Product nameAnti-SSB antibody
See all SSB primary antibodies
DescriptionRabbit polyclonal to SSB
Tested applicationsSuitable for: IHC-P, WBmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Cow
Synthetic peptide within Human SSB aa 358-408. The exact sequence is proprietary. (NP_003133.1).
Database link: P05455
- IHC-P: Human ovarian carcinoma and breast carcinoma tissues. WB: HEK-293T, HeLa and Jurkat whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226196 was affinity purified using an epitope specific to SSB immobilized on solid support.
Our Abpromise guarantee covers the use of ab226196 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/2000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 47 kDa.|
FunctionBinds to the 3' poly(U) terminii of nascent RNA polymerase III transcripts, protecting them from exonuclease digestion and facilitating their folding and maturation.
Sequence similaritiesContains 1 HTH La-type RNA-binding domain.
Contains 1 RRM (RNA recognition motif) domain.
modificationsPhosphorylated. The phosphorylation sites are at the C-terminal part of the protein.
The N-terminus is blocked.
- Information by UniProt
- Autoantigen La antibody
- La antibody
- La autoantigen antibody
All lanes : Anti-SSB antibody (ab226196) at 0.1 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 47 kDa
Exposure time: 10 seconds
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for SSB using ab226196 at 1/5000 dilution in immunohistochemical analysis. Detection: DAB staining.
Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for SSB using ab226196 at 1/5000 dilution in immunohistochemical analysis. Detection: DAB staining.
ab226196 has not yet been referenced specifically in any publications.