Key features and details
- Rabbit polyclonal to SRGAP2B
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-SRGAP2B antibody
DescriptionRabbit polyclonal to SRGAP2B
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
- WB: Mouse heart tissue lysate. ICC/IF: MCF7 cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab222902 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 54 kDa (predicted molecular weight: 53 kDa).|
|ICC/IF||1/50 - 1/200.|
FunctionMay regulate cell migration and differentiation through interaction with and inhibition of SRGAP2.
Sequence similaritiesContains 1 F-BAR domain.
- Information by UniProt
- SLIT-ROBO Rho GTPase activating protein 2 pseudogene 2 antibody
- SLIT-ROBO Rho GTPase-activating protein 2B antibody
- SRG2B_HUMAN antibody
- SRGAP2B antibody
Anti-SRGAP2B antibody (ab222902) at 1/1000 dilution + mouse heart tissue lysate
Goat polyclonal to rabbit IgG at 1/500000 dilution
Predicted band size: 53 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?
MCF7 (human breast adenocarcinoma cell line) cells stained for SRGAP2B (green) using ab222902 (1/100 dilution) in ICC/IF. Secondary: Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
ab222902 has not yet been referenced specifically in any publications.