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    ssea4-antibody-mc813-70-ab16287.pdf

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Stem Cells Embryonic Stem Cells Surface Molecules
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Anti-SSEA4 antibody [MC813-70] (ab16287)

  • Datasheet
  • SDS
Reviews (11)Q&A (7)References (147)

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Flow Cytometry - Anti-SSEA4 antibody [MC813-70] (ab16287)
  • Immunocytochemistry/ Immunofluorescence - Anti-SSEA4 antibody [MC813-70] (ab16287)
  • Immunocytochemistry/ Immunofluorescence - Anti-SSEA4 antibody [MC813-70] (ab16287)
  • Immunocytochemistry/ Immunofluorescence - Anti-SSEA4 antibody [MC813-70] (ab16287)
  • Flow Cytometry - Anti-SSEA4 antibody [MC813-70] (ab16287)
  • Immunocytochemistry/ Immunofluorescence - Anti-SSEA4 antibody [MC813-70] (ab16287)
  • Immunocytochemistry/ Immunofluorescence - Anti-SSEA4 antibody [MC813-70] (ab16287)

Key features and details

  • Mouse monoclonal [MC813-70] to SSEA4
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG3

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Overview

  • Product name

    Anti-SSEA4 antibody [MC813-70]
  • Description

    Mouse monoclonal [MC813-70] to SSEA4
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Tissue, cells or virus corresponding to Human SSEA4. Human embryonal carcinoma cell line 2102Ep

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Purification notes

    Tissue culture supernatant was cross flow concentrated.
  • Clonality

    Monoclonal
  • Clone number

    MC813-70
  • Myeloma

    Sp2/0
  • Isotype

    IgG3
  • Light chain type

    kappa
  • Research areas

    • Stem Cells
    • Embryonic Stem Cells
    • Surface Molecules
    • Stem Cells
    • Germline Stem Cells
    • Embryonic Germ Cells
    • Developmental Biology
    • Reproduction
    • Germ cell markers
    • Developmental Biology
    • Embryogenesis
    • Embryonic stem cells
    • Intracellular

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG3, kappa monoclonal [MG3-35] - Isotype Control (ab18394)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab16287 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (8)
Use at an assay dependent concentration.
Flow Cyt (2)
Use at an assay dependent concentration.

ab18392 - Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody.

Notes
ICC/IF
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab18392 - Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody.

Target

  • Relevance

    Glycosphingolipids function as mediators of cell adhesion and are modulators of signal transduction. SSEA-4 (Stage-Specific Embryonic Antigen 4) is a glycolipid expressed early in embryonic development and in pluripotent stem cells. This antibody was first described and named as part of a series of embryonic antigens, defined by monoclonal antibodies isolated in the lab of Prof. Davor Solter (Kannagi, R. et al., 1983, EMBO J. 2:2355). SSEA-4 can be used as a marker of human embryonic stem cells, human embryonic carcinoma cells and human embryonic germ cells. Monoclonal antibodies to this target have been widely used in the characterization of pluripotent stem cells. Mouse pluripotent stem cells are not recognised by anti-SSEA-4 antibodies but do express the antigen upon differentiation.
  • Cellular localization

    Cell Membrane
  • Alternative names

    • Stage specific embryonic antigen 4 antibody

Images

  • Flow Cytometry - Anti-SSEA4 antibody [MC813-70] (ab16287)
    Flow Cytometry - Anti-SSEA4 antibody [MC813-70] (ab16287)This image is courtesy of Professor Chris Denning's lab, University of Nottingham

    Human induced pluripotent stem cells (iPSCs) stained with ab16287 (red fill) with secondary only control (blue fill). In brief, iPSCs were fixed in 4% formaldehyde (methanol-free) for 15 min at 25°C. Cells were then incubated with the antibody (ab16287, 1/9600 dilution) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG at 1/1000 dilution for 30 min at 4°C. iPSCs differentiated for 14 days toward a cardiomyocyte lineage were used as a negative control. Acquisition of >20,000 total events were collected using a 100mW solid state diode laser (488nm) and 529/28 bandpass filter.

