Product nameAnti-SSB antibody [EPR6570]
See all SSB primary antibodies
DescriptionRabbit monoclonal [EPR6570] to SSB
Tested applicationsSuitable for: WB, IP, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human SSB aa 100-200. The exact sequence is proprietary.
- Jurkat, K562, HeLa, Ramos, C6, PC12, NIH 3T3 and RAW 264.7 cell lysates; Human colon tissue; Human thyroid adenocarcinoma tissue
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 4.80 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124932 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 47 kDa (predicted molecular weight: 46 kDa).|
|IP||1/10 - 1/100.|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/50 - 1/100.|
FunctionBinds to the 3' poly(U) terminii of nascent RNA polymerase III transcripts, protecting them from exonuclease digestion and facilitating their folding and maturation.
Sequence similaritiesContains 1 HTH La-type RNA-binding domain.
Contains 1 RRM (RNA recognition motif) domain.
modificationsPhosphorylated. The phosphorylation sites are at the C-terminal part of the protein.
The N-terminus is blocked.
- Information by UniProt
- Autoantigen La antibody
- La antibody
- La autoantigen antibody
All lanes : Anti-SSB antibody [EPR6570] (ab124932) at 1/10000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Ramos cell lysate
Lane 5 : C6 cell lysate
Lane 6 : PC12 cell lysate
Lane 7 : NIH 3T3 cell lysate
Lane 8 : RAW 264.7 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled Goat anti Rabbit at 1/2000 dilution
Predicted band size: 46 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted?
Formalin-fixed, paraffin-embedded human colon tissue stained for SSB with ab124932 (1/250 dilution) in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human thyroid adenocarcinoma tissue stained for SSB with ab124932 (1/250 dilution) in immunohistochemical analysis.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab124932 has not yet been referenced specifically in any publications.