The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 81 kDa.
Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Binds specifically to double-stranded DNA and at low levels to DNA modified by the antitumor agent cisplatin. May potentiate cisplatin-induced cell death by blocking replication and repair of modified DNA. Also acts as a transcriptional coactivator for p63/TP63.
Belongs to the SSRP1 family. Contains 1 HMG box DNA-binding domain.
The HMG box DNA-binding domain mediates DNA-binding. It has both affinity and specificity for DNA damaged globally with cisplatin.
Phosphorylated by CK2 following UV but not gamma irradiation. Phosphorylation inhibits its DNA-binding activity. Ubiquitinated. Polyubiquitinated following caspase cleavage resulting in degradation of the N-terminal ubiquitinated part of the cleaved protein. Sumoylated.
Nucleus. Chromosome. Colocalizes with RNA polymerase II on chromatin. Recruited to actively transcribed loci.
Recombination signal sequence recognition protein 1 antibody
Recombination signal sequence recognition protein antibody
SSRP 1 antibody
Structure specific recognition protein 1 antibody
Structure-specific recognition protein 1 antibody
Western blot - Anti-SSRP1 antibody [3E4] (ab26229)
Anti-SSRP1 antibody [3E4] (ab26229) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7898)
Developed using the ECL technique.
Predicted band size: 81 kDa
Immunoprecipitation - Anti-SSRP1 antibody [3E4] (ab26229)Image courtesy of an anonymