Overview

  • Product name

    Anti-SRP72 antibody [EPR17143]
    See all SRP72 primary antibodies
  • Description

    Rabbit monoclonal [EPR17143] to SRP72
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SRP72 aa 600 to the C-terminus. The exact sequence is proprietary.
    Database link: O76094

  • Positive control

    • WB: K562, HeLa and Jurkat cell lysates; Human fetal brain lysate. ICC/IF: HeLa and Jurkat cells. Flow Cyt: Jurkat cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab200199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
ICC/IF 1/100.

Target

  • Function

    Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. Binds the 7S RNA only in presence of SRP68. This ribonucleoprotein complex might interact directly with the docking protein in the ER membrane and possibly participate in the elongation arrest function.
  • Involvement in disease

    Bone marrow failure, familial
  • Sequence similarities

    Belongs to the SRP72 family.
    Contains 5 TPR repeats.
  • Domain

    The C-terminus is essential for the interaction with the SRP68/7S RNA complex.
  • Cellular localization

    Cytoplasm. Endoplasmic reticulum.
  • Information by UniProt
  • Database links

  • Alternative names

    • BMFF antibody
    • BMFS1 antibody
    • Epididymis luminal protein 103 antibody
    • HEL103 antibody
    • Signal recognition particle 72 kDa protein antibody
    • Signal recognition particle 72kDa antibody
    • Signal recognition particle subunit SRP72 antibody
    • SRP72 antibody
    • SRP72_HUMAN antibody
    see all

Images

  • All lanes : Anti-SRP72 antibody [EPR17143] (ab200199) at 1/5000 dilution

    Lane 1 : K562 (Human chronic myelogenous leukemia cells from bone marrow) cell lysate
    Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 75 kDa
    Observed band size: 75 kDa


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-SRP72 antibody [EPR17143] (ab200199) at 1/5000 dilution + Jurkat (Human T cell leukemia cells from peripheral blood) cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 75 kDa
    Observed band size: 75 kDa


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-SRP72 antibody [EPR17143] (ab200199) at 1/1000 dilution + Human fetal brain lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 75 kDa
    Observed band size: 75 kDa


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling SRP72 with ab200199 at 1/100 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

References

ab200199 has not yet been referenced specifically in any publications.

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