Anti-SSB antibody (ab133171)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-SSB antibody
See all SSB primary antibodies -
Description
Rabbit polyclonal to SSB -
Host species
Rabbit -
Specificity
ab133171 detects endogenous levels of total SSB protein. -
Tested applications
Suitable for: WB, ELISA, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide from the C-terminus of Human SSB.
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Positive control
- COLO cell extract; Human breast tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
Note: PBS is without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab133171 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Predicted molecular weight: 46 kDa. | |
| ELISA | 1/40000. | |
| IHC-P | Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Binds to the 3' poly(U) terminii of nascent RNA polymerase III transcripts, protecting them from exonuclease digestion and facilitating their folding and maturation. -
Sequence similarities
Contains 1 HTH La-type RNA-binding domain.
Contains 1 RRM (RNA recognition motif) domain. -
Post-translational
modificationsPhosphorylated. The phosphorylation sites are at the C-terminal part of the protein.
The N-terminus is blocked. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6741 Human
- GenBank: NP_003133.1 Human
- Omim: 109090 Human
- SwissProt: P05455 Human
- Unigene: 632535 Human
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Alternative names
- Autoantigen La antibody
- La antibody
- La autoantigen antibody
see all
Images
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Western blot - Anti-SSB antibody (ab133171)All lanes : Anti-SSB antibody (ab133171) at 1/500 dilution
Lane 1 : COLO cell extract
Lane 2 : COLO cell extract with synthesized
peptide
Predicted band size: 46 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSB antibody (ab133171)Immunohistochemical analysis of paraffin embedded Human breast tissue labelling SSB with ab133171 antibody at a concentration of 10 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Datasheets and documents
References
ab133171 has not yet been referenced specifically in any publications.
