Overview

  • Product name

    Anti-SSR3 antibody - N-terminal
    See all SSR3 primary antibodies
  • Description

    Rabbit polyclonal to SSR3 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human SSR3 aa 8-23 (N terminal).
    Sequence:

    KQQSEEDLLLQDFSRN


    Database link: Q9UNL2

  • Positive control

    • WB: Rat liver, rat spleen, A431, HeLa, U87 and SMMC lysates; IHC-P: Human prostatic cancer, Rat brain and intestine tissues, and Mouse intestine tissue. ICC: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab190936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC Use a concentration of 0.5 - 1 µg/ml.
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 21 kDa.

Target

Images

  • SSR3 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit ab190936 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.

  • Immunohistochemical analysis of paraffin-embedded Rat brain tissue labeling SSR3 with ab190936 at 1 µg/ml.

  • SSR3 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab190936 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.

  • SSR3 was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab190936 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.

  • SSR3 was detected in immunocytochemical section of HeLa cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml ab190936 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.

  • All lanes : Anti-SSR3 antibody - N-terminal (ab190936) at 0.5 µg/ml

    Lane 1 : Rat liver tissue lysate
    Lane 2 : Rat spleen tissue lysate
    Lane 3 : A431 cell lysate
    Lane 4 : HeLa cell lysate
    Lane 5 : U87 cell lysate
    Lane 6 : SMMC cell lysate

    Predicted band size: 21 kDa

  • Immunohistochemical analysis of paraffin-embedded Human prostatic cancer tissue labeling SSR3 with ab190936 at 1 µg/ml.

  • Immunohistochemical analysis of paraffin-embedded Rat intestine tissue labeling SSR3 with ab190936 at 1 µg/ml.

References

ab190936 has not yet been referenced specifically in any publications.

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