  • Immunocytochemistry/ Immunofluorescence - Anti-SSEA4 antibody [MC813-70] (ab16287)
    Immunocytochemistry/ Immunofluorescence - Anti-SSEA4 antibody [MC813-70] (ab16287)This image is courtesy of an Abreview submitted by Sun Jeong Kim

    ab16287 staining SSEA4 in rat tendon derived stem cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.25% Triton X-100 in PBS and blocked with 1% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/100) for 20 hours at 4°C. An Alexa Fluor® 594-conjugated donkey anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-SSEA4 antibody [MC813-70] (ab16287)
    Immunocytochemistry/ Immunofluorescence - Anti-SSEA4 antibody [MC813-70] (ab16287)Image from Ferro F et al., PLoS One. 2012;7(7):e41774. Epub 2012 Jul 23. Fig 6.; doi:10.1371/journal.pone.0041774; July 23, 2012, PLoS ONE 7(7): e41774.
    Immunofluorescence analysis of Human dental pulp stem cells, staining SSEA4 with ab16287 at 1/40 dilution. A FITC-conjugated anti-mouse IgG was used as the secondary antibody.
  • Immunocytochemistry/ Immunofluorescence - Anti-SSEA4 antibody [MC813-70] (ab16287)
    Immunocytochemistry/ Immunofluorescence - Anti-SSEA4 antibody [MC813-70] (ab16287)This image is courtesy of an abreview submitted by Vladimir Milenkovic, University Hospital Regensburg, Germany

    Immunocytochemistry/ Immunofluorescence analysis of human iPSC cells labeling SSEA4 with ab16287 at 1/500 dilution. Cells were fixed with paraformaldehyde and  permeabilized with 0.5% TX100. Cells were blocked with 5% serum for 20 minutes at 25°C. A goat polyclonal anti-mouse Cy3 secondary antibody at 1/500 dilution was used. 

    See Abreview

  • Flow Cytometry - Anti-SSEA4 antibody [MC813-70] (ab16287)
    Flow Cytometry - Anti-SSEA4 antibody [MC813-70] (ab16287)
    anti-SSEA4 antibody, ab16297 can be used as a marker of Embryonic Carcinoma cells in Flow Cytometry/FACS. As can be seen from the histograms, in non-differentiating conditions (i.e. without retinoic acid) NTERA2 cells were recognised by ab16297. However, upon differentiation (addition of retinoic acid), the antibody lost the ability to recognise the cells.
  • Immunocytochemistry/ Immunofluorescence - Anti-SSEA4 antibody [MC813-70] (ab16287)
    Immunocytochemistry/ Immunofluorescence - Anti-SSEA4 antibody [MC813-70] (ab16287)
    2102ep Human Embryonic Carcinoma cells were stained with SSEA4 antibody ab16287. As expected, staining localised to the cell surface (green). Nuclei are stained blue using Hoechst.
  • Immunocytochemistry/ Immunofluorescence - Anti-SSEA4 antibody [MC813-70] (ab16287)
    Immunocytochemistry/ Immunofluorescence - Anti-SSEA4 antibody [MC813-70] (ab16287)This image is a courtesy of Fiona Lewis

    ab16287 staining SSEA4 in human Embryonic Stem Cells, HUES7 by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with Triton and blocking with 10% serum for 1 hour was performed. Samples were incubated with primary antibody (1/100: in 1% serum, 0.1% Triton in PBS) for 1 hour at 37°C. An Alexa Fluor® 588-conjugated goat polyclonal to mouse IgG was used at dilution at 1/100 as secondary antibody.

    See Abreview

Protocols

  • Mouse on Mouse staining protocol

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (147)

Publishing research using ab16287? Please let us know so that we can cite the reference in this datasheet.

ab16287 has been referenced in 147 publications.

  • Zhang Y  et al. Cryopreservation of human induced pluripotent stem cells by using a new CryoLogic vitrification method. Cryobiology 98:210-214 (2021). PubMed: 33147488
  • Lavra L  et al. Generation and characterization of the human induced pluripotent stem cell (hiPSC) line NCUFi001-A from a patient carrying KCNQ1 G314S mutation. Stem Cell Res 54:102418 (2021). PubMed: 34130155
  • Sutcliffe DJ  et al. Induced pluripotent stem cells from subjects with Lesch-Nyhan disease. Sci Rep 11:8523 (2021). PubMed: 33875724
  • Yan R  et al. Generation of a human induced pluripotent stem cell line (SMUSHi001-A) from a patient with 46, XX male sex reversal syndrome carrying the SRY gene. Stem Cell Res 54:102397 (2021). PubMed: 34098200
  • Wu F  et al. Generation of WAe001-A-58 human embryonic stem cell line with inducible expression of the SARS-CoV-2 nucleocapsid protein. Stem Cell Res 53:102197 (2021). PubMed: 33771478
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-10 of 18 Abreviews or Q&A

