Anti-SREBP2 antibody (ab228653)

Rabbit polyclonal SREBP2 antibody. Validated in WB, ICC/IF and tested in Human. Immunogen corresponding to recombinant fragment.

Overview

  • Product name

  • Description

    Rabbit polyclonal to SREBP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment within Human SREBP2 (internal sequence). The exact sequence is proprietary.
    Database link: Q12772

  • Positive control

    • ICC/IF: HeLa cells. WB: HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab228653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 124 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the cholesterol and to a lesser degree the fatty acid synthesis pathway (By similarity). Binds the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') found in the flanking region of the LDRL and HMG-CoA synthase genes.
  • Tissue specificity

    Ubiquitously expressed in adult and fetal tissues.
  • Sequence similarities

    Belongs to the SREBP family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Post-translational
    modifications

    At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7.
  • Cellular localization

    Nucleus and Endoplasmic reticulum membrane. Golgi apparatus membrane. Cytoplasmic vesicle > COPII-coated vesicle membrane. Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.
  • Information by UniProt
  • Database links

  • Alternative names

    • AI608257 antibody
    • bHLHd2 antibody
    • Class D basic helix-loop-helix protein 2 antibody
    • OTTHUMP00000028740 antibody
    • Processed sterol regulatory element-binding protein 2 antibody
    • SRBP2_HUMAN antibody
    • SREBF 2 antibody
    • Srebf2 antibody
    • SREBF2 protein antibody
    • SREBP 2 antibody
    • SREBP-2 antibody
    • SREBP2 antibody
    • SREBP2gc antibody
    • sterol regulatory element binding factor 2 antibody
    • Sterol regulatory element binding protein 2 antibody
    • Sterol regulatory element binding transcription factor 2 antibody
    • Sterol regulatory element-binding transcription factor 2 antibody
    see all

Images

  • Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for SREBP2 (green) using ab228653 (1/500 dilution) in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue).

  • Anti-SREBP2 antibody (ab228653) at 1/500 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 124 kDa



    7.5% SDS-PAGE

References

ab228653 has not yet been referenced specifically in any publications.

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