Product nameAnti-SRP54 antibody [EPR10851(B)]
See all SRP54 primary antibodies
DescriptionRabbit monoclonal [EPR10851(B)] to SRP54
Tested applicationsSuitable for: WB, IHC-P, IP, Flow Cytmore details
Unsuitable for: ICC/IF
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human SRP54. The exact sequence is proprietary.
- Human placenta, fetal brain, 293T, Jurkat and HeLa lysates; Human brain and breast carcinoma tissues.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab154796 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 56 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IP||1/10 - 1/100.|
|Flow Cyt||1/10 - 1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionBinds to the signal sequence of presecretory protein when they emerge from the ribosomes and transfers them to TRAM (translocating chain-associating membrane protein).
Sequence similaritiesBelongs to the GTP-binding SRP family.
DomainHas a two domain structure: the G-domain binds GTP; the M-domain binds the 7S RNA in presence of SRP19 and also binds the signal sequence.
Cellular localizationNucleus speckle. Cytoplasm.
- Information by UniProt
- Signal recognition particle 54 kDa protein antibody
- Signal recognition particle 54kDa antibody
- SRP 54 antibody
All lanes : Anti-SRP54 antibody [EPR10851(B)] (ab154796) at 1/1000 dilution
Lane 1 : Human placenta lysate
Lane 2 : fetal brain lysate
Lane 3 : 293T lysate
Lane 4 : Jurkat lysate
Lane 5 : HeLa lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 56 kDa
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling SRP54 with ab154796 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling SRP54 with ab154796 at 1/100 dilution.
Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154796 showing +ve staining.
Immunohistochemical analysis of paraffin embedded Human normal pancreas tissue using ab154796 showing +ve staining.
Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue using ab154796 showing +ve staining.
Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue using ab154796 showing +ve staining.
ab154796 has not yet been referenced specifically in any publications.