469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 28 kDa).
ICC/IF Use at an assay dependent concentration.


  • Function

    Converts testosterone (T) into 5-alpha-dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.
  • Tissue specificity

    Expressed in high levels in the prostate and many other androgen-sensitive tissues.
  • Involvement in disease

    Defects in SRD5A2 are the cause of pseudovaginal perineoscrotal hypospadias (PPSH) [MIM:264600]. A form of male pseudohermaphroditism in which 46,XY males show ambiguous genitalia at birth, including perineal hypospadias and a blind perineal pouch, and develop masculinization at puberty. The name of the disorder stems from the finding of a blind-ending perineal opening resembling a vagina and a severely hypospadiac penis with the urethra opening onto the perineum.
  • Sequence similarities

    Belongs to the steroid 5-alpha reductase family.
  • Cellular localization

    Microsome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 3 oxo 5 alpha steroid 4 dehydrogenase 2 antibody
    • 3-oxo-5-alpha-steroid 4-dehydrogenase 2 antibody
    • 5 alpha SR2 antibody
    • 5 alpha-SR2 antibody
    • 5ART2 antibody
    • e II 5 alpha reductase antibody
    • EC antibody
    • MGC138457 antibody
    • Micropenis, included antibody
    • S5A2_HUMAN antibody
    • S5AR 2 antibody
    • SR type 2 antibody
    • SRD5A2 antibody
    • Steroid 5 alpha reductase 2 antibody
    • Steroid 5-alpha-reductase 2 antibody
    • Steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2) antibody
    • Type II 5-alpha reductase antibody
    see all


  • Anti-SRD5A2 antibody (ab27469) at 0.3 µg/ml + Human testes lysate (35ug protein in RIPA buffer)

    Predicted band size: 28 kDa
    Observed band size: 26 kDa
    why is the actual band size different from the predicted?

    Primary incubation was 1 hour. Detected by chemiluminescence.


This product has been referenced in:

  • Kelleher MA  et al. Changes in neuroactive steroid concentrations after preterm delivery in the Guinea pig. Reprod Sci 20:1365-75 (2013). Read more (PubMed: 23585339) »
  • Kelleher MA  et al. Sex-dependent effect of a low neurosteroid environment and intrauterine growth restriction on foetal guinea pig brain development. J Endocrinol 208:301-9 (2011). Read more (PubMed: 21149437) »
See all 3 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence
Human Cell (Prostate LNCaP cells)
Prostate LNCaP cells
Yes - spot 0.2% Triton-X
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 17 2012


Thank you for contacting us.

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I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

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