Overview

  • Product name
  • Description
    Goat polyclonal to SRD5A2
  • Host species
    Goat
  • Specificity
    ab27469 recognises SRD5A2.
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    QVQCQQSPVLAG

    , corresponding to N terminal amino acids 2-13 of Human SRD5A2

  • Positive control
    • Human testes or prostate lysate.
  • General notes

     

     

Properties

Applications

Our Abpromise guarantee covers the use of ab27469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 28 kDa).Can be blocked with SRD5A2 peptide (2-13) (ab45681).
ICC/IF Use at an assay dependent concentration.

Target

  • Function
    Converts testosterone (T) into 5-alpha-dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.
  • Tissue specificity
    Expressed in high levels in the prostate and many other androgen-sensitive tissues.
  • Involvement in disease
    Defects in SRD5A2 are the cause of pseudovaginal perineoscrotal hypospadias (PPSH) [MIM:264600]. A form of male pseudohermaphroditism in which 46,XY males show ambiguous genitalia at birth, including perineal hypospadias and a blind perineal pouch, and develop masculinization at puberty. The name of the disorder stems from the finding of a blind-ending perineal opening resembling a vagina and a severely hypospadiac penis with the urethra opening onto the perineum.
  • Sequence similarities
    Belongs to the steroid 5-alpha reductase family.
  • Cellular localization
    Microsome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3 oxo 5 alpha steroid 4 dehydrogenase 2 antibody
    • 3-oxo-5-alpha-steroid 4-dehydrogenase 2 antibody
    • 5 alpha SR2 antibody
    • 5 alpha-SR2 antibody
    • 5ART2 antibody
    • e II 5 alpha reductase antibody
    • EC antibody
    • MGC138457 antibody
    • Micropenis, included antibody
    • S5A2_HUMAN antibody
    • S5AR 2 antibody
    • SR type 2 antibody
    • SRD5A2 antibody
    • Steroid 5 alpha reductase 2 antibody
    • Steroid 5-alpha-reductase 2 antibody
    • Steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2) antibody
    • Type II 5-alpha reductase antibody
    see all

Images

  • Anti-SRD5A2 antibody (ab27469) at 0.3 µg/ml + Human testes lysate (35ug protein in RIPA buffer)

    Predicted band size: 28 kDa
    Observed band size: 26 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour. Detected by chemiluminescence.

References

This product has been referenced in:
  • Kelleher MA  et al. Changes in neuroactive steroid concentrations after preterm delivery in the Guinea pig. Reprod Sci 20:1365-75 (2013). Read more (PubMed: 23585339) »
  • Kelleher MA  et al. Sex-dependent effect of a low neurosteroid environment and intrauterine growth restriction on foetal guinea pig brain development. J Endocrinol 208:301-9 (2011). Read more (PubMed: 21149437) »
See all 3 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Prostate LNCaP cells)
Specification
Prostate LNCaP cells
Fixative
Paraformaldehyde
Permeabilization
Yes - spot 0.2% Triton-X
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 17 2012

Answer

Thank you for contacting us.


I have returned your reviews to editing status to allow you to make any necessary changes. You may use the codes once you have made the edits and resubmitted the Abreviews.


I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

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Question
Answer

I am very pleased to hear you would like to accept our offer and test ab110123 and ab27469 in IF. This code will give you: 1 free primary antibody per code before the expiration date. To redeem this offer, please submit an Abreview for IF and include this code in the Additional Comments section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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