Overview

  • Product name

    Anti-SRGAP2B antibody
  • Description

    Rabbit polyclonal to SRGAP2B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human SRGAP2B aa 79-105.
    Sequence:

    CSTKDQQFKKDQNVLSPVNCWNLLLNQ


    Database link: P0DMP2

  • Positive control

    • WB: Mouse heart tissue lysate. ICC/IF: MCF7 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.03% Proclin
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purity >95%
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab222902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 54 kDa (predicted molecular weight: 53 kDa).
ICC/IF 1/50 - 1/200.

Target

  • Function

    May regulate cell migration and differentiation through interaction with and inhibition of SRGAP2.
  • Sequence similarities

    Contains 1 F-BAR domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • SLIT-ROBO Rho GTPase activating protein 2 pseudogene 2 antibody
    • SLIT-ROBO Rho GTPase-activating protein 2B antibody
    • SRG2B_HUMAN antibody
    • SRGAP2B antibody

Images

  • Anti-SRGAP2B antibody (ab222902) at 1/1000 dilution + mouse heart tissue lysate

    Secondary
    Goat polyclonal to rabbit IgG at 1/500000 dilution

    Predicted band size: 53 kDa
    Observed band size: 54 kDa
    why is the actual band size different from the predicted?

  • MCF7 (human breast adenocarcinoma cell line) cells stained for SRGAP2B (green) using ab222902 (1/100 dilution) in ICC/IF. Secondary: Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

References

ab222902 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab222902.
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