Anti-SRSF3 antibody (ab125124)

Reviews (3)Q&A (1)References (4)

Overview

  • Product name

    Anti-SRSF3 antibody
    See all SRSF3 primary antibodies

  • Description

    Rabbit polyclonal to SRSF3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, ELISAmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide corresponding to Human SRSF3 (C terminal).

  • Positive control

    • MCF7 cells; HeLa and Jurkat cell extracts; Human Testis tissue.

  • General notes

     This product was previously labelled as SFRS3

     

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
    Note: PBS (without Mg2+, Ca2+)
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab125124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 19 kDa.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF 1/100 - 1/500.
ELISA 1/5000.

Target

  • Function

    May be involved in RNA processing in relation with cellular proliferation and/or maturation.

  • Sequence similarities

    Belongs to the splicing factor SR family.
    Contains 1 RRM (RNA recognition motif) domain.

  • Post-translational
    modifications

    Extensively phosphorylated on serine residues in the RS domain.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • arginine/serine-rich 3 antibody
      • Pre mRNA splicing factor SRP20 antibody
      • Pre-mRNA-splicing factor SRP20 antibody
      • Serine/arginine-rich splicing factor 3 antibody
      • Splicing factor antibody
      • Splicing factor arginine/serine rich 20 kD antibody
      • Splicing factor arginine/serine rich 3 antibody
      • SRp20 antibody
      • SRSF3 antibody
      • SRSF3_HUMAN antibody
      • X16 antibody
      see all

    Images

    • Western blot - Anti-SRSF3 antibody (ab125124)
      Western blot - Anti-SRSF3 antibody (ab125124)This image is courtesy of Suypriya Sen

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRSF3 antibody (ab125124)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRSF3 antibody (ab125124)

      ab125124, at 5ug/ml, staining SRSF3 in formalin-fixed, paraffin-embedded Human testis tissue by Immunohistochemistry.

    • Western blot - Anti-SRSF3 antibody (ab125124)
      Western blot - Anti-SRSF3 antibody (ab125124)
      All lanes : Anti-SRSF3 antibody (ab125124) at 1/500 dilution

      Lane 1 : HeLa cell extract
      Lane 2 : Jurkat cell extract
      Lane 3 : Jurkat cell extract with synthesized peptide


      Predicted band size: 19 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-SRSF3 antibody (ab125124)
      Immunocytochemistry/ Immunofluorescence - Anti-SRSF3 antibody (ab125124)

      ab125124 at 1/100 dilution staining SRSF3 in MCF7 cells by Immunofluorescence. The image on the right is treated with the synthesized peptide.

    References

    This product has been referenced in:

    • Simsek D  et al. The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity. Cell 169:1051-1065.e18 (2017). Read more (PubMed: 28575669) »
    • Lin YJ  et al. P-coumaric acid regulates exon 12 splicing of the ATP7B gene by modulating hnRNP A1 protein expressions. Biomedicine (Taipei) 5:10 (2015). Read more (PubMed: 26048696) »
    See all 4 Publications for this product

    Publishing research using ab125124? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-4 of 4 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SRSF3 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate
    Permeabilization
    No
    Specification
    Liver
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Dr. Suypriya Sen

    Verified customer

    Submitted Sep 05 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SRSF3 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate
    Permeabilization
    No
    Specification
    liver
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Dr. Suypriya Sen

    Verified customer

    Submitted Aug 25 2014

    Western blot abreview for Anti-SRSF3 antibody

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Primary Hepatocytes)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    25 µg
    Specification
    Primary Hepatocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Dr. Suypriya Sen

    Verified customer

    Submitted May 08 2013

    Question

    Dear technical support,

    I am looking to purchase an antibody against SFRS3 in mouse cells/heart section. I'm trying to choose between ab125124 and ab73891, both of which are predicted to react with mouse. I am confused since 2 of the submitted images attached to reviews are identical between both product codes. Please could you tell me if there is in fact a difference between these products and to which product the images relate?

    Many thanks in advance,

    Read More
    Answer

    Thank you for your enquiry.

    I can confirm you are correct, these are the same antibody. We have sourced the same antibody from two different places and given them different product codes. The sources have tested them in different applications, which is why we have chosen to keep both on the catalog, to provide a wider selection of suitably tested antibodies for customers.

    I hope this helps to clarify any confusion. If you have any further questions, please do not hesitate to contact us.

    Read More

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