Key features and details
- Mouse monoclonal [OTI3C8] to SRY/TDF
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-SRY/TDF antibody [OTI3C8]
See all SRY/TDF primary antibodies
DescriptionMouse monoclonal [OTI3C8] to SRY/TDF
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Macaque monkey, Gorilla, Orangutan
Recombinant full length protein corresponding to Human SRY/TDF aa 1-204.
MQSYASAMLSVFNSDDYSPAVQENIPALRRSSSFLCTESCNSKYQCETGE NSKGNVQDRVKRPMNAFIVW SRDQRRKMALENPRMRNSEISKQLGYQW KMLTEAEKWPFFQEAQKLQAMHREKYPNYKYRPRRKAKMLPK NCSLLP ADPASVLCSEVQLDNRLYRDDCTKATHSRMEHQLGHLPPINAASSPQQRD RYSHWTKL
Database link: NP_003131
- Purchase matching WB positive control:Recombinant Human SRY/TDF protein
- WB: HEK293T cells transfected with pCMV6-ENTRY SRY/TDF cDNA; ICC/IF: COS7 cells transiently transfected by pCMV6-ENTRY SRY/TDF.
The clone number has been updated from 3C8 to OTI3C8, both clone numbers name the same clone.
This product was previously labelled as SRY
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 1% BSA, PBS
Concentration information loading...
Purification notesPurified from cell culture supernatant by affinity chromatography
Our Abpromise guarantee covers the use of ab140309 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 24 kDa.|
FunctionTranscriptional regulator that controls a genetic switch in male development. It is necessary and sufficient for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) as Sertoli rather than granulosa cells (By similarity). In male adult brain involved in the maintenance of motor functions of dopaminergic neurons (By similarity). Involved in different aspects of gene regulation including promoter activation or repression (By similarity). Promotes DNA bending. SRY HMG box recognizes DNA by partial intercalation in the minor groove. Also involved in pre-mRNA splicing. Binds to the DNA consensus sequence 5'-[AT]AACAA[AT]-3'.
Involvement in diseaseDefects in SRY are a cause of gonadal dysgenesis XY female type (GDXY) [MIM:400044]; also known as complete SRY-related 46,XY gonadal dysgenesis or 'XY females' or Swyer syndrome. Patients are found to have a 46,XY karyotype. They suffer rapid and early degeneration of their gonads, which are present in the adult as 'streak gonads', consisting mainly of fibrous tissue and variable amounts of ovarian stroma. As a result these patients do not develop secondary sexual characteristics at puberty. The external genitalia in these subjects are completely female, and Muellerian structures are normal. In contrast, subjects with 46,XY partial gonadal dysgenesis have ambiguous genitalia, a mix of Muellerian and Wolffian structures, and dysgenic gonads.
Note=A 45,X chromosomal aberration involving SRY is found in Turner syndrome, a disease characterized by gonadal dysgenesis with short stature, "streak gonads", variable abnormalities such as webbing of the neck, cubitus valgus, cardiac defects, low posterior hair line. The phenotype is female.
Defects in SRY are a cause of true hermaphroditism (TRUHER) [MIM:400045]; also known as complete SRY-positive 46,XX gonadal dysgenesis. A true hermaphrodite must have both mature ovarian and mature testicular tissue with histologic evidence of follicles and tubules, respectively. It is a genetically heterogeneous condition. The genotype of most affected individuals is 46,XX, but many have 46,XY or a mosaic of 46,XX/46,XY. True hermaphroditism can be caused also by chromosomal translocation.
Sequence similaritiesBelongs to the SRY family.
Contains 1 HMG box DNA-binding domain.
DomainDNA binding and bending properties of the HMG domains of human and mouse SRY differ form each other. Human SRY shows more extensive minor groove contacts with DNA and a lower specificity of sequence recognition than mouse SRY.
modificationsPhosphorylated on serine residues by PKA. Phosphorylation by PKA enhances its DNA-binding activity and stimulates transcription repression. Acetylation of Lys-136 contributes to its nuclear localization and enhances its interaction with KPNB1. Deacetylated by HDAC3. Poly-ADP-ribosylated by PARP1. ADP-ribosylation reduces its DNA-binding activity.
Cellular localizationNucleus speckle. Cytoplasm. Colocalizes with SOX6 in speckles. Colocalizes with CAML in the nucleus. Colocalizes in the nucleus with ZNF208 isoform KRAB-O and tyrosine hydroxylase (TH).
- Information by UniProt
- Essential protein for sex determination in human males antibody
- Sex determining region on Y antibody
- Sex determining region protein antibody
Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SRY/TDF, labelling SRY/TDF with ab140309 at 1/100 dilution.
All lanes : Anti-SRY/TDF antibody [OTI3C8] (ab140309) at 1/2000 dilution
Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA.
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY SRY/TDF cDNA.
Lysates/proteins at 5 µg per lane.
Predicted band size: 24 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab140309 has not yet been referenced specifically in any publications.