Recombinant Anti-SRP54 antibody [EPR10850] (ab155964)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-SRP54 antibody [EPR10850]
See all SRP54 primary antibodies -
Description
Rabbit monoclonal [EPR10850] to SRP54 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to residues in Human SRP54 (P61011)
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Positive control
- Recombinant Human SRP54 protein (ab116873) can be used as a positive control in WB. 293T, HeLa, Daudi or Jurkat cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10850 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab155964 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 56 kDa. |
Target
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Function
Binds to the signal sequence of presecretory protein when they emerge from the ribosomes and transfers them to TRAM (translocating chain-associating membrane protein). -
Sequence similarities
Belongs to the GTP-binding SRP family. -
Domain
Has a two domain structure: the G-domain binds GTP; the M-domain binds the 7S RNA in presence of SRP19 and also binds the signal sequence. -
Cellular localization
Nucleus speckle. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 6729 Human
- Entrez Gene: 24067 Mouse
- Entrez Gene: 665155 Mouse
- Entrez Gene: 116650 Rat
- Omim: 604857 Human
- SwissProt: P61011 Human
- SwissProt: P14576 Mouse
- SwissProt: Q6AYB5 Rat
see all -
Alternative names
- Signal recognition particle 54 kDa protein antibody
- Signal recognition particle 54kDa antibody
- SRP 54 antibody
see all
Images
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![Western blot - Anti-SRP54 antibody [EPR10850] (ab155964)](https://www.abcam.com/ps/products/155/ab155964/Images/ab155964-163258-anti-srp54-antibody-epr10850-western-blot.jpg)
Western blot - Anti-SRP54 antibody [EPR10850] (ab155964)All lanes : Anti-SRP54 antibody [EPR10850] (ab155964) at 1/1000 dilution
Lane 1 : 293T lysate
Lane 2 : HeLa lysate
Lane 3 : Daudi lysate
Lane 4 : Jurkat lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 56 kDa
Protocols
References
ab155964 has not yet been referenced specifically in any publications.
