Key features and details
- Rabbit polyclonal to srGAP2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-srGAP2 antibody
See all srGAP2 primary antibodies
DescriptionRabbit polyclonal to srGAP2
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
ab121977 is mono-specific.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS
Concentration information loading...
PurityImmunogen affinity purified
Primary antibody notesab121977 is mono-specific.
Our Abpromise guarantee covers the use of ab121977 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 121 kDa.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
FunctionPutative GTPase-activating protein for Rho family small GTPases.
Tissue specificityExpression at low levels in placenta, kidney, and ovary, and little or no expression in all other tissues tested.
Sequence similaritiesContains 1 FCH domain.
Contains 1 Rho-GAP domain.
Contains 1 SH3 domain.
- Information by UniProt
- ARHGAP34 antibody
- FBP2 antibody
- FNBP2 antibody
All lanes : Anti-srGAP2 antibody (ab121977) at 1/100 dilution
Lane 1 : COS7 Whole cell lysate
Lane 2 : HEK293H Whole Cell Lysate
Lane 3 : HEK293H Over expressing human srGAP1
Lane 4 : HEK293H Over expressing human srGAP2
Lane 5 : HEK293H Over expressing human srGAP3
Lane 6 : HeLa cells over expressing mouse srGAP2
Lane 7 : Mouse E14 hindbrain lysate
Lysates/proteins at 30 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG Conjugated to HRP at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 121 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Additional bands at: 200 kDa (possible tagged protein)
Exposure time: 5 minutes
Blocking: 5% MIlk
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121977 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab121977, at 1/10 dilution, staining srGAP2 in paraffin-embedded Human adrenal gland tissue by Immunohistochemistry.
ab121977 has been referenced in 2 publications.
- Pan Y et al. Dissection of Glomerular Transcriptional Profile in Patients With Diabetic Nephropathy: SRGAP2a Protects Podocyte Structure and Function. Diabetes 67:717-730 (2018). PubMed: 29242313
- Marko TA et al. Slit-Robo GTPase-Activating Protein 2 as a metastasis suppressor in osteosarcoma. Sci Rep 6:39059 (2016). PubMed: 27966608