Anti-SSB antibody (ab226196)
Key features and details
- Rabbit polyclonal to SSB
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SSB antibody
See all SSB primary antibodies -
Description
Rabbit polyclonal to SSB -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rabbit, Cow
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Immunogen
Synthetic peptide within Human SSB aa 358-408. The exact sequence is proprietary. (NP_003133.1).
Database link: P05455 -
Positive control
- IHC-P: Human ovarian carcinoma and breast carcinoma tissues. WB: HEK-293T, HeLa and Jurkat whole cell lysates.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab226196 was affinity purified using an epitope specific to SSB immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab226196 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/2000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/2000 - 1/10000. Predicted molecular weight: 47 kDa. |
Target
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Function
Binds to the 3' poly(U) terminii of nascent RNA polymerase III transcripts, protecting them from exonuclease digestion and facilitating their folding and maturation. -
Sequence similarities
Contains 1 HTH La-type RNA-binding domain.
Contains 1 RRM (RNA recognition motif) domain. -
Post-translational
modificationsPhosphorylated. The phosphorylation sites are at the C-terminal part of the protein.
The N-terminus is blocked. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6741 Human
- Entrez Gene: 100008866 Rabbit
- GenBank: NP_003133.1 Human
- Omim: 109090 Human
- SwissProt: P10881 Cow
- SwissProt: P05455 Human
- Unigene: 632535 Human
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Alternative names
- Autoantigen La antibody
- La antibody
- La autoantigen antibody
see all
Images
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Western blot - Anti-SSB antibody (ab226196)All lanes : Anti-SSB antibody (ab226196) at 0.1 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 47 kDa
Exposure time: 10 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSB antibody (ab226196)Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for SSB using ab226196 at 1/5000 dilution in immunohistochemical analysis. Detection: DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSB antibody (ab226196)Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for SSB using ab226196 at 1/5000 dilution in immunohistochemical analysis. Detection: DAB staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab226196 has not yet been referenced specifically in any publications.
