• Product name

  • Description

    Rabbit polyclonal to SREBP2
  • Host species

  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human SREBP2 aa 300-350.
    Database link: Q12772

  • Positive control

    • HeLa and 293T whole cell lysate


Our Abpromise guarantee covers the use of ab112046 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 124 kDa.
IP Use at 2-10 µg/mg of lysate.


  • Function

    Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the cholesterol and to a lesser degree the fatty acid synthesis pathway (By similarity). Binds the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') found in the flanking region of the LDRL and HMG-CoA synthase genes.
  • Tissue specificity

    Ubiquitously expressed in adult and fetal tissues.
  • Sequence similarities

    Belongs to the SREBP family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Post-translational

    At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7.
  • Cellular localization

    Nucleus and Endoplasmic reticulum membrane. Golgi apparatus membrane. Cytoplasmic vesicle > COPII-coated vesicle membrane. Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.
  • Information by UniProt
  • Database links

  • Alternative names

    • AI608257 antibody
    • bHLHd2 antibody
    • Class D basic helix-loop-helix protein 2 antibody
    • OTTHUMP00000028740 antibody
    • Processed sterol regulatory element-binding protein 2 antibody
    • SRBP2_HUMAN antibody
    • SREBF 2 antibody
    • Srebf2 antibody
    • SREBF2 protein antibody
    • SREBP 2 antibody
    • SREBP-2 antibody
    • SREBP2 antibody
    • SREBP2gc antibody
    • sterol regulatory element binding factor 2 antibody
    • Sterol regulatory element binding protein 2 antibody
    • Sterol regulatory element binding transcription factor 2 antibody
    • Sterol regulatory element-binding transcription factor 2 antibody
    see all


  • All lanes : Anti-SREBP2 antibody (ab112046) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 124 kDa

    Exposure time: 3 minutes
  • Detection of SREBP2 by Western Blot of Immunprecipitate.
    Lane 1: ab112046 at 1µg/ml staining SREBP2 in HeLa whole cell lysate immunoprecipitated using ab112046 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Lane 2: Control IgG
    Detection: Chemiluminescence with exposure time of 30 seconds.


This product has been referenced in:

  • Li X  et al. SREBP-2 promotes stem cell-like properties and metastasis by transcriptional activation of c-Myc in prostate cancer. Oncotarget 7:12869-84 (2016). WB . Read more (PubMed: 26883200) »
  • Li X  et al. Fatostatin displays high antitumor activity in prostate cancer by blocking SREBP-regulated metabolic pathways and androgen receptor signaling. Mol Cancer Ther 13:855-66 (2014). Read more (PubMed: 24493696) »
See all 2 Publications for this product

Customer reviews and Q&As

Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Denaturing (10%)
Human Cell lysate - whole cell (prostate cancer cells)
prostate cancer cells
different concentration of fatostatin for 24hrs
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Ms. Xiangyan Li

Verified customer

Submitted Jun 27 2014

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