Key features and details
- Rabbit polyclonal to SRPX2
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-SRPX2 antibody
See all SRPX2 primary antibodies
DescriptionRabbit polyclonal to SRPX2
Specificityab91584 is specific for SRPX2 and will not recognize SRPX1
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
A 16 amino acid synthetic peptide from near the amino terminus of Human SRPX2 (NP_055282).
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab91584 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 20 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 53 kDa.|
|IHC-P||Use at an assay dependent concentration.|
FunctionActs as a ligand for the urokinase plasminogen activator surface receptor. Plays a role in angiogenesis by inducing endothelial cell migration and the formation of vascular network (cords). Involved in cellular migration and adhesion in cancer cells. Increases the phosphorylation levels of FAK. May have a role in the perisylvian region, critical for language and cognitive development.
Tissue specificityExpressed in neurons of the rolandic area of the brain (at protein level). Highly expressed in the brain, placenta, lung, trachea, uterus and adrenal gland. Weakly expressed in the peripheral blood, brain and bone marrow. Expressed in numerous cancer cell lines.
Involvement in diseaseDefects in SRPX2 are a cause of bilateral perisylvian polymicrogyria (BPP) [MIM:300388]. BPP is the most common form of polymicrogyria, a malformation of the cortex, in which the brain surface is irregular and the normal gyral pattern replaced by multiple small, partly fused, gyri separated by shallow sulci. BPP results in mild mental retardation, epilepsy and pseudobulbar palsy, causing difficulties with expressive speech and feeding.
Defects in SRPX2 are a cause of rolandic epilepsy with speech dyspraxia and mental retardation X-linked (RESDX) [MIM:300643]. A condition characterized by the association of rolandic seizures with oral and speech dyspraxia, and mental retardation. Rolandic occur during a period of significant brain maturation. During this time, dysfunction of neural network activities such as focal discharges may be associated with specific developmental disabilities resulting in specific cognitive impairments of language, visuo-spatial abilities or attention.
Sequence similaritiesContains 1 HYR domain.
Contains 3 Sushi (CCP/SCR) domains.
Cellular localizationCytoplasm. Secreted.
- Information by UniProt
- BPP antibody
- CBPS antibody
- PMGX antibody
Lane 1 : Anti-SRPX2 antibody (ab91584) at 1 µg/ml
Lane 2 : Anti-SRPX2 antibody (ab91584) at 2 µg/ml
All lanes : Human lung tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 53 kDa
Immunofluorescence of SRPX2 in Human lung tissue with ab91584 at 20 ug/mL.
Immunohistochemistry of rat lung tissue with ab91584 at 5 μg/mL.
ab91584 has been referenced in 3 publications.
- Zhang M et al. High SRPX2 protein expression predicts unfavorable clinical outcome in patients with prostate cancer. Onco Targets Ther 11:3149-3157 (2018). PubMed: 29881288
- Anwer M et al. Sushi repeat-containing protein X-linked 2: A novel phylogenetically conserved hypothalamo-pituitary protein. J Comp Neurol 526:1806-1819 (2018). PubMed: 29663392
- Gäbel G et al. Molecular Fingerprint for Terminal Abdominal Aortic Aneurysm Disease. J Am Heart Assoc 6:N/A (2017). PubMed: 29191809