Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6281(B)] to SRD5A2
- Suitable for: WB
- Reacts with: Rat, Human
Product nameAnti-SRD5A2 antibody [EPR6281(B)]
See all SRD5A2 primary antibodies
DescriptionRabbit monoclonal [EPR6281(B)] to SRD5A2
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P
Species reactivityReacts with: Rat, Human
Synthetic peptide corresponding to residues near the N-terminus of Human SRD5A2 (P31213).
- LnCaP, Human testis, and PC-3 cell lysates
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Dissociation constant (KD)KD = 3.55 x 10 -10 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124877 in the following tested applications.
|WB||1/1000 - 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).|
FunctionConverts testosterone (T) into 5-alpha-dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.
Tissue specificityExpressed in high levels in the prostate and many other androgen-sensitive tissues.
Involvement in diseaseDefects in SRD5A2 are the cause of pseudovaginal perineoscrotal hypospadias (PPSH) [MIM:264600]. A form of male pseudohermaphroditism in which 46,XY males show ambiguous genitalia at birth, including perineal hypospadias and a blind perineal pouch, and develop masculinization at puberty. The name of the disorder stems from the finding of a blind-ending perineal opening resembling a vagina and a severely hypospadiac penis with the urethra opening onto the perineum.
Sequence similaritiesBelongs to the steroid 5-alpha reductase family.
Cellular localizationMicrosome membrane. Endoplasmic reticulum membrane.
- Information by UniProt
- 3 oxo 5 alpha steroid 4 dehydrogenase 2 antibody
- 3-oxo-5-alpha-steroid 4-dehydrogenase 2 antibody
- 5 alpha SR2 antibody
All lanes : Anti-SRD5A2 antibody [EPR6281(B)] (ab124877) at 1/1000 dilution
Lane 1 : LnCaP cell lysate
Lane 2 : Human testis lysate
Lane 3 : PC3 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 28 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab124877 has been referenced in 1 publication.
- Choi YJ et al. In Vivo Effects of Polymerized Anthocyanin from Grape Skin on Benign Prostatic Hyperplasia. Nutrients 11:N/A (2019). PubMed: 31615010