Overview

  • Product name

    Anti-SRSF3 antibody [EPR16976]
    See all SRSF3 primary antibodies
  • Description

    Rabbit monoclonal [EPR16976] to SRSF3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SRSF3 aa 50-150. The exact sequence is proprietary.
    Database link: P84103

  • Positive control

    • WB: Jurkat, HepG2, C6, RAW 264.7, PC-12, NIH/3T3 and K562 whole cell lysates. Mouse and Rat brain lysates. IHC-P: Human cervix carcinoma and Human cerebral cortex tissue. Mouse liver tissue. ICC/IF: HeLa, and PC-12 cells. FC: Jurkat cells.
  • General notes

     

     

     This product was previously labelled as SFRS3

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR16976
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab198291 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
IHC-P 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/500.
Flow Cyt 1/250.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    May be involved in RNA processing in relation with cellular proliferation and/or maturation.
  • Sequence similarities

    Belongs to the splicing factor SR family.
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications

    Extensively phosphorylated on serine residues in the RS domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • arginine/serine-rich 3 antibody
    • Pre mRNA splicing factor SRP20 antibody
    • Pre-mRNA-splicing factor SRP20 antibody
    • Serine/arginine-rich splicing factor 3 antibody
    • Splicing factor antibody
    • Splicing factor arginine/serine rich 20 kD antibody
    • Splicing factor arginine/serine rich 3 antibody
    • SRp20 antibody
    • SRSF3 antibody
    • SRSF3_HUMAN antibody
    • X16 antibody
    see all

Images

  • All lanes : Anti-SRSF3 antibody [EPR16976] (ab198291) at 1/5000 dilution

    Lane 1 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
    Lane 3 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 19 kDa
    Observed band size: 19 kDa



    Blocking/dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/Immunofluorescence analysis of PC-12 (Rat adrenal gland pheochromocytoma cell line) labelling SRSF3 with purified ab198291 at 1/500. Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

  • All lanes : Anti-SRSF3 antibody [EPR16976] (ab198291) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate
    Lane 3 : C6 (Rat glial tumor) whole cell lysate
    Lane 4 : RAW 264.7(Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 6 : NIH/3T3 (Mouse embryo fibroblast cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 19 kDa
    Observed band size: 19 kDa



    Blocking/dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling SRSF3 with ab198291 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on Human cervix carcinoma tissue is observed. Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary ab.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling SRSF3 with ab198291 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and weakly cytoplasm staining on Human cerebral cortex tissue is observed. Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary ab.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling SRSF3 with ab198291 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear and weakly cytoplasm staining on mouse liver tissue is observed. Counter stained with Hematoxylin.
    Negative control: Used PBS instead of primary ab.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T Cell Leukemia cells from peripheral blood) labeling SRSF3 with ab198291 at 1/440 dilution (red) compared with a Rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

References

This product has been referenced in:

  • Shen T  et al. Alternative polyadenylation dependent function of splicing factor SRSF3 contributes to cellular senescence. Aging (Albany NY) 11:1356-1388 (2019). Read more (PubMed: 30835716) »
  • Shen T  et al. Antisense transcription regulates the expression of sense gene via alternative polyadenylation. Protein Cell 9:540-552 (2018). Read more (PubMed: 29273853) »
See all 2 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK-293)
Permeabilization
Yes - 0.3% Triton X-100 in Blocking Buffer
Specification
HEK-293
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Mar 22 2018

Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (primary hepatoctyes)
Total protein in input
30 µg
Immuno-precipitation step
Protein A
Specification
primary hepatoctyes

Abcam user community

Verified customer

Submitted Dec 12 2016

Application
Western blot
Sample
Mouse Cell lysate - whole cell (primary hepatoctyes)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
25 µg
Specification
primary hepatoctyes
Blocking step
I-Block(Applied biosystems) as blocking agent for 30 mins as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2µg/mL · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 23 2016

Application
Western blot
Sample
Human Cell lysate - whole cell (Human Lymphocytes)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
40 µg
Specification
Human Lymphocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 02 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up