Product nameAnti-SRD5A2 antibody [EPR6281(B)]
See all SRD5A2 primary antibodies
DescriptionRabbit monoclonal [EPR6281(B)] to SRD5A2
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P
Species reactivityReacts with: Rat, Human
Synthetic peptide corresponding to residues near the N-terminus of Human SRD5A2 (P31213).
- LnCaP, Human testis, and PC-3 cell lysates
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Dissociation constant (KD)KD = 3.55 x 10 -10 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124877 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).|
|IP||1/10 - 1/100.|
FunctionConverts testosterone (T) into 5-alpha-dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.
Tissue specificityExpressed in high levels in the prostate and many other androgen-sensitive tissues.
Involvement in diseaseDefects in SRD5A2 are the cause of pseudovaginal perineoscrotal hypospadias (PPSH) [MIM:264600]. A form of male pseudohermaphroditism in which 46,XY males show ambiguous genitalia at birth, including perineal hypospadias and a blind perineal pouch, and develop masculinization at puberty. The name of the disorder stems from the finding of a blind-ending perineal opening resembling a vagina and a severely hypospadiac penis with the urethra opening onto the perineum.
Sequence similaritiesBelongs to the steroid 5-alpha reductase family.
Cellular localizationMicrosome membrane. Endoplasmic reticulum membrane.
- Information by UniProt
- 3 oxo 5 alpha steroid 4 dehydrogenase 2 antibody
- 3-oxo-5-alpha-steroid 4-dehydrogenase 2 antibody
- 5 alpha SR2 antibody
All lanes : Anti-SRD5A2 antibody [EPR6281(B)] (ab124877) at 1/1000 dilution
Lane 1 : LnCaP cell lysate
Lane 2 : Human testis lysate
Lane 3 : PC3 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 28 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab124877 has not yet been referenced specifically in any publications.