Anti-srGAP2 antibody (ab121977)
Key features and details
- Rabbit polyclonal to srGAP2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-srGAP2 antibody
See all srGAP2 primary antibodies -
Description
Rabbit polyclonal to srGAP2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human, African green monkey -
Immunogen
antigen: LEPLKTSPVV APTSEPSSPL HTQLLKDPEP AFQRSASTAG DIACAFRPVK SVKMAAPVKP PATRPKPTVF PKTNATSPGV NSSTSPQST, corresponding to amino acids 977-1065 of Human srGAP2.
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General notes
ab121977 is mono-specific.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Primary antibody notes
ab121977 is mono-specific. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121977 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 121 kDa.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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Notes |
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IHC-P
1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 121 kDa. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
Target
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Function
Putative GTPase-activating protein for Rho family small GTPases. -
Tissue specificity
Expression at low levels in placenta, kidney, and ovary, and little or no expression in all other tissues tested. -
Sequence similarities
Contains 1 FCH domain.
Contains 1 Rho-GAP domain.
Contains 1 SH3 domain. - Information by UniProt
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Database links
- Entrez Gene: 23380 Human
- Entrez Gene: 14270 Mouse
- Omim: 606524 Human
- SwissProt: O75044 Human
- SwissProt: Q91Z67 Mouse
- Unigene: 497575 Human
- Unigene: 276259 Mouse
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Alternative names
- ARHGAP34 antibody
- FBP2 antibody
- FNBP2 antibody
see all
Images
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Immunohistochemical analysis of human bone marrow labelling SRGAP2 using ab121977 at 1/50 dilution. Positive nuclear and cytoplasmic staining observed in hematopoietic cells.
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Immunohistochemical analysis of human testis tissue labelling SRGAP2 using ab121977 at 1/50 dilution. Positive nuclear and cytoplasmic staining observed in Leydig cells and seminiferous duct cells.
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Immunohistochemical analysis of human kidney tissue labelling SRGAP2 using ab121977 at 1/50 dilution. Positive nuclear and cytoplasmic staining observed in distal tube cells.
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All lanes : Anti-srGAP2 antibody (ab121977) at 1/100 dilution
Lane 1 : COS7 Whole cell lysate
Lane 2 : HEK293H Whole Cell Lysate
Lane 3 : HEK293H Over expressing human srGAP1
Lane 4 : HEK293H Over expressing human srGAP2
Lane 5 : HEK293H Over expressing human srGAP3
Lane 6 : HeLa cells over expressing mouse srGAP2
Lane 7 : Mouse E14 hindbrain lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG Conjugated to HRP at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 121 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Additional bands at: 200 kDa (possible tagged protein)
Exposure time: 5 minutesBlocking: 5% MIlk
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Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121977 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab121977 has been referenced in 3 publications.
- Jin D et al. Impact of klotho on the expression of SRGAP2a in podocytes in diabetic nephropathy. BMC Nephrol 23:151 (2022). PubMed: 35436879
- Pan Y et al. Dissection of Glomerular Transcriptional Profile in Patients With Diabetic Nephropathy: SRGAP2a Protects Podocyte Structure and Function. Diabetes 67:717-730 (2018). PubMed: 29242313
- Marko TA et al. Slit-Robo GTPase-Activating Protein 2 as a metastasis suppressor in osteosarcoma. Sci Rep 6:39059 (2016). PubMed: 27966608