Anti-SREBP2 antibody (ab28482)

Reviews (1)Q&A (4)References (16)

Overview

  • Product name

    Anti-SREBP2 antibody
    See all SREBP2 primary antibodies

  • Description

    Rabbit polyclonal to SREBP2

  • Host species

    Rabbit

  • Specificity

    ab28482 recognises Sterol regulatory element-binding protein 2 (SREBP 2)

  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Guinea pig, Cow, Dog, Pig, Chimpanzee

  • Immunogen

    Synthetic peptide corresponding to Rat SREBP2 aa 876-890.
    Sequence:

    AISWLQGDDAAVRSH

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Mouse and rat liver. Rat kidney.

Applications

Our Abpromise guarantee covers the use of ab28482 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Detects a band of approximately 68, 120 kDa (predicted molecular weight: 120 kDa).
ICC/IF 1/20 - 1/200.
IHC-P Use a concentration of 2 µg/ml.

Target

  • Function

    Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the cholesterol and to a lesser degree the fatty acid synthesis pathway (By similarity). Binds the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') found in the flanking region of the LDRL and HMG-CoA synthase genes.

  • Tissue specificity

    Ubiquitously expressed in adult and fetal tissues.

  • Sequence similarities

    Belongs to the SREBP family.
    Contains 1 basic helix-loop-helix (bHLH) domain.

  • Post-translational
    modifications

    At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7.

  • Cellular localization

    Nucleus and Endoplasmic reticulum membrane. Golgi apparatus membrane. Cytoplasmic vesicle > COPII-coated vesicle membrane. Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • AI608257 antibody
    • bHLHd2 antibody
    • Class D basic helix-loop-helix protein 2 antibody
    • OTTHUMP00000028740 antibody
    • Processed sterol regulatory element-binding protein 2 antibody
    • SRBP2_HUMAN antibody
    • SREBF 2 antibody
    • Srebf2 antibody
    • SREBF2 protein antibody
    • SREBP 2 antibody
    • SREBP-2 antibody
    • SREBP2 antibody
    • SREBP2gc antibody
    • sterol regulatory element binding factor 2 antibody
    • Sterol regulatory element binding protein 2 antibody
    • Sterol regulatory element binding transcription factor 2 antibody
    • Sterol regulatory element-binding transcription factor 2 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-SREBP2 antibody (ab28482)
    Immunocytochemistry/ Immunofluorescence - Anti-SREBP2 antibody (ab28482)

    Immunocytochemistry/Immunofluorescent analysis of SREBP2 (green) showing staining in the cytoplasm of PC12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab28482 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • Immunocytochemistry/ Immunofluorescence - Anti-SREBP2 antibody (ab28482)
    Immunocytochemistry/ Immunofluorescence - Anti-SREBP2 antibody (ab28482)

    Immunocytochemistry/Immunofluorescent analysis of SREBP2 (green) showing staining in the cytoplasm of L6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab28482 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • Immunocytochemistry/ Immunofluorescence - Anti-SREBP2 antibody (ab28482)
    Immunocytochemistry/ Immunofluorescence - Anti-SREBP2 antibody (ab28482)

    Immunocytochemistry/Immunofluorescent analysis of SREBP2 (green) showing staining in the cytoplasm of HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab28482 in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SREBP2 antibody (ab28482)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SREBP2 antibody (ab28482)
    ab28482 (2µg/ml) staining SREPB 2 in human temporal cortex using an automated system (DAKO Autostainer Plus). Using this protocol there is staining of the microvasculature, white matter, granule neutrophil and glial cells.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

References

This product has been referenced in:

  • Shi J  et al. Shuangyu Tiaozhi Granule Attenuates Hypercholesterolemia through the Reduction of Cholesterol Synthesis in Rat Fed a High Cholesterol Diet. Biomed Res Int 2019:4805926 (2019). Read more (PubMed: 30937311) »
  • Caron J  et al. Low-density lipoprotein receptor-deficient hepatocytes differentiated from induced pluripotent stem cells allow familial hypercholesterolemia modeling, CRISPR/Cas-mediated genetic correction, and productive hepatitis C virus infection. Stem Cell Res Ther 10:221 (2019). Read more (PubMed: 31358055) »
See all 16 Publications for this product

Publishing research using ab28482? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-5 of 5 Abreviews or Q&A

Question

Yes I would like to proceed with this offer, thank you for suggesting it.

Thanks,

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Answer

Thank you for your response and your interest.

DISCOUNT CODE: 100%ABR-XXXXXX
Expiration date: 26/05/2012

I am very pleased to hear you would like to accept our offer and test ab28482 in Bovine. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview forBovine and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Question

Hello,



I would like to Western blot for SREBP2 in fractions of my bovine cells. Antibody ab28482 is only predicted to react with bovine, would it be possible for you to send a sample or smaller aliquot for me to try out please?



Thanks,

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Answer

Thank you very much for your interest in ab28482.

To our knowledge,ab28482 has not been tested in bovine. Therefore, I can offer a discount off a future purchase if you buyab28482 now, test it inin bovine and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and testab28482 in in bovine. I will then send a discount code. This code must be issued before purchasingab28482 so please wait for my reply before ordering.

2. Purchaseab28482 either by phone, fax, or online (www.abcam.com).

3. Test it in in bovine.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even ifab28482 turns out to be unsuitable for bovine, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Question

To whom may concern, I'd like to know what is the diluent of the ab28482 product (lot 859967). Could it be in ultrapure water or PBS? Best regards.

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Answer

Thank you for contacting us. Indeed, I can confirm that the storage buffer of anti-SREBP2 antibody ab28482 (lot859967) is PBS containing 0.05% sodium azide and 1 mg/ml BSA as a stabilizing protein. This is needed to reduce the risk of degradation by proteases during long term storage. So if you would like to dilute this antibody further, I would recommend using PBS including BSA. In order to dilute the antibody for applications like western Blotting, any suitable buffer like dilution buffer with or without blocking protein (BSA or milk) can be used, as for example PBS or TBS, either with Tween 20 or without. This needs to be determined for the particular experimental setting. I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

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Western blot abreview for Anti-SREBP2 antibody

 Poor
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Hela whole cell lysates)
Loading amount
1 µg
Specification
Hela whole cell lysates
Gel Running Conditions
Reduced Denaturing (4-20%, 15well, 1.5mm)
Blocking step
Milk as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 37°C
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Abcam user community

Verified customer

Submitted Oct 20 2010

Question

Ab28482 was raised to the synthetic peptide AISWLQGDDAAVRSH, corresponding to amino acids 843-857 of Mouse SREBP 2. Depend on this, the n-terminal transcriptional form SREBP 2 will not be recognized by this antibody. why it can recognize both forms of SREBP 2? In our study,i want to find an antibody recognize both forms SREBP2 in rats.

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Answer

Thank you for your enquiry. Regarding ab28482 (SREBP 2), this product does detects an ~68 and 120 kDa protein representing SREBP 2 in mouse and rat liver samples as well as rat kidney samples. A predominent band at ~68 kDa (active cleaved site) is seen and a band at ~120 kDa (inactive precursor) may not be seen or it may be diminished. Yes, the smaller band is the cleaved active site of SREBP 2. Please let me know if I can assist you further.

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