Anti-SRP54 antibody [EPR10850] (ab155964)
- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-SRP54 antibody [EPR10850]
See all SRP54 primary antibodies -
DescriptionRabbit monoclonal [EPR10850] to SRP54
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Host speciesRabbit
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Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide corresponding to residues in Human SRP54 (P61011)
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Positive control
- 293T, HeLa, Daudi or Jurkat cell lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR10850
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IsotypeIgG
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Research areas
Associated products
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab155964 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 56 kDa. |
Target
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FunctionBinds to the signal sequence of presecretory protein when they emerge from the ribosomes and transfers them to TRAM (translocating chain-associating membrane protein).
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Sequence similaritiesBelongs to the GTP-binding SRP family.
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DomainHas a two domain structure: the G-domain binds GTP; the M-domain binds the 7S RNA in presence of SRP19 and also binds the signal sequence.
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Cellular localizationNucleus speckle. Cytoplasm.
- Information by UniProt
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Database links
- Entrez Gene: 6729 Human
- Entrez Gene: 24067 Mouse
- Entrez Gene: 665155 Mouse
- Entrez Gene: 116650 Rat
- Omim: 604857 Human
- SwissProt: P61011 Human
- SwissProt: P14576 Mouse
- SwissProt: Q6AYB5 Rat
see all -
Alternative names
- Signal recognition particle 54 kDa protein antibody
- Signal recognition particle 54kDa antibody
- SRP 54 antibody
see all
Images
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![Western blot - Anti-SRP54 antibody [EPR10850] (ab155964)](https://www.abcam.com/ps/datasheet/images/155/ab155964/SRP54-Primary-antibodies-ab155964-1.jpg)
Western blot - Anti-SRP54 antibody [EPR10850] (ab155964)All lanes : Anti-SRP54 antibody [EPR10850] (ab155964) at 1/1000 dilution
Lane 1 : 293T lysate
Lane 2 : HeLa lysate
Lane 3 : Daudi lysate
Lane 4 : Jurkat lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 56 kDa
Protocols
References
ab155964 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"