Key features and details
- Rabbit polyclonal to SRP1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SRP1 antibody
DescriptionRabbit polyclonal to SRP1
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Orangutan
Recombinant fragment corresponding to Human SRP1 aa 8-538.
NFRLKSYKNKSLNPDEMRRRREEEGLQLRKQKREEQLFKRRNVATAEEET EEEVMSDGGFHEAQISNMEMAPGGVITSDMIEMIFSKSPEQQLSATQKFR KLLSKEPNPPIDEVISTPGVVARFVEFLKRKENCTLQFESAWVLTNIASG NSLQTRIVIQAGAVPIFIELLSSEFEDVQEQAVWALGNIAGDSTMCRDYV LDCNILPPLLQLFSKQNRLTMTRNAVWALSNLCRGKSPPPEFAKVSPCLN VLSWLLFVSDTDVLADACWALSYLSDGPNDKIQAVIDAGVCRRLVELLMH NDYKVVSPALRAVGNIVTGDDIQTQVILNCSALQSLLHLLSSPKESIKKE ACWTISNITAGNRAQIQTVIDANIFPALISILQTAEFRTRKEAAWAITNA TSGGSAEQIKYLVELGCIKPLCDLLTVMDSKIVQVALNGLENILRLGEQE AKRNGTGINPYCALIEEAYGLDKIEFLQSHENQEIYQKAFDLIEHYFGTE DEDSSIAPQVDLNQQQYIFQQCEAPMEGFQL
Database link: P52294
- IHC-P: Human lung cancer and tonsil tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab238599 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
FunctionFunctions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.
Tissue specificityExpressed ubiquitously.
Sequence similaritiesBelongs to the importin alpha family.
Contains 10 ARM repeats.
Contains 1 IBB domain.
DomainConsists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import.
The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins.
The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding.
modificationsPolyubiquitinated in the presence of RAG1 (in vitro).
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- IMA5_HUMAN antibody
- Importin alpha 1 subunit antibody
- Importin alpha 5 antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab238599 has not yet been referenced specifically in any publications.