Overview

  • Product name

    Anti-SSPN antibody [EPR15515]
    See all SSPN primary antibodies
  • Description

    Rabbit monoclonal [EPR15515] to SSPN
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SSPN aa 150 to the C-terminus. The exact sequence is proprietary.
    Database link: Q14714

  • Positive control

    • Human fetal heart, skeletal muscle, A673 and 293 lysates; Mouse heart, rat brain, rat heart tissue lysates; NIH 3T3 cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab186730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).

Target

  • Relevance

    The SSPN gene is expressed in a variety of tissues with highest levels in muscle, where alternative splice variants have been observed. In certain tumors KRAS2, SSPN, and ITPR2 are co-amplified. The function of this gene is unknown.
  • Cellular localization

    Cell membrane, sarcolemma; Multi-pass membrane protein. Note: Also found in myotendinous junctions and in the postsynaptic membrane of neuromuscular junctions.
  • Database links

  • Alternative names

    • K ras oncogene associated gene antibody
    • K ras oncogene associated protein antibody
    • Kirsten ras associated protein antibody
    • KRAG antibody
    • Sarcospan antibody
    • SPN1 antibody
    • SPN2 antibody
    see all

Images

  • All lanes : Anti-SSPN antibody [EPR15515] (ab186730) at 1/10000 dilution

    Lane 1 : Human fetal heart tissue lysate
    Lane 2 : Human skeletal muscle tissue lysate
    Lane 3 : A673 cell lysate
    Lane 4 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 27 kDa

  • All lanes : Anti-SSPN antibody [EPR15515] (ab186730) at 1/1000 dilution

    Lane 1 : Mouse heart tissue lysate
    Lane 2 : Rat brain tissue lysate
    Lane 3 : Rat heart tissue lysate
    Lane 4 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size: 27 kDa

References

ab186730 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab186730.
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