Anti-SSEA3 antibody [MC631] (ab16286)

Reviews (2)Q&A (1)References (31)
Flow Cytometry - Anti-SSEA3 antibody [MC631] (ab16286)
  • Immunocytochemistry/ Immunofluorescence - Anti-SSEA3 antibody [MC631] (ab16286)
  • Flow Cytometry - Anti-SSEA3 antibody [MC631] (ab16286)

Key features and details

  • Rat monoclonal [MC631] to SSEA3
  • Suitable for: IHC-FoFr, Flow Cyt, ICC/IF
  • Reacts with: Human
  • Isotype: IgM

Overview

  • Product name

    Anti-SSEA3 antibody [MC631]

  • Description

    Rat monoclonal [MC631] to SSEA3

  • Host species

    Rat

  • Tested applications

    Suitable for: IHC-FoFr, Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse

  • Immunogen

    Tissue, cells or virus corresponding to Mouse SSEA3.

  • Positive control

    • 2102Ep Human Embryonic Carcinoma Cells

  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Purification notes

    Tissue culture supernatant was cross flow concentrated.

  • Clonality

    Monoclonal

  • Clone number

    MC631

  • Myeloma

    P3x63-Ag8.653

  • Isotype

    IgM

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab16286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr
Use at an assay dependent concentration.
Flow Cyt (1)
1/10 - 1/20.

 

ab35768 - Rat monoclonal IgM, is suitable for use as an isotype control with this antibody.

 
ICC/IF (1)
Use at an assay dependent concentration.
Notes
IHC-FoFr
Use at an assay dependent concentration.
Flow Cyt
1/10 - 1/20.

 

ab35768 - Rat monoclonal IgM, is suitable for use as an isotype control with this antibody.

 
ICC/IF
Use at an assay dependent concentration.

Target

  • Relevance

    SSEA3 is a globoseries carbohydrate antigen present on both cell surface glycolipids and glycopeptides. It was described and named as part of a series of embryonic antigens, defined by monoclonal antibodies isolated in the lab of Prof. Davor Solter (Shevinsky L. H. et al., 1982, Cell 30:697 and Kannagi R. et al., 1983, J. Biol. Chem. 258:8934). SSEA3 is found on the surface of mouse oocytes, becoming restricted to the intracellular mass of the early blastocyst and then to the primitive endoderm. It is present on the surface of human teratocarcinoma cells (EC), embryonic germ cells (EG) and embryonic stem cells (ES) but not mouse undifferentiated mouse cells of these types. SSEA3 expression decreases as human EC, ES and EG cells differentiate but increases on differentiation of mouse EC, ES and EG cells. It is also found on the surface of mouse erythrocytes.

  • Alternative names

    • Stage specific embryonic antigen 3 antibody

Images

  • Flow Cytometry - Anti-SSEA3 antibody [MC631] (ab16286)
    Flow Cytometry - Anti-SSEA3 antibody [MC631] (ab16286)This image is courtesy of Prof Chris Denning's lab, University of Nottingham

    Human induced pluripotent stem cells (iPSCs) stained with ab16286 (red fill). In brief, iPSCs were fixed in 4% formaldehyde (methanol-free) for 15 min at 25°C. Cells were then incubated with the antibody (ab16286, 1/50 dilution) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 647 goat anti-rat IgG at 1/2000 dilution for 30 min at 4°C. iPSCs differentiated for 28 days toward a cardiomyocyte lineage (blue fill) were used as a negative control. Acquisition of >30,000 total events were collected using a 100mW solid state diode laser (640nm) and 670/30 bandpass filter.

  • Immunocytochemistry/ Immunofluorescence - Anti-SSEA3 antibody [MC631] (ab16286)
    Immunocytochemistry/ Immunofluorescence - Anti-SSEA3 antibody [MC631] (ab16286)This image is courtesy of the laboratory of Peter Andrews.
    The image shows cell membrane staining of 2102Ep human embryonic carcinoma cells using SSEA3 specific antibody ab16286.
  • Flow Cytometry - Anti-SSEA3 antibody [MC631] (ab16286)
    Flow Cytometry - Anti-SSEA3 antibody [MC631] (ab16286)This image is from an anonymous collaborator.
    Anti-SSEA3 antibody, ab16286 can be used as a marker of Embryonic Carcinoma cells in Flow Cytometry/FACS. The top histogram (A) shows undifferentiated NTERA2 cells (i.e. without retinoic acid) recognised by ab16286. However, upon differentiation (by the addition of retinoic acid 10-5M for 7 days), the antibody lost the ability to recognise these cells as shown in the bottom histogram (B).

References (31)

Publishing research using ab16286? Please let us know so that we can cite the reference in this datasheet.

ab16286 has been referenced in 31 publications.

  • Trevisan M  et al. Generation of a transgene-free induced pluripotent stem cells line (UNIPDi002-A) from oral mucosa epithelial stem cells carrying the R304Q mutation in TP63 gene. Stem Cell Res 28:149-152 (2018). PubMed: 29486400
  • Trevisan M  et al. Induced pluripotent stem cells line (UNIPDi003-A) from a patient affected by EEC syndrome carrying the R279H mutation in TP63 gene. Stem Cell Res 28:141-144 (2018). Human . PubMed: 29477592
  • Ortuño-Costela MDC  et al. Generation of a human iPSC line, IISHDOi002-A, with a 46, XY/47, XYY mosaicism and belonging to an African mitochondrial haplogroup. Stem Cell Res 28:131-135 (2018). ICC/IF ; Human . PubMed: 29471262
  • Alvisi G  et al. Generation of a transgene-free human induced pluripotent stem cell line (UNIPDi001-A) from oral mucosa epithelial stem cells. Stem Cell Res 28:177-180 (2018). ICC/IF ; Human . PubMed: 29547871
  • Luo Y  et al. Generation of an induced pluripotent stem cell line from an adult male with 45,X/46,XY mosaicism. Stem Cell Res 27:42-45 (2018). ICC/IF ; Human . PubMed: 29320756
View all Publications for this product

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1-3 of 3 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-SSEA3 antibody [MC631]

 Average
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human ES cell (hues7))
Specification
human ES cell (hues7)
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
1% serum 0.1% BSA in PBS as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
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Abcam user community

Verified customer

Submitted Jun 08 2012

Flow Cytometry abreview for Anti-SSEA3 antibody [MC631]

 Excellent
Abreviews
abreview image
Application
Flow Cytometry
Sample
Human Cell (human ES cell undifferentiated)
Specification
human ES cell undifferentiated
Preparation
Cell harvesting/tissue preparation method: cell dissociation buffer
Sample buffer: FACS buffer (0.2% BSA, 0.1% sodium azide in PBS)
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
FSC vs SSC on negative control sample
Read More

Abcam user community

Verified customer

Submitted Jun 08 2012

Question

Hello, I would like to let you know that I made a decision to ask you for the anti-cytokeratin 18 antibody (ab 32118) as replacement item. Please, let me know if this is fine with you Thanks a lot

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Answer

Thanks for your reply. I would be happy to send a vial of ab32118. Could you just confirm your shipping address? Thank you, and I look forward to hearing from you.

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