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    srsf3-antibody-ab125124.pdf

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Epigenetics and Nuclear Signaling DNA / RNA RNA Processing Splicing
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Anti-SRSF3 antibody (ab125124)

  • Datasheet
Reviews (3)Q&A (1)References (4)

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Western blot - Anti-SRSF3 antibody (ab125124)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRSF3 antibody (ab125124)
  • Western blot - Anti-SRSF3 antibody (ab125124)
  • Immunocytochemistry/ Immunofluorescence - Anti-SRSF3 antibody (ab125124)

Key features and details

  • Rabbit polyclonal to SRSF3
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-SRSF3 antibody
    See all SRSF3 primary antibodies
  • Description

    Rabbit polyclonal to SRSF3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human SRSF3 (C terminal).

  • General notes

     This product was previously labelled as SFRS3

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride

    PBS (without Mg2+, Ca2+)
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Splicing

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa nuclear extract lysate (ab14655)
  • Recombinant Protein

    • Recombinant Human SRSF3 protein (ab114675)

Applications

Our Abpromise guarantee covers the use of ab125124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 19 kDa.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF 1/100 - 1/500.

Target

  • Function

    May be involved in RNA processing in relation with cellular proliferation and/or maturation.
  • Sequence similarities

    Belongs to the splicing factor SR family.
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications

    Extensively phosphorylated on serine residues in the RS domain.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P84103 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6428 Human
    • Omim: 603364 Human
    • SwissProt: P84103 Human
    • Unigene: 405144 Human
    • Alternative names

      • arginine/serine-rich 3 antibody
      • Pre mRNA splicing factor SRP20 antibody
      • Pre-mRNA-splicing factor SRP20 antibody
      • Serine/arginine-rich splicing factor 3 antibody
      • Splicing factor antibody
      • Splicing factor arginine/serine rich 20 kD antibody
      • Splicing factor arginine/serine rich 3 antibody
      • SRp20 antibody
      • SRSF3 antibody
      • SRSF3_HUMAN antibody
      • X16 antibody
      see all

    Images

    • Western blot - Anti-SRSF3 antibody (ab125124)
      Western blot - Anti-SRSF3 antibody (ab125124)This image is courtesy of Suypriya Sen

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRSF3 antibody (ab125124)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRSF3 antibody (ab125124)

      ab125124, at 5ug/ml, staining SRSF3 in formalin-fixed, paraffin-embedded Human testis tissue by Immunohistochemistry.

    • Western blot - Anti-SRSF3 antibody (ab125124)
      Western blot - Anti-SRSF3 antibody (ab125124)
      All lanes : Anti-SRSF3 antibody (ab125124) at 1/500 dilution

      Lane 1 : HeLa cell extract
      Lane 2 : Jurkat cell extract
      Lane 3 : Jurkat cell extract with synthesized peptide


      Predicted band size: 19 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-SRSF3 antibody (ab125124)
      Immunocytochemistry/ Immunofluorescence - Anti-SRSF3 antibody (ab125124)

      ab125124 at 1/100 dilution staining SRSF3 in MCF7 cells by Immunofluorescence. The image on the right is treated with the synthesized peptide.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (4)

    Publishing research using ab125124? Please let us know so that we can cite the reference in this datasheet.

    ab125124 has been referenced in 4 publications.

    • Simsek D  et al. The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity. Cell 169:1051-1065.e18 (2017). PubMed: 28575669
    • Lin YJ  et al. P-coumaric acid regulates exon 12 splicing of the ATP7B gene by modulating hnRNP A1 protein expressions. Biomedicine (Taipei) 5:10 (2015). PubMed: 26048696
    • Ajiro M  et al. Adapted Resistance to the Knockdown Effect of shRNA-Derived Srsf3 siRNAs in Mouse Littermates. Int J Biol Sci 11:1248-56 (2015). PubMed: 26435690
    • Sen S  et al. Deletion of splicing factor SRSF3 in hepatocytes predisposes to hepatocellular carcinoma in mice. Hepatology N/A:N/A (2014). Mouse . PubMed: 25132062

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SRSF3 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate
    Permeabilization
    No
    Specification
    Liver
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Dr. Suypriya Sen

    Verified customer

    Submitted Sep 05 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SRSF3 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate
    Permeabilization
    No
    Specification
    liver
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Dr. Suypriya Sen

    Verified customer

    Submitted Aug 25 2014

    Western blot abreview for Anti-SRSF3 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Primary Hepatocytes)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    25 µg
    Specification
    Primary Hepatocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Dr. Suypriya Sen

    Verified customer

    Submitted May 08 2013

    Question

    Dear technical support,

    I am looking to purchase an antibody against SFRS3 in mouse cells/heart section. I'm trying to choose between ab125124 and ab73891, both of which are predicted to react with mouse. I am confused since 2 of the submitted images attached to reviews are identical between both product codes. Please could you tell me if there is in fact a difference between these products and to which product the images relate?

    Many thanks in advance,

    Read More

    Abcam community

    Verified customer

    Asked on Jul 17 2012

    Answer

    Thank you for your enquiry.

    I can confirm you are correct, these are the same antibody. We have sourced the same antibody from two different places and given them different product codes. The sources have tested them in different applications, which is why we have chosen to keep both on the catalog, to provide a wider selection of suitably tested antibodies for customers.

    I hope this helps to clarify any confusion. If you have any further questions, please do not hesitate to contact us.

    Read More

    Abcam Scientific Support

    Answered on Jul 17 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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