Anti-SSRP1 antibody [10D7] (ab26212)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-SSRP1 antibody [10D7]
See all SSRP1 primary antibodies -
DescriptionMouse monoclonal [10D7] to SSRP1
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Host speciesMouse
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Specificityab26212 recognises Structure specific recognition protein 1 (SSRP1).
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Tested applicationsSuitable for: ICC/IF, IP, WB, Flow Cytmore details
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Species reactivityReacts with: Human
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Immunogen
Recombinant SSRP1 fragment (Human)
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Positive control
- HeLa cell lysate
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 50% Glycerol, PBS, pH 7.2 -
Concentration information loading... -
PurityProtein G purified
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ClonalityMonoclonal
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Clone number10D7
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IsotypeIgG2b
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Light chain typekappa
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Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab26212 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 10 µg/ml. | |
| IP | 1/500. (see Abreview) | |
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 81 kDa. | |
| Flow Cyt | Use 1µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
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Target
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FunctionComponent of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Binds specifically to double-stranded DNA and at low levels to DNA modified by the antitumor agent cisplatin. May potentiate cisplatin-induced cell death by blocking replication and repair of modified DNA. Also acts as a transcriptional coactivator for p63/TP63.
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Sequence similaritiesBelongs to the SSRP1 family.
Contains 1 HMG box DNA-binding domain. -
DomainThe HMG box DNA-binding domain mediates DNA-binding. It has both affinity and specificity for DNA damaged globally with cisplatin.
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Post-translational
modificationsPhosphorylated by CK2 following UV but not gamma irradiation. Phosphorylation inhibits its DNA-binding activity.
Ubiquitinated. Polyubiquitinated following caspase cleavage resulting in degradation of the N-terminal ubiquitinated part of the cleaved protein.
Sumoylated. -
Cellular localizationNucleus. Chromosome. Colocalizes with RNA polymerase II on chromatin. Recruited to actively transcribed loci.
- Information by UniProt
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Database links
- Entrez Gene: 100413716 Human
- Entrez Gene: 6749 Human
- Omim: 604328 Human
- SwissProt: Q08945 Human
- Unigene: 523680 Human
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Alternative names
- Chromatin-specific transcription elongation factor 80 kDa subunit antibody
- Cisplatin DNA SSRP antibody
- Facilitates chromatin remodeling 80 kDa subunit antibody
see all
Images
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![Western blot - Anti-SSRP1 antibody [10D7] (ab26212)](https://www.abcam.com/ps/products/26/ab26212/Images/ab26212.png)
Western blot - Anti-SSRP1 antibody [10D7] (ab26212)Anti-SSRP1 antibody [10D7] (ab26212) at 1 µg/ml + HeLa cell lysate
Secondary
Goat anti-mouse Ig conjugated to HRP.
Developed using the ECL technique.
Predicted band size: 81 kDa
Observed band size: 81 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-SSRP1 antibody [10D7] (ab26212)](https://www.abcam.com/ps/products/26/ab26212/Images/SSRP1-Primary-antibodies-ab26212-6.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SSRP1 antibody [10D7] (ab26212)ICC/IF image of ab26212 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26212, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
![Flow Cytometry - Anti-SSRP1 antibody [10D7] (ab26212)](https://www.abcam.com/ps/products/26/ab26212/Images/SSRP1-Primary-antibodies-ab26212-7.jpg)
Flow Cytometry - Anti-SSRP1 antibody [10D7] (ab26212)Overlay histogram showing HeLa cells stained with ab26212 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26212, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Young LM et al. TIMELESS Forms a Complex with PARP1 Distinct from Its Complex with TIPIN and Plays a Role in the DNA Damage Response. Cell Rep 13:451-459 (2015). Read more (PubMed: 26456830) »
