Anti-SRSF5 antibody (ab67175)
Key features and details
- Mouse polyclonal to SRSF5
- Suitable for: WB, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-SRSF5 antibody
See all SRSF5 primary antibodies -
Description
Mouse polyclonal to SRSF5 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human SRSF5. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
-
Positive control
- HEK-293T, HeLa and Hep G2 cell lysates; Human liver tissue lysate. Human ovary tissue sections. Hela cells. SRSF5 transfected 293T cell lysate
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
KO cell lines
-
KO cell lysates
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab67175 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 31 kDa).
|
IHC-P |
Use a concentration of 1.5 µg/ml.
|
|
ICC/IF |
Use at an assay dependent dilution. NOTE: Product requires purification with Protein A prior to use in ICC/IF and IHC-P.
|
Notes |
---|
WB
1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 31 kDa). |
IHC-P
Use a concentration of 1.5 µg/ml. |
ICC/IF
Use at an assay dependent dilution. NOTE: Product requires purification with Protein A prior to use in ICC/IF and IHC-P. |
Target
-
Function
Plays a role in constitutive splicing and can modulate the selection of alternative splice sites. -
Sequence similarities
Belongs to the splicing factor SR family.
Contains 2 RRM (RNA recognition motif) domains. -
Post-translational
modificationsExtensively phosphorylated on serine residues in the RS domain. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 6430 Human
- Omim: 600914 Human
- SwissProt: Q13243 Human
- Unigene: 632326 Human
-
Alternative names
- arginine/serine-rich 5 antibody
- Delayed early protein HRS antibody
- Delayed-early protein HRS antibody
see all
Images
-
All lanes : Anti-SRSF5 antibody (ab67175) at 1/500 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : SRSF5 knockout HEK-293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 31 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab67175 observed at 40 kDa. Red - loading control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55 kDa.
ab67175 was shown to react with SRSF5 in wild-type HEK-293T cells in Western blot with loss of signal observed in SRSF5 knockout cell line ab266246 (SRSF5 knockout cell lysate ab258704). Wild-type HEK-293T and SRSF5 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab67175 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4 °C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
-
ab67175, at 1.5µg/ml, staining SRSF5 in human ovary by immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
-
ICC/IF image of ab67175 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab67175, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
-
Anti-SRSF5 antibody (ab67175) at 1/500 dilution + human liver tissue lysate at 25 µg/ml
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 31 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted? -
All lanes : Anti-SRSF5 antibody (ab67175) at 1/500 dilution
Lane 1 : SRSF5 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg/ml per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 31 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?
Additional bands at: 27 kDa. We are unsure as to the identity of these extra bands.
Protocols
Datasheets and documents
-
Datasheet download
References (5)
ab67175 has been referenced in 5 publications.
- Zhang M et al. CPEB2 m6A methylation regulates blood-tumor barrier permeability by regulating splicing factor SRSF5 stability. Commun Biol 5:908 (2022). PubMed: 36064747
- Chen S et al. CLK1/SRSF5 pathway induces aberrant exon skipping of METTL14 and Cyclin L2 and promotes growth and metastasis of pancreatic cancer. J Hematol Oncol 14:60 (2021). PubMed: 33849617
- Li J et al. SRSF5 regulates alternative splicing of DMTF1 pre-mRNA through modulating SF1 binding. RNA Biol 18:318-336 (2021). PubMed: 34291726
- Wu P et al. Tumor Cell-Derived TGFß1 Attenuates Antitumor Immune Activity of T Cells via Regulation of PD-1 mRNA. Cancer Immunol Res 8:1470-1484 (2020). PubMed: 32999004
- Chen Y et al. Mutually exclusive acetylation and ubiquitylation of the splicing factor SRSF5 control tumor growth. Nat Commun 9:2464 (2018). PubMed: 29942010