Anti-SSB antibody (ab226196)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-SSB antibody
See all SSB primary antibodies -
Description
Rabbit polyclonal to SSB -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human
Predicted to work with: Rabbit, Cow
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Immunogen
Synthetic peptide within Human SSB aa 358-408. The exact sequence is proprietary. (NP_003133.1).
Database link: P05455 -
Positive control
- IHC-P: Human ovarian carcinoma and breast carcinoma tissues. WB: HEK-293T, HeLa and Jurkat whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab226196 was affinity purified using an epitope specific to SSB immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab226196 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/2000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/2000 - 1/10000. Predicted molecular weight: 47 kDa. |
Target
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Function
Binds to the 3' poly(U) terminii of nascent RNA polymerase III transcripts, protecting them from exonuclease digestion and facilitating their folding and maturation. -
Sequence similarities
Contains 1 HTH La-type RNA-binding domain.
Contains 1 RRM (RNA recognition motif) domain. -
Post-translational
modificationsPhosphorylated. The phosphorylation sites are at the C-terminal part of the protein.
The N-terminus is blocked. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6741 Human
- Entrez Gene: 100008866 Rabbit
- GenBank: NP_003133.1 Human
- Omim: 109090 Human
- SwissProt: P10881 Cow
- SwissProt: P05455 Human
- Unigene: 632535 Human
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Alternative names
- Autoantigen La antibody
- La antibody
- La autoantigen antibody
see all
Images
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Western blot - Anti-SSB antibody (ab226196)All lanes : Anti-SSB antibody (ab226196) at 0.1 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 47 kDa
Exposure time: 10 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSB antibody (ab226196)Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for SSB using ab226196 at 1/5000 dilution in immunohistochemical analysis. Detection: DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSB antibody (ab226196)Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for SSB using ab226196 at 1/5000 dilution in immunohistochemical analysis. Detection: DAB staining.
Datasheets and documents
References
ab226196 has not yet been referenced specifically in any publications.
