Recombinant Anti-SRP54 antibody [EPR10851(B)] (ab154796)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10851(B)] to SRP54
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-SRP54 antibody [EPR10851(B)]
See all SRP54 primary antibodies -
Description
Rabbit monoclonal [EPR10851(B)] to SRP54 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human SRP54. The exact sequence is proprietary.
-
Positive control
- Human placenta, fetal brain, 293T, Jurkat and HeLa lysates; Human brain and breast carcinoma tissues.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10851(B) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154796 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
|
|
IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
Notes |
---|
WB
1/1000 - 1/10000. Predicted molecular weight: 56 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
-
Function
Binds to the signal sequence of presecretory protein when they emerge from the ribosomes and transfers them to TRAM (translocating chain-associating membrane protein). -
Sequence similarities
Belongs to the GTP-binding SRP family. -
Domain
Has a two domain structure: the G-domain binds GTP; the M-domain binds the 7S RNA in presence of SRP19 and also binds the signal sequence. -
Cellular localization
Nucleus speckle. Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 6729 Human
- Entrez Gene: 24067 Mouse
- Entrez Gene: 665155 Mouse
- Entrez Gene: 116650 Rat
- Omim: 604857 Human
- SwissProt: P61011 Human
- SwissProt: P14576 Mouse
- SwissProt: Q6AYB5 Rat
see all -
Alternative names
- Signal recognition particle 54 kDa protein antibody
- Signal recognition particle 54kDa antibody
- SRP 54 antibody
see all
Images
-
All lanes : Anti-SRP54 antibody [EPR10851(B)] (ab154796) at 1/1000 dilution
Lane 1 : Human placenta lysate
Lane 2 : fetal brain lysate
Lane 3 : 293T lysate
Lane 4 : Jurkat lysate
Lane 5 : HeLa lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 56 kDa -
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling SRP54 with ab154796 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling SRP54 with ab154796 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154796 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin embedded Human normal pancreas tissue using ab154796 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue using ab154796 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue using ab154796 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab154796 has been referenced in 1 publication.
- Zhao Y et al. Intragenic Transcriptional cis-Antagonism Across SLC6A3. Mol Neurobiol 56:4051-4060 (2019). PubMed: 30259411