Product nameAnti-srGAP2 antibody
See all srGAP2 primary antibodies
DescriptionRabbit polyclonal to srGAP2
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
- Human adrenal gland tissue.
ab121977 is mono-specific.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Primary antibody notesab121977 is mono-specific.
Our Abpromise guarantee covers the use of ab121977 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/100. Detects a band of approximately 170 kDa (predicted molecular weight: 121 kDa).
Abcam recommends 5% milk blocking
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
FunctionPutative GTPase-activating protein for Rho family small GTPases.
Tissue specificityExpression at low levels in placenta, kidney, and ovary, and little or no expression in all other tissues tested.
Sequence similaritiesContains 1 FCH domain.
Contains 1 Rho-GAP domain.
Contains 1 SH3 domain.
- Information by UniProt
- ARHGAP34 antibody
- FBP2 antibody
- FNBP2 antibody
All lanes : Anti-srGAP2 antibody (ab121977) at 1/100 dilution
Lane 1 : COS7 Whole cell lysate
Lane 2 : HEK293H Whole Cell Lysate
Lane 3 : HEK293H Over expressing human srGAP1
Lane 4 : HEK293H Over expressing human srGAP2
Lane 5 : HEK293H Over expressing human srGAP3
Lane 6 : HeLa cells over expressing mouse srGAP2
Lane 7 : Mouse E14 hindbrain lysate
Lysates/proteins at 30 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG Conjugated to HRP at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 121 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Additional bands at: 200 kDa (possible tagged protein)
Exposure time: 5 minutes
Blocking: 5% MIlk
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121977 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab121977, at 1/10 dilution, staining srGAP2 in paraffin-embedded Human adrenal gland tissue by Immunohistochemistry.