Recombinant
RabMAb

Recombinant Anti-SSBP2 antibody [EPR11520] (ab177944)

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Overview

  • Product name

    Anti-SSBP2 antibody [EPR11520]
    See all SSBP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR11520] to SSBP2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SSBP2 aa 300 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P81877

  • Positive control

    • Jurkat, K562, Raji and fetal heart lysates; Jurkat cells; Human colon tissue and Human squamous cell carcinoma tissue.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR11520

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab177944 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 38 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC/IF or IP.

    • Target

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSBP2 antibody [EPR11520] (ab177944)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSBP2 antibody [EPR11520] (ab177944)

      Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SSBP2 with ab177944 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-SSBP2 antibody [EPR11520] (ab177944)
      Western blot - Anti-SSBP2 antibody [EPR11520] (ab177944)
      All lanes : Anti-SSBP2 antibody [EPR11520] (ab177944) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : Raji cell lysate
      Lane 4 : Fetal heart cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 38 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSBP2 antibody [EPR11520] (ab177944)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSBP2 antibody [EPR11520] (ab177944)

      Immunohistochemical analysis of paraffin-embedded Human squamous cell carcinoma tissue labeling SSBP2 with ab177944 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Flow Cytometry - Anti-SSBP2 antibody [EPR11520] (ab177944)
      Flow Cytometry - Anti-SSBP2 antibody [EPR11520] (ab177944)

      Flow cytometric analysis of permeabilized Jurkat cells labeling SSBP2 with ab177944 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

    References

    This product has been referenced in:

    • Kim H  et al. Single-stranded DNA binding protein 2 expression is associated with patient survival in hepatocellular carcinoma. BMC Cancer 18:1244 (2018). Read more (PubMed: 30541499) »
    See 1 Publication for this product

    Publishing research using ab177944? Please let us know so that we can cite the reference in this datasheet.

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