Anti-SRGAP1 antibody [EPR8760(2)] Recombinant (ab171938)

Product name

Anti-SRGAP1 antibody [EPR8760(2)]
See all SRGAP1 primary antibodies
Description

Rabbit monoclonal [EPR8760(2)] to SRGAP1
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
Unsuitable for: IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide within Human SRGAP1 aa 1050 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q7Z6B7
Positive control
- Human fetal brain, Human fetal lung, Human fetal kidney tissue lysates, A549 cells, 293T cell line lysate, Human colon tissue, Human lung tissue.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR8760(2)
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab171938 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/5000. Predicted molecular weight: 124 kDa.
IHC-P		1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF		1/100 - 1/250.
Flow Cyt		1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Application notes

Is unsuitable for IP.

Function

GTPase-activating protein for RhoA and Cdc42 small GTPases. Together with CDC42 seems to be involved in the pathway mediating the repulsive signaling of Robo and Slit proteins in neuronal migration. SLIT2, probably through interaction with ROBO1, increases the interaction of SRGAP1 with ROBO1 and inactivates CDC42.
Tissue specificity

Expressed in brain, lung, kidney, and testis.
Sequence similarities

Contains 1 FCH domain.
Contains 1 Rho-GAP domain.
Contains 1 SH3 domain.
Domain

The F-BAR domain mediates oligomerization, binds membranes, and constrains plasma membrane protrusions.
Target information above from: UniProt accession Q7Z6B7 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 57522 Human
- Entrez Gene: 117600 Mouse
- Entrez Gene: 314903 Rat
- Omim: 606523 Human
- SwissProt: Q7Z6B7 Human
- SwissProt: Q91Z69 Mouse
- Unigene: 210751 Human
- Unigene: 593803 Human
- Unigene: 288698 Mouse
- Unigene: 208903 Rat
see all
Alternative names
- 4930572H05Rik antibody
- ARHGAP13 antibody
- FLJ22166 antibody
- KIAA1304 antibody
- RGD1566260 antibody
- Rho GTPase activating protein 13 antibody
- Rho GTPase-activating protein 13 antibody
- SLIT ROBO Rho GTPase activating protein 1 antibody
- SLIT-ROBO Rho GTPase-activating protein 1 antibody
- SRGAP 1 antibody
- srGAP1 antibody
- SRGP1_HUMAN antibody
see all

Flow Cytometry - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)

Flow cytometric analysis of permeabilized 293T cells labeling SRGAP1 with ab171938 at 1/100 (red) or a rabbit IgG (negative) (green).
Immunocytochemistry/ Immunofluorescence - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)

Immunofluorescent analysis of A549 cells labeling SRGAP1 with ab171938 at 1/100 (green). DAPI nuclear staining (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)

Immunohistochemical analysis of paraffin embedded Human lung tissue labeling SRGAP1 with ab171938 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)

Immunohistochemical analysis of paraffin embedded Human colon tissue labeling SRGAP1 with ab171938 at 1/100 dilution.
Western blot - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)

All lanes : Anti-SRGAP1 antibody [EPR8760(2)] (ab171938) at 1/1000 dilution

Lane 1 : Human fetal brain tissue lysate
Lane 2 : 293T cell line lysate
Lane 3 : A549 cell line lysate
Lane 4 : Human fetal lung tissue lysate
Lane 5 : Human fetal kidney tissue lysate

Lysates/proteins at 10 µg per lane.

Developed using the ECL technique.

Predicted band size: 124 kDa

ab171938 has not yet been referenced specifically in any publications.

Publishing research using ab171938? Please let us know so that we can cite the reference in this datasheet.

Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)