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Rabbit polyclonal to srGAP2
antigen: LEPLKTSPVV APTSEPSSPL HTQLLKDPEP AFQRSASTAG DIACAFRPVK SVKMAAPVKP PATRPKPTVF PKTNATSPGV NSSTSPQST, corresponding to amino acids 977-1065 of Human srGAP2.
Human adrenal gland tissue.
ab121977 is mono-specific.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
Primary antibody notes
ab121977 is mono-specific.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/100. Detects a band of approximately 170 kDa (predicted molecular weight: 121 kDa).
Abcam recommends 5% milk blocking
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
Putative GTPase-activating protein for Rho family small GTPases.
Expression at low levels in placenta, kidney, and ovary, and little or no expression in all other tissues tested.
Contains 1 FCH domain.
Contains 1 Rho-GAP domain. Contains 1 SH3 domain.
Information by UniProt
Immunocytochemistry/ Immunofluorescence - Anti-srGAP2 antibody (ab121977)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121977 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-srGAP2 antibody (ab121977) This image is courtesy of Alain Chedotal (Institut Vision, France)
All lanes :
Anti-srGAP2 antibody (ab121977) at 1/100 dilution
Lane 1 :
COS7 Whole cell lysate
Lane 2 :
HEK293H Whole Cell Lysate
Lane 3 :
HEK293H Over expressing human srGAP1
Lane 4 :
HEK293H Over expressing human srGAP2
Lane 5 :
HEK293H Over expressing human srGAP3
Lane 6 :
HeLa cells over expressing mouse srGAP2
Lane 7 :
Mouse E14 hindbrain lysate
Lysates/proteins at 30 µg per lane.
Secondary All lanes :
Goat polyclonal to Rabbit IgG Conjugated to HRP at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
170 kDa (
why is the actual band size different from the predicted?
Additional bands at:
200 kDa (possible tagged protein)
Blocking: 5% MIlk
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-srGAP2 antibody (ab121977)
ab121977, at 1/10 dilution, staining srGAP2 in paraffin-embedded Human adrenal gland tissue by Immunohistochemistry.
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