Anti-SSRP1 antibody [10D7] (ab26212)

Reviews (6)Q&A (3)References (2)

Overview

  • Product name

    Anti-SSRP1 antibody [10D7]
    See all SSRP1 primary antibodies

  • Description

    Mouse monoclonal [10D7] to SSRP1

  • Host species

    Mouse

  • Specificity

    ab26212 recognises Structure specific recognition protein 1 (SSRP1).

  • Tested applications

    Suitable for: ICC/IF, IP, WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment corresponding to Human SSRP1.

  • Positive control

    • HeLa cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab26212 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
IP 1/500. (see Abreview)
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 81 kDa.
Flow Cyt Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Binds specifically to double-stranded DNA and at low levels to DNA modified by the antitumor agent cisplatin. May potentiate cisplatin-induced cell death by blocking replication and repair of modified DNA. Also acts as a transcriptional coactivator for p63/TP63.

  • Sequence similarities

    Belongs to the SSRP1 family.
    Contains 1 HMG box DNA-binding domain.

  • Domain

    The HMG box DNA-binding domain mediates DNA-binding. It has both affinity and specificity for DNA damaged globally with cisplatin.

  • Post-translational
    modifications

    Phosphorylated by CK2 following UV but not gamma irradiation. Phosphorylation inhibits its DNA-binding activity.
    Ubiquitinated. Polyubiquitinated following caspase cleavage resulting in degradation of the N-terminal ubiquitinated part of the cleaved protein.
    Sumoylated.

  • Cellular localization

    Nucleus. Chromosome. Colocalizes with RNA polymerase II on chromatin. Recruited to actively transcribed loci.
  • Information by UniProt

  • Database links

    • Alternative names

      • Chromatin-specific transcription elongation factor 80 kDa subunit antibody
      • Cisplatin DNA SSRP antibody
      • Facilitates chromatin remodeling 80 kDa subunit antibody
      • Facilitates chromatin transcription complex 80 kDa subunit antibody
      • Facilitates chromatin transcription complex subunit SSRP1 antibody
      • FACT 80 kDa subunit antibody
      • FACT complex subunit SSRP1 antibody
      • FACT80 antibody
      • FACTp80 antibody
      • hSSRP1 antibody
      • Recombination signal sequence recognition protein 1 antibody
      • Recombination signal sequence recognition protein antibody
      • SSRP 1 antibody
      • SSRP1 antibody
      • SSRP1_HUMAN antibody
      • Structure specific recognition protein 1 antibody
      • Structure-specific recognition protein 1 antibody
      • T160 antibody
      see all

    Images

    • Western blot - Anti-SSRP1 antibody [10D7] (ab26212)
      Western blot - Anti-SSRP1 antibody [10D7] (ab26212)
      Anti-SSRP1 antibody [10D7] (ab26212) at 1 µg/ml + HeLa cell lysate

      Secondary
      Goat anti-mouse Ig conjugated to HRP.

      Developed using the ECL technique.

      Predicted band size: 81 kDa
      Observed band size: 81 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-SSRP1 antibody [10D7] (ab26212)
      Immunocytochemistry/ Immunofluorescence - Anti-SSRP1 antibody [10D7] (ab26212)
      ICC/IF image of ab26212 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26212, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Flow Cytometry - Anti-SSRP1 antibody [10D7] (ab26212)
      Flow Cytometry - Anti-SSRP1 antibody [10D7] (ab26212)
      Overlay histogram showing HeLa cells stained with ab26212 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26212, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

    References

    This product has been referenced in:

    • Young LM  et al. TIMELESS Forms a Complex with PARP1 Distinct from Its Complex with TIPIN and Plays a Role in the DNA Damage Response. Cell Rep 13:451-459 (2015). Read more (PubMed: 26456830) »
    • Al-Zeer MA  et al. IFN-gamma-inducible Irga6 mediates host resistance against Chlamydia trachomatis via autophagy. PLoS One 4:e4588 (2009). Read more (PubMed: 19242543) »
    See all 2 Publications for this product

