Overview

  • Product name
    Anti-SRP54 antibody [EPR10851(B)]
    See all SRP54 primary antibodies
  • Description
    Rabbit monoclonal [EPR10851(B)] to SRP54
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, IP, Flow Cytmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SRP54. The exact sequence is proprietary.

  • Positive control
    • Human placenta, fetal brain, 293T, Jurkat and HeLa lysates; Human brain and breast carcinoma tissues.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20ºC.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR10851(B)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    Images

    • All lanes : Anti-SRP54 antibody [EPR10851(B)] (ab154796) at 1/1000 dilution

      Lane 1 : Human placenta lysate
      Lane 2 : fetal brain lysate
      Lane 3 : 293T lysate
      Lane 4 : Jurkat lysate
      Lane 5 : HeLa lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 56 kDa

    • Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling SRP54 with ab154796 at 1/100 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling SRP54 with ab154796 at 1/100 dilution.
    • Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154796 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human normal pancreas tissue using ab154796 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue using ab154796 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue using ab154796 showing +ve staining.

    References

    ab154796 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    ChIP
    Sample
    Rat Cell lysate - whole cell (BRAIN)
    Specification
    BRAIN
    Detection step
    Other
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 15 minute(s) and 0 second(s)

    Abcam user community

    Verified customer

    Submitted Apr 30 2015

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% Triton X-100 in PBS

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Feb 21 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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