Anti-SRGAP2B antibody (ab222902)
Key features and details
- Rabbit polyclonal to SRGAP2B
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SRGAP2B antibody -
Description
Rabbit polyclonal to SRGAP2B -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human SRGAP2B aa 50-150.
Database link: P0DMP2 -
Positive control
- WB: Mouse heart tissue lysate. ICC/IF: MCF7 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab222902 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Detects a band of approximately 54 kDa (predicted molecular weight: 53 kDa).
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ICC/IF |
1/50 - 1/200.
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Notes |
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WB
1/1000 - 1/5000. Detects a band of approximately 54 kDa (predicted molecular weight: 53 kDa). |
ICC/IF
1/50 - 1/200. |
Target
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Function
May regulate cell migration and differentiation through interaction with and inhibition of SRGAP2. -
Sequence similarities
Contains 1 F-BAR domain. - Information by UniProt
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Database links
- Entrez Gene: 647135 Human
- SwissProt: P0DMP2 Human
- Unigene: 497575 Human
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Alternative names
- SLIT-ROBO Rho GTPase activating protein 2 pseudogene 2 antibody
- SLIT-ROBO Rho GTPase-activating protein 2B antibody
- SRG2B_HUMAN antibody
- SRGAP2B antibody
Images
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Anti-SRGAP2B antibody (ab222902) at 1/1000 dilution + mouse heart tissue lysate
Secondary
Goat polyclonal to rabbit IgG at 1/500000 dilution
Predicted band size: 53 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted? -
MCF7 (human breast adenocarcinoma cell line) cells stained for SRGAP2B (green) using ab222902 (1/100 dilution) in ICC/IF. Secondary: Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab222902 has not yet been referenced specifically in any publications.