    Publishing research using ab26212? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-9 of 9 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-SSRP1 antibody [10D7]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HEK293)
    Permeabilization
    Yes - 0.3% Triton X-100 in blocking buffer
    Specification
    HEK293
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 14 2018

    Western blot abreview for Anti-SSRP1 antibody [10D7]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Lymphocytes)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Loading amount
    30 µg
    Specification
    Lymphocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 25 2017

    Western blot abreview for Anti-SSRP1 antibody [10D7]

     Inconclusive
    Abreviews
    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing (10%)
    Sample
    Xenopus laevis Cell lysate - whole cell (Egg)
    Specification
    Egg
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 18 2014

    Western blot abreview for Anti-SSRP1 antibody [10D7]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    0.25 µg
    Gel Running Conditions
    Non-reduced Denaturing (12% SDS-PAGE)
    Sample
    Human Recombinant protein (Baculovirus expression)
    Specification
    Baculovirus expression
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 30 2013

    Question

    Hi, I am interested in this antibody (ab26212; mouse monoclonal SSRP1).  However, can I check if this antibody can recognize mouse SSRP1 protein?  There was no information on the sequence of the immunogen.  Many thanks.

    Read More
    Answer

    Thank you very much for your interest in ab26212. To our knowledge, ab26212 has not been tested in mouse. Therefore, I can offer a discount off a future purchase if you buy ab26212 now, test it in mouse and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab26212  in mouse. I will then send a discount code. This code must be issued before purchasing ab26212 so please wait for my reply before ordering. 2. Purchase ab26212 either by phone, fax, or online (www.abcam.com). 3. Test it in mouse. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab26212 turns out to be unsuitable for mouse, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.  

    Read More

    Question

    Sorry I just saw the answer to my question on sequence homology at the end of your email. Yes, I will like to proceed, and will test it on mouse sample. Thanks.

    Read More
    Answer

    DISCOUNT CODE: XXXXXXXXX Expiration date: [06-March-2012] I am very pleased to hear you would like to accept our offer and test ab26212 in [Mouse]. This code will give you 1 free [PRIMARY ANTIBODY] before the expiration date. To redeem this offer, please submit an Abreview for [Mouse] and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Question

     Hi, I am interested in this antibody (ab26212 mouse monoclonal SSRP1). However, can I check if this antibody can recognize mouse SSRP1 protein? There was no information on the sequence of the immunogen. Many thanks. Regards, Wee Wei

    Read More
    Answer

    Thank you very much for your interest in ab26212. We do not currently have an image of ab26212 in mouse. Therefore, I can offer a discount off a future purchase if you buy ab26212 now, use it mouse and submit an image of your results to us in the form of an Abreview. The discount would be to the value of 1 free PRIMARY ANTIBODY. It doesn’t matter whether the result is positive or negative, we would just really like to receive your feedback. Of course, if the results are negative you will be covered by our Abpromise guarantee and eligible for a refund or replacement. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and send an image of ab26212 in mouse. I will then send a discount code. This code must be issued before purchasing ab26212 so please wait for my reply before ordering. 2. Purchase ab2612 either by phone, fax, or online (www.abcam.com). 3. Use it in mouse. 4. Send us an image of the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount. PS: The mouse and human SSRP1 sequences are almost 97% similar so this antibody will most likely cross react.

    Read More

    Immunoprecipitation abreview for Anti-SSRP1 antibody [10D7]

     Excellent
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (HEK293 cells)
    Total protein in input
    1e+006 cells
    Specification
    HEK293 cells
    Immuno-precipitation step
    Protein G
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 18 2010

    Western blot abreview for Anti-SSRP1 antibody [10D7]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (mES cells)
    Loading amount
    1 µg
    Specification
    mES cells
    Gel Running Conditions
    Non-reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 01 2010

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