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Rabbit polyclonal to SSH3
Predicted to work with:
Chimpanzee, Gorilla, Orangutan
Synthetic peptide corresponding to a region between residues 609 and 659 of human SSH3 (NP_060327.2)
HeLa and 293T whole cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
ab76945 was affinity purified using an epitope specific to SSH3 immobilized on solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 73 kDa.
Use at 2-5 µg/mg of lysate.
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protein phosphatase which may play a role in the regulation of actin filament dynamics. Can dephosphorylate and activate the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly.
Belongs to the protein-tyrosine phosphatase family.
Contains 1 tyrosine-protein phosphatase domain.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cytoplasm > cytoskeleton. Nucleus.
Information by UniProt
hSSH 3L antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SSH3 antibody (ab76945)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling SSH3 with ab76945 at 1/200 (1µg/ml). Detection: DAB.
Western blot - Anti-SSH3 antibody (ab76945)
All lanes :
Anti-SSH3 antibody (ab76945) at 0.04 µg/ml
Lane 1 :
HeLa whole cell lysate at 50 µg
Lane 2 :
HeLa whole cell lysate at 15 µg
Lane 3 :
HeLa whole cell lysate at 5 µg
Lane 4 :
293T whole cell lysate at 50 µg
Predicted band size:
Observed band size:
80 kDa (
why is the actual band size different from the predicted?
Additional bands at:
51 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation - Anti-SSH3 antibody (ab76945)
ab76945 at 1µg/ml staining SSH3 in HeLa whole cell lysate immunoprecipitated using ab76945 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"