• Product name

    Anti-SSRP1 antibody [3E4]
    See all SSRP1 primary antibodies
  • Description

    Mouse monoclonal [3E4] to SSRP1
  • Host species

  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment (Human)

  • Positive control

    • HeLa cell lysate



Our Abpromise guarantee covers the use of ab26229 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 81 kDa.
IP 1/200.


  • Function

    Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Binds specifically to double-stranded DNA and at low levels to DNA modified by the antitumor agent cisplatin. May potentiate cisplatin-induced cell death by blocking replication and repair of modified DNA. Also acts as a transcriptional coactivator for p63/TP63.
  • Sequence similarities

    Belongs to the SSRP1 family.
    Contains 1 HMG box DNA-binding domain.
  • Domain

    The HMG box DNA-binding domain mediates DNA-binding. It has both affinity and specificity for DNA damaged globally with cisplatin.
  • Post-translational

    Phosphorylated by CK2 following UV but not gamma irradiation. Phosphorylation inhibits its DNA-binding activity.
    Ubiquitinated. Polyubiquitinated following caspase cleavage resulting in degradation of the N-terminal ubiquitinated part of the cleaved protein.
  • Cellular localization

    Nucleus. Chromosome. Colocalizes with RNA polymerase II on chromatin. Recruited to actively transcribed loci.
  • Information by UniProt
  • Database links

  • Alternative names

    • Chromatin-specific transcription elongation factor 80 kDa subunit antibody
    • Cisplatin DNA SSRP antibody
    • Facilitates chromatin remodeling 80 kDa subunit antibody
    • Facilitates chromatin transcription complex 80 kDa subunit antibody
    • Facilitates chromatin transcription complex subunit SSRP1 antibody
    • FACT 80 kDa subunit antibody
    • FACT complex subunit SSRP1 antibody
    • FACT80 antibody
    • FACTp80 antibody
    • hSSRP1 antibody
    • Recombination signal sequence recognition protein 1 antibody
    • Recombination signal sequence recognition protein antibody
    • SSRP 1 antibody
    • SSRP1 antibody
    • SSRP1_HUMAN antibody
    • Structure specific recognition protein 1 antibody
    • Structure-specific recognition protein 1 antibody
    • T160 antibody
    see all


  • Anti-SSRP1 antibody [3E4] (ab26229) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7898)

    Developed using the ECL technique.

    Predicted band size: 81 kDa

  • 2.5 mcg of SSRP1 were immobilized on 50 mcL of anti-mouse Dynabeads prior to IP. Nuclear lysate from HeLa cells treated with Benzonase was loaded at 1 x 107 cells. ab26229 used at a 1/200 dilution for IP. After IP the beads were washed three times with TBS + 0.5%NP40 + 5mM EDTA. ab26229 used at 1/1000 dilution for WB.

    See Abreview


This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

Human Cell lysate - nuclear (HeLa nuclei treated with benzonase)
Total protein in input
1e+007 cells
HeLa nuclei treated with benzonase
Immuno-precipitation step
Other - 2.5 mcg of SSRP1 were immobilized on 50 mcL of anti-mouse Dynabeads (Invitrogen) prior to IP

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Submitted Feb 06 2012

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