Anti-SSR3 antibody (ab121458)
Key features and details
- Rabbit polyclonal to SSR3
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-SSR3 antibody
See all SSR3 primary antibodies -
Description
Rabbit polyclonal to SSR3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
antigen sequence, corresponding to amino acids 83-133 (VLKHKVAQKR EDAVSKEVTR KLSEADNRKM SRKEKDERIL WKKNEVADYE A) of Human SSR3.
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Positive control
- IHC-P: Human colon and cerebral cortex tissues. Mouse brain and choroid plexus tissues. WB: RPMI-8226, NIH/3T3, and NBT-II cell lysates, Human cerebral cortex and mouse cerebellum tissue lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121458 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins. -
Sequence similarities
Belongs to the TRAP-gamma family. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 6747 Human
- Entrez Gene: 67437 Mouse
- Entrez Gene: 81784 Rat
- Omim: 606213 Human
- SwissProt: Q9UNL2 Human
- SwissProt: Q4FK79 Mouse
- SwissProt: Q08013 Rat
- Unigene: 518346 Human
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Alternative names
- Signal sequence receptor gamma antibody
- Signal sequence receptor subunit gamma antibody
- SSR-gamma antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse brain tissue labelling SSR3 with ab121458 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Anti-SSR3 antibody (ab121458) at 0.4 µg/ml + Human cerebral cortex tissue lysate
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling SSR3 with ab121458 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse choroid plexus tissue labelling SSR3 with ab121458 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebral cortex tissue labelling SSR3 with ab121458 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Anti-SSR3 antibody (ab121458) at 0.4 µg/ml + Mouse cerebellum tissue lysate
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All lanes : Anti-SSR3 antibody (ab121458) at 0.4 µg/ml
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lane 2 : NBT-II (rat urinary bladder tumor cell line) cell lysate -
Anti-SSR3 antibody (ab121458) at 0.4 µg/ml + RPMI-8226 (human lymphoblast cell line) cell lysate
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab121458 has been referenced in 1 publication.
- Huang S et al. Overexpression of signal sequence receptor ? (SSR3) predicts poor survival in patients with hepatocellular carcinoma. Hum Pathol N/A:N/A (2018). PubMed: 29940286