Overview

  • Product name

    Anti-SREBP1 (phospho S439) antibody
    See all SREBP1 primary antibodies
  • Description

    Rabbit polyclonal to SREBP1 (phospho S439)
  • Host species

    Rabbit
  • Specificity

    ab138663 detects endogenous levels of SREBP1 only when phosphorylated at Serine 439
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic phospho peptide derived from the internal region of Human SREBP1 around the phosphorylation site of Serine 439

  • Positive control

    • Jurkat cell extracts treated with TNF at 20 ng/ml for 30 minutes

Properties

Applications

Our Abpromise guarantee covers the use of ab138663 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 122 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway (By similarity). Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3').
  • Tissue specificity

    Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1C predominates in liver, adrenal gland and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines. Isoform SREBP-1A and isoform SREBP-1C are found in kidney, brain, white fat, and muscle.
  • Sequence similarities

    Belongs to the SREBP family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Post-translational
    modifications

    At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7.
    Phosphorylated by AMPK, leading to suppress protein processing and nuclear translocation, and repress target gene expression. Phosphorylation at Ser-402 by SIK1 represses activity possibly by inhibiting DNA-binding.
  • Cellular localization

    Nucleus and Endoplasmic reticulum membrane. Golgi apparatus membrane. Cytoplasmic vesicle > COPII-coated vesicle membrane. Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.
  • Information by UniProt
  • Database links

  • Alternative names

    • ADD 1 antibody
    • bHLHd1 antibody
    • Class D basic helix-loop-helix protein 1 antibody
    • D630008H06 antibody
    • Processed sterol regulatory element-binding protein 1 antibody
    • SRBP1_HUMAN antibody
    • SREBF 1 antibody
    • SREBF1 antibody
    • SREBP 1 antibody
    • SREBP 1c antibody
    • SREBP-1 antibody
    • SREBP1 antibody
    • Sterol regulatory element binding protein 1 antibody
    • Sterol Regulatory Element Binding Transcription Factor 1 / Protein 1 antibody
    • Sterol regulatory element binding transcription factor 1 antibody
    • Sterol regulatory element-binding transcription factor 1 antibody
    see all

Images

  • Anti-SREBP1 (phospho S439) antibody (ab138663) at 1/500 dilution + Jurkat cells extracts (treated with TNF 20ng/ml for 30 mins) at 30 µg

    Predicted band size: 122 kDa

References

This product has been referenced in:

  • Lumbera WM  et al. Heat Shock Protein Augmentation of Angelica gigas Nakai Root Hot Water Extract on Adipogenic Differentiation in Murine 3T3-L1 Preadipocytes. Asian-Australas J Anim Sci 29:419-27 (2016). WB . Read more (PubMed: 26950875) »
  • Wang YG & Yang TL Liraglutide reduces fatty degeneration in hepatic cells via the AMPK/SREBP1 pathway. Exp Ther Med 10:1777-1783 (2015). WB ; Human . Read more (PubMed: 26640549) »
See all 2 Publications for this product

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