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Signal Transduction Protein Trafficking ER Proteins
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RecombinantRabMAb

Recombinant Anti-SRP54 antibody [EPR10850] (ab155964)

  • Datasheet
  • SDS
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Western blot - Anti-SRP54 antibody [EPR10850] (ab155964)
  • Anti-SRP54 antibody [EPR10850] (ab155964)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10850] to SRP54
  • Suitable for: WB
  • Reacts with: Human

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-SRP54 antibody [EPR10850]
    See all SRP54 primary antibodies
  • Description

    Rabbit monoclonal [EPR10850] to SRP54
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to residues in Human SRP54 (P61011)

  • Positive control

    • Recombinant Human SRP54 protein (ab116873) can be used as a positive control in WB. 293T, HeLa, Daudi or Jurkat cell lysates.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR10850
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • ER Proteins

Associated products

  • Alternative Versions

    • Anti-SRP54 antibody [EPR10850] - BSA and Azide free (ab249217)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • Daudi whole cell lysate (ab3951)
    • Jurkat whole cell lysate (ab7899)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab155964 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      Binds to the signal sequence of presecretory protein when they emerge from the ribosomes and transfers them to TRAM (translocating chain-associating membrane protein).
    • Sequence similarities

      Belongs to the GTP-binding SRP family.
    • Domain

      Has a two domain structure: the G-domain binds GTP; the M-domain binds the 7S RNA in presence of SRP19 and also binds the signal sequence.
    • Cellular localization

      Nucleus speckle. Cytoplasm.
    • Target information above from: UniProt accession P61011 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 6729 Human
      • Entrez Gene: 24067 Mouse
      • Entrez Gene: 665155 Mouse
      • Entrez Gene: 116650 Rat
      • Omim: 604857 Human
      • SwissProt: P61011 Human
      • SwissProt: P14576 Mouse
      • SwissProt: Q6AYB5 Rat
      • Unigene: 167535 Human
      • Unigene: 12848 Mouse
      • Unigene: 471968 Mouse
      • Unigene: 24880 Rat
      see all
    • Alternative names

      • Signal recognition particle 54 kDa protein antibody
      • Signal recognition particle 54kDa antibody
      • SRP 54 antibody
      • SRP54 antibody
      • SRP54_HUMAN antibody
      see all

    Images

    • Western blot - Anti-SRP54 antibody [EPR10850] (ab155964)
      Western blot - Anti-SRP54 antibody [EPR10850] (ab155964)
      All lanes : Anti-SRP54 antibody [EPR10850] (ab155964) at 1/1000 dilution

      Lane 1 : 293T lysate
      Lane 2 : HeLa lysate
      Lane 3 : Daudi lysate
      Lane 4 : Jurkat lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 56 kDa

    • Anti-SRP54 antibody [EPR10850] (ab155964)
      Anti-SRP54 antibody [EPR10850] (ab155964)

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab155964? Please let us know so that we can cite the reference in this datasheet.

    ab155964 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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