Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)
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- References
- Protocols
Overview
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Product nameAnti-SRGAP1 antibody [EPR8760(2)]
See all SRGAP1 primary antibodies -
DescriptionRabbit monoclonal [EPR8760(2)] to SRGAP1
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
Unsuitable for: IP -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human SRGAP1 aa 1050 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q7Z6B7 -
Positive control
- Human fetal brain, Human fetal lung, Human fetal kidney tissue lysates, A549 cells, 293T cell line lysate, Human colon tissue, Human lung tissue.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR8760(2)
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IsotypeIgG
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Research areas
Associated products
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab171938 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 124 kDa. | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/100 - 1/250. | |
| Flow Cyt | 1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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FunctionGTPase-activating protein for RhoA and Cdc42 small GTPases. Together with CDC42 seems to be involved in the pathway mediating the repulsive signaling of Robo and Slit proteins in neuronal migration. SLIT2, probably through interaction with ROBO1, increases the interaction of SRGAP1 with ROBO1 and inactivates CDC42.
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Tissue specificityExpressed in brain, lung, kidney, and testis.
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Sequence similaritiesContains 1 FCH domain.
Contains 1 Rho-GAP domain.
Contains 1 SH3 domain. -
DomainThe F-BAR domain mediates oligomerization, binds membranes, and constrains plasma membrane protrusions.
- Information by UniProt
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Database links
- Entrez Gene: 57522 Human
- Entrez Gene: 117600 Mouse
- Entrez Gene: 314903 Rat
- Omim: 606523 Human
- SwissProt: Q7Z6B7 Human
- SwissProt: Q91Z69 Mouse
- Unigene: 210751 Human
- Unigene: 593803 Human
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Alternative names
- 4930572H05Rik antibody
- ARHGAP13 antibody
- FLJ22166 antibody
see all
Images
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![Western blot - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)](https://www.abcam.com/ps/products/171/ab171938/Images/ab171938-193568-ab171938WB.jpg)
Western blot - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)All lanes : Anti-SRGAP1 antibody [EPR8760(2)] (ab171938) at 1/1000 dilution
Lane 1 : Human fetal brain tissue lysate
Lane 2 : 293T cell line lysate
Lane 3 : A549 cell line lysate
Lane 4 : Human fetal lung tissue lysate
Lane 5 : Human fetal kidney tissue lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 124 kDa -
![Flow Cytometry - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)](https://www.abcam.com/ps/products/171/ab171938/Images/ab171938-193578-ab171938FC.jpg)
Flow Cytometry - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)Flow cytometric analysis of permeabilized 293T cells labeling SRGAP1 with ab171938 at 1/100 (red) or a rabbit IgG (negative) (green).
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![Immunocytochemistry/ Immunofluorescence - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)](https://www.abcam.com/ps/products/171/ab171938/Images/ab171938-193575-ab171938IF.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)Immunofluorescent analysis of A549 cells labeling SRGAP1 with ab171938 at 1/100 (green). DAPI nuclear staining (blue).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)](https://www.abcam.com/ps/products/171/ab171938/Images/ab171938-193573-ab171938IHC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)Immunohistochemical analysis of paraffin embedded Human lung tissue labeling SRGAP1 with ab171938 at 1/100 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)](https://www.abcam.com/ps/products/171/ab171938/Images/ab171938-193571-ab171938IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)Immunohistochemical analysis of paraffin embedded Human colon tissue labeling SRGAP1 with ab171938 at 1/100 dilution.
Protocols
Datasheets and documents
References
ab171938 has not yet been referenced specifically in any publications.
