
Recombinant Anti-SSBP2 antibody [EPR11520] (ab177944)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-SSBP2 antibody [EPR11520]
See all SSBP2 primary antibodies -
Description
Rabbit monoclonal [EPR11520] to SSBP2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SSBP2 aa 300 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P81877 -
Positive control
- Jurkat, K562, Raji and fetal heart lysates; Jurkat cells; Human colon tissue and Human squamous cell carcinoma tissue.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11520 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab177944 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/1000 - 1/5000. Predicted molecular weight: 38 kDa. | |
IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Tissue specificity
Ubiquitous. -
Sequence similarities
Contains 1 LisH domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 23635 Human
- Entrez Gene: 66970 Mouse
- Entrez Gene: 361877 Rat
- Omim: 607389 Human
- SwissProt: P81877 Human
- SwissProt: Q9CYZ8 Mouse
- SwissProt: Q9R050 Rat
- Unigene: 102735 Human
see all -
Alternative names
- HSPC116 antibody
- Sequence specific single stranded DNA binding protein 2 antibody
- Sequence-specific single-stranded-DNA-binding protein 2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSBP2 antibody [EPR11520] (ab177944)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SSBP2 with ab177944 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-SSBP2 antibody [EPR11520] (ab177944) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : K562 cell lysate
Lane 3 : Raji cell lysate
Lane 4 : Fetal heart cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 38 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSBP2 antibody [EPR11520] (ab177944)
Immunohistochemical analysis of paraffin-embedded Human squamous cell carcinoma tissue labeling SSBP2 with ab177944 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Flow cytometric analysis of permeabilized Jurkat cells labeling SSBP2 with ab177944 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).
Datasheets and documents
References
This product has been referenced in:
- Kim H et al. Single-stranded DNA binding protein 2 expression is associated with patient survival in hepatocellular carcinoma. BMC Cancer 18:1244 (2018). Read more (PubMed: 30541499) »