Anti-SSRP1 antibody [10D7] (ab26212)
![Western blot - Anti-SSRP1 antibody [10D7] (ab26212)](https://www.abcam.com/ps/products/26/ab26212/Images/ab26212-36889-anti-ssrp1-antibody-10d7-western-blot.png "Western blot - Anti-SSRP1 antibody [10D7] (ab26212)")
Key features and details
- Mouse monoclonal [10D7] to SSRP1
- Suitable for: ICC/IF, WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-SSRP1 antibody [10D7]
See all SSRP1 primary antibodies -
Description
Mouse monoclonal [10D7] to SSRP1 -
Host species
Mouse -
Specificity
ab26212 recognises Structure specific recognition protein 1 (SSRP1). -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanWB Human
-
Immunogen
Recombinant fragment corresponding to Human SSRP1.
-
Positive control
- HeLa cell lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
10D7 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab26212 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Guaranteed
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted
Predicted to work for this combination of applications and species but not guaranteed.
Incompatible
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Human
|
| ICC/IF |
Human
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (1) |
Use a concentration of 10 µg/ml.
|
| WB | (4) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 81 kDa.
|
| Flow Cyt |
Use 1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|
| Notes |
|---|
|
ICC/IF
Use a concentration of 10 µg/ml. |
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 81 kDa. |
|
Flow Cyt
Use 1µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|
Target
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Function
Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Binds specifically to double-stranded DNA and at low levels to DNA modified by the antitumor agent cisplatin. May potentiate cisplatin-induced cell death by blocking replication and repair of modified DNA. Also acts as a transcriptional coactivator for p63/TP63. -
Sequence similarities
Belongs to the SSRP1 family.
Contains 1 HMG box DNA-binding domain. -
Domain
The HMG box DNA-binding domain mediates DNA-binding. It has both affinity and specificity for DNA damaged globally with cisplatin. -
Post-translational
modificationsPhosphorylated by CK2 following UV but not gamma irradiation. Phosphorylation inhibits its DNA-binding activity.
Ubiquitinated. Polyubiquitinated following caspase cleavage resulting in degradation of the N-terminal ubiquitinated part of the cleaved protein.
Sumoylated. -
Cellular localization
Nucleus. Chromosome. Colocalizes with RNA polymerase II on chromatin. Recruited to actively transcribed loci. - Information by UniProt
-
Database links
- Entrez Gene: 100413716 Human
- Entrez Gene: 6749 Human
- Omim: 604328 Human
- SwissProt: Q08945 Human
- Unigene: 523680 Human
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Alternative names
- Chromatin-specific transcription elongation factor 80 kDa subunit antibody
- Cisplatin DNA SSRP antibody
- Facilitates chromatin remodeling 80 kDa subunit antibody
see all
Images
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Western blot - Anti-SSRP1 antibody [10D7] (ab26212)Anti-SSRP1 antibody [10D7] (ab26212) at 1 µg/ml + HeLa cell lysate
Secondary
Goat anti-mouse Ig conjugated to HRP.
Developed using the ECL technique.
Predicted band size: 81 kDa
Observed band size: 81 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-SSRP1 antibody [10D7] (ab26212)](https://www.abcam.com/ps/products/26/ab26212/Images/ab26212-36888-anti-ssrp1-antibody-10d7-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SSRP1 antibody [10D7] (ab26212)ICC/IF image of ab26212 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26212, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
![Flow Cytometry - Anti-SSRP1 antibody [10D7] (ab26212)](https://www.abcam.com/ps/products/26/ab26212/Images/ab26212-36887-anti-ssrp1-antibody-10d7-flow-cytometry.jpg)
Flow Cytometry - Anti-SSRP1 antibody [10D7] (ab26212)Overlay histogram showing HeLa cells stained with ab26212 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26212, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
Protocols
References (5)
ab26212 has been referenced in 5 publications.
- LeRoy G et al. LEDGF and HDGF2 relieve the nucleosome-induced barrier to transcription in differentiated cells. Sci Adv 5:eaay3068 (2019). PubMed: 31616795
- Young LM et al. TIMELESS Forms a Complex with PARP1 Distinct from Its Complex with TIPIN and Plays a Role in the DNA Damage Response. Cell Rep 13:451-459 (2015). PubMed: 26456830
- Liberman R et al. Structural analysis of the N-terminal domain of subunit a of the yeast vacuolar ATPase (V-ATPase) using accessibility of single cysteine substitutions to chemical modification. J Biol Chem 288:22798-808 (2013). PubMed: 23740254
- Dinant C et al. Enhanced chromatin dynamics by FACT promotes transcriptional restart after UV-induced DNA damage. Mol Cell 51:469-79 (2013). PubMed: 23973375
- Al-Zeer MA et al. IFN-gamma-inducible Irga6 mediates host resistance against Chlamydia trachomatis via autophagy. PLoS One 4:e4588 (2009). PubMed: 19242543