Flow Cytometry abreview for Anti-SSEA4 antibody [MC813]

Poor
Abreviews
Abreviews
abreview image
Application
Flow Cytometry
Sample
Human Cell (HEK 293)
Permeabilization
No
Gating Strategy
FSC vs SSC on negative control sample
Specification
HEK 293
Preparation
Cell harvesting/tissue preparation method: Trypsin
Sample buffer: 0.1% BSA in PBS -Ca-Mg
Fixation
no fixation
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Bora Baskaner

Verified customer

Submitted Dec 21 2015

Immunocytochemistry/ Immunofluorescence abreview for Anti-SSEA4 antibody [MC813]

Average
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Horse Cell (testis tissues)
Specification
testis tissues
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mr. Heejun Jung

Verified customer

Submitted Aug 19 2015

Immunocytochemistry/ Immunofluorescence abreview for Anti-SSEA4 antibody [MC813]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (iCell® iPSC Diff Day 0)
Permeabilization
No
Specification
iCell® iPSC Diff Day 0
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Acetone
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 11 2015

Immunocytochemistry/ Immunofluorescence abreview for Anti-SSEA4 antibody [MC813] - Embryonic Stem Cell Marker

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Sample
Rat Cell (tendon drived stem cell)
Specification
tendon drived stem cell
Permeabilization
Yes - PBS containing 0.25% Triton X-100
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mrs. Sun Jeong Kim

Verified customer

Submitted Feb 25 2014

Immunocytochemistry/ Immunofluorescence abreview for Anti-SSEA4 antibody [MC813] - Embryonic Stem Cell Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Sample
Human Cell (iPS)
Specification
iPS
Permeabilization
No
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mr. Faisal Alzahrani

Verified customer

Submitted Dec 04 2013

Immunocytochemistry abreview for Anti-SSEA4 antibody [MC813] - Embryonic Stem Cell Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Human Cultured Cells (H9 human)
Specification
H9 human
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 10 2013

Flow Cytometry abreview for Anti-SSEA4 antibody [MC813] - Embryonic Stem Cell Marker

Poor
Abreviews
Abreviews
abreview image
Application
Flow Cytometry
Fixation
Paraformaldehyde
Permeabilization
No
Sample
Human Cell (human pluripotent iPS cells)
Specification
human pluripotent iPS cells
Gating Strategy
FSC vs SSC on negative control sample
Preparation
Cell harvesting/tissue preparation method: cell dissociation buffer
Sample buffer: FACS buffer (0.2% BSA, 0.1% sodium azide in PBS)
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 04 2013

Immunocytochemistry/ Immunofluorescence

Average
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Sample
Human Cultured Cells (induced pluripotent stem cells)
Specification
induced pluripotent stem cells
Permeabilization
No
Fixative
Methanol
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 07 2013

Immunocytochemistry/ Immunofluorescence abreview for Anti-SSEA4 antibody [MC813] - Embryonic Stem Cell Marker

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (undifferentiated human ES cells)
Specification
undifferentiated human ES cells
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
1% serum, 0.1% BSA in PBS as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Sep 04 2012

Question

Inquiry: Dear Sir/Madam I have some questions about your products [anti-CD24 antibody (ab110448), anti-CD44 antibody (ab119863), anti-SSEA4 antibody (ab16287)]. I want to know that these products had been conjugated with fluorophores or dyes for flow cytometric detection or not and they need secondary antibody if it is yes, what are their colors. Thanks a lot. Your Sincerely

Read More

Abcam community

Verified customer

Asked on Nov 21 2012

Answer

Thank you for contacting us.
I can confirm that the anti-CD24 antibody (ab110448), anti-CD44 antibody (ab119863), and anti-SSEA4 antibody (ab16287) are not conjugated to any fluorophores. A secondary antibody needs to be used.
I found in our product catalogue that we offer fluorophor conjugated Antibodies against the CD24 and the CD44. You might want to have a look at on our website. I can confirm that we regrettably not have a fluorophore conjugated antibody against the SSEA4.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More

Abcam Scientific Support

Answered on Nov 21 2012

1-10 of 18 Abreviews or Q&A

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