Key features and details
- Rabbit polyclonal to SS18
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SS18 antibody
See all SS18 primary antibodies
DescriptionRabbit polyclonal to SS18
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Recombinant fragment within Human SS18 (internal sequence). The exact sequence is proprietary.
Database link: Q15532
- WB: A549 , H1299 , HCT 116 cells. IP: HCT 116 cells.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272904 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000.
1/100 - 1/500.
1/500 - 1/3000.
1/100 - 1/500.
FunctionAppears to function synergistically with RBM14 as a transcriptional coactivator. Isoform 1 and isoform 2 function in nuclear receptor coactivation. Isoform 1 and isoform 2 function in general transcriptional coactivation.
Tissue specificityFairly ubiquitously expressed. Expressed in synovial sarcomas and in other human cell lines. The fusion genes SSXT-SSX1 and SSXT-SSX2 are expressed only in synovial sarcomas.
Involvement in diseaseNote=A chromosomal aberration involving SS18 may be a cause of synovial sarcoma. Translocation t(X;18)(p11.2;q11.2). The translocation is specifically found in more than 80% of synovial sarcoma. The fusion products SSXT-SSX1 or SSXT-SSX2 are probably responsible for transforming activity. Heterogeneity in the position of the breakpoint can occur (low frequency).
Sequence similaritiesBelongs to the SS18 family.
- Information by UniProt
- MGC116875 antibody
- Protein SSXT antibody
- Protein SYT antibody
All lanes : Anti-SS18 antibody (ab272904) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : SS18 knockout HeLa cell lysate
Lane 3 : HEK-293 cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Observed band size: 51/60 kDa why is the actual band size different from the predicted?
False colour image of Western blot: Anti-SS18 antibody staining at 1/500 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab272904 was shown to bind specifically to SS18. A band was observed at 51/60 kDa in wild-type HeLa cell lysates with no signal observed at this size in SS18 knockout cell line ab265493 (knockout cell lysate ab259156). To generate this image, wild-type and SS18 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes : Anti-SS18 antibody (ab272904) at 1/1000 dilution
Lane 1 : A549 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lane 3 : HCT 116 whole cell lysate
Lysates/proteins at 30 µg per lane.
Immunoprecipitation of SS18 protein from HCT116 whole cell extracts using 5 μg of ab272904.
Western blot analysis was performed using ab272904 with 1/500 dilution.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab272904 has not yet been referenced specifically in any publications.