Product nameAnti-SRPK1 antibody [EPR6915(2)]
See all SRPK1 primary antibodies
DescriptionRabbit monoclonal [EPR6915(2)] to SRPK1
SpecificityThe immunogen used for this product shares 69% homology with SRPK2 and 67% homology with SRPK3. Cross-reactivity with this protein has not been confirmed experimentally.
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP
Species reactivityReacts with: Mouse, Human
Synthetic peptide, corresponding to residues in Human SRPK1 (Q96SB4).
- Jurkat, BxPC-3 and Caco-2 cell lysates.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.91 x 10 -10 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab131160 in the following tested applications.
|WB||1/1000 - 1/10000. Detects a band of approximately 92 kDa (predicted molecular weight: 74 kDa).|
FunctionPlays a central role in the regulatory network for splicing, controlling the intranuclear distribution of splicing factors in interphase cells and the reorganization of nuclear speckles during mitosis. Hyperphosphorylates RS domain-containing proteins such as SFRS1, SFRS2 and ZRSR2 on serine residues during metaphase but at lower levels during interphase. Locks onto SFRS1 to form a stable complex and processively phosphorylates the RS domain. Appears to mediate HBV core protein phosphorylation which is a prerequisite for pregenomic RNA encapsidation into viral capsids.
Tissue specificityIsoform 2 is predominantly expressed in the testis but is also present at lower levels in heart, ovary, small intestine, liver, kidney, pancreas and skeletal muscle. Isoform 1 is only seen in the testis, at lower levels than isoform 2.
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family.
Contains 1 protein kinase domain.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- Serine/arginine rich protein specific kinase 1 antibody
- Serine/arginine rich splicing factor kinase 1 antibody
- Serine/arginine-rich protein-specific kinase 1 antibody
All lanes : Anti-SRPK1 antibody [EPR6915(2)] (ab131160) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : BxPC-3 cell lysate
Lane 3 : Caco-2 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 74 kDa
Observed band size: 92 kDa why is the actual band size different from the predicted?
Equilibrium disassociation constant (KD)
Learn more about KD
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ab131160 has not yet been referenced specifically in any publications.