RecombinantRabMAb

Recombinant Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)

Submit a review Submit a question
Western blot - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)
  • Flow Cytometry - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)
  • Immunocytochemistry/ Immunofluorescence - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)
  • Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8760(2)] to SRGAP1
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-SRGAP1 antibody [EPR8760(2)]
    See all SRGAP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR8760(2)] to SRGAP1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human SRGAP1 aa 1050 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q7Z6B7

  • Positive control

    • Human fetal brain, Human fetal lung, Human fetal kidney tissue lysates, A549 cells, 293T cell line lysate, Human colon tissue, Human lung tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab171938 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC/IF
Human
IHC-P
Human
WB
Human
All applications
Mouse
Rat
Application Abreviews Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 124 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
Flow Cyt
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 124 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
Flow Cyt
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function

      GTPase-activating protein for RhoA and Cdc42 small GTPases. Together with CDC42 seems to be involved in the pathway mediating the repulsive signaling of Robo and Slit proteins in neuronal migration. SLIT2, probably through interaction with ROBO1, increases the interaction of SRGAP1 with ROBO1 and inactivates CDC42.

    • Tissue specificity

      Expressed in brain, lung, kidney, and testis.

    • Sequence similarities

      Contains 1 FCH domain.
      Contains 1 Rho-GAP domain.
      Contains 1 SH3 domain.

    • Domain

      The F-BAR domain mediates oligomerization, binds membranes, and constrains plasma membrane protrusions.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • 4930572H05Rik antibody
      • ARHGAP13 antibody
      • FLJ22166 antibody
      • KIAA1304 antibody
      • RGD1566260 antibody
      • Rho GTPase activating protein 13 antibody
      • Rho GTPase-activating protein 13 antibody
      • SLIT ROBO Rho GTPase activating protein 1 antibody
      • SLIT-ROBO Rho GTPase-activating protein 1 antibody
      • SRGAP 1 antibody
      • srGAP1 antibody
      • SRGP1_HUMAN antibody
      see all

    Images

    • Western blot - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)
      Western blot - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)
      All lanes : Anti-SRGAP1 antibody [EPR8760(2)] (ab171938) at 1/1000 dilution

      Lane 1 : Human fetal brain tissue lysate
      Lane 2 : 293T cell line lysate
      Lane 3 : A549 cell line lysate
      Lane 4 : Human fetal lung tissue lysate
      Lane 5 : Human fetal kidney tissue lysate

      Lysates/proteins at 10 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 124 kDa

    • Flow Cytometry - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)
      Flow Cytometry - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)

      Flow cytometric analysis of permeabilized 293T cells labeling SRGAP1 with ab171938 at 1/100 (red) or a rabbit IgG (negative) (green).

    • Immunocytochemistry/ Immunofluorescence - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)
      Immunocytochemistry/ Immunofluorescence - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)

      Immunofluorescent analysis of A549 cells labeling SRGAP1 with ab171938 at 1/100 (green). DAPI nuclear staining (blue).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)

      Immunohistochemical analysis of paraffin embedded Human lung tissue labeling SRGAP1 with ab171938 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)

      Immunohistochemical analysis of paraffin embedded Human colon tissue labeling SRGAP1 with ab171938 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)
      Anti-SRGAP1 antibody [EPR8760(2)] (ab171938)

    References (0)

    Publishing research using ab171938? Please let us know so that we can cite the reference in this datasheet.

    ab171938 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab171938.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com