Overview

  • Product name

    Anti-SRPK1 antibody [EPR6915(2)]
    See all SRPK1 primary antibodies
  • Description

    Rabbit monoclonal [EPR6915(2)] to SRPK1
  • Host species

    Rabbit
  • Specificity

    The immunogen used for this product shares 69% homology with SRPK2 and 67% homology with SRPK3. Cross-reactivity with this protein has not been confirmed experimentally.
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human SRPK1 (Q96SB4).

  • Positive control

    • Jurkat, BxPC-3 and Caco-2 cell lysates.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 1.91 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR6915(2)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab131160 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 92 kDa (predicted molecular weight: 74 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      Plays a central role in the regulatory network for splicing, controlling the intranuclear distribution of splicing factors in interphase cells and the reorganization of nuclear speckles during mitosis. Hyperphosphorylates RS domain-containing proteins such as SFRS1, SFRS2 and ZRSR2 on serine residues during metaphase but at lower levels during interphase. Locks onto SFRS1 to form a stable complex and processively phosphorylates the RS domain. Appears to mediate HBV core protein phosphorylation which is a prerequisite for pregenomic RNA encapsidation into viral capsids.
    • Tissue specificity

      Isoform 2 is predominantly expressed in the testis but is also present at lower levels in heart, ovary, small intestine, liver, kidney, pancreas and skeletal muscle. Isoform 1 is only seen in the testis, at lower levels than isoform 2.
    • Sequence similarities

      Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family.
      Contains 1 protein kinase domain.
    • Cellular localization

      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • Serine/arginine rich protein specific kinase 1 antibody
      • Serine/arginine rich splicing factor kinase 1 antibody
      • Serine/arginine-rich protein-specific kinase 1 antibody
      • Serine/threonine protein kinase SRPK1 antibody
      • Serine/threonine-protein kinase SRPK1 antibody
      • SFRS protein kinase 1 antibody
      • SFRSK1 antibody
      • SR protein kinase 1 antibody
      • SR protein specific kinase 1 antibody
      • SR-protein-specific kinase 1 antibody
      • SRPK1 antibody
      • SRPK1_HUMAN antibody
      see all

    Images

    • All lanes : Anti-SRPK1 antibody [EPR6915(2)] (ab131160) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : BxPC-3 cell lysate
      Lane 3 : Caco-2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 74 kDa
      Observed band size: 92 kDa
      why is the actual band size different from the predicted?

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab131160 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab131160.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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