Overview

  • Product name
    Anti-SSEA3 antibody [MC631]
  • Description
    Rat monoclonal [MC631] to SSEA3
  • Host species
    Rat
  • Tested applications
    Suitable for: IHC-FoFr, WB, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    4-8 cell stage mouse embryos.

  • Positive control
    • 2102Ep Human Embryonic Carcinoma Cells
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Purity
    Tissue culture supernatant
  • Purification notes
    Tissue culture supernatant was cross flow concentrated and buffer exchanged to PBS
  • Clonality
    Monoclonal
  • Clone number
    MC631
  • Myeloma
    P3x63-Ag8.653
  • Isotype
    IgM
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab16286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
Flow Cyt 1/10 - 1/20.

 

ab35768 - Rat monoclonal IgM, is suitable for use as an isotype control with this antibody.

 

ICC/IF Use at an assay dependent concentration.

Target

  • Relevance
    SSEA3 is a globoseries carbohydrate antigen present on both cell surface glycolipids and glycopeptides. It is found on the surface of mouse oocytes, becoming restricted to the intracellular mass of the early blastocyst and then to the primitive endoderm. It is present on the surface of human teratocarcinoma cells (EC), embryonic germ cells (EG) and embryonic stem cells (ES) but not mouse undifferentiated mouse cells of these types. SSEA3 expression decreases as human EC, ES and EG cells differentiate but increases on differentiation of mouse EC, ES and EG cells. It is also found on the surface of mouse erythrocytes.
  • Alternative names
    • Stage specific embryonic antigen 3 antibody

Images

  • Human induced pluripotent stem cells (iPSCs) stained with ab16286 (red fill). In brief, iPSCs were fixed in 4% formaldehyde (methanol-free) for 15 min at 25°C. Cells were then incubated with the antibody (ab16286, 1/50 dilution) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 647 goat anti-rat IgG at 1/2000 dilution for 30 min at 4°C. iPSCs differentiated for 28 days toward a cardiomyocyte lineage (blue fill) were used as a negative control. Acquisition of >30,000 total events were collected using a 100mW solid state diode laser (640nm) and 670/30 bandpass filter.

  • The image shows cell membrane staining of 2102Ep human embryonic carcinoma cells using SSEA3 specific antibody ab16286.
  • Anti-SSEA3 antibody, ab16286 can be used as a marker of Embryonic Carcinoma cells in Flow Cytometry/FACS. The top histogram (A) shows undifferentiated NTERA2 cells (i.e. without retinoic acid) recognised by ab16286. However, upon differentiation (by the addition of retinoic acid 10-5M for 7 days), the antibody lost the ability to recognise these cells as shown in the bottom histogram (B).

References

This product has been referenced in:
  • Trevisan M  et al. Generation of a transgene-free induced pluripotent stem cells line (UNIPDi002-A) from oral mucosa epithelial stem cells carrying the R304Q mutation in TP63 gene. Stem Cell Res 28:149-152 (2018). Read more (PubMed: 29486400) »
  • Trevisan M  et al. Induced pluripotent stem cells line (UNIPDi003-A) from a patient affected by EEC syndrome carrying the R279H mutation in TP63 gene. Stem Cell Res 28:141-144 (2018). Human . Read more (PubMed: 29477592) »
See all 29 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human ES cell (hues7))
Specification
human ES cell (hues7)
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
1% serum 0.1% BSA in PBS as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C

Abcam user community

Verified customer

Submitted Jun 08 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (human ES cell undifferentiated)
Specification
human ES cell undifferentiated
Preparation
Cell harvesting/tissue preparation method: cell dissociation buffer
Sample buffer: FACS buffer (0.2% BSA, 0.1% sodium azide in PBS)
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
FSC vs SSC on negative control sample

Abcam user community

Verified customer

Submitted Jun 08 2012

Answer

Thanks for your reply. I would be happy to send a vial of ab32118. Could you just confirm your shipping address? Thank you, and I look forward to hearing from you.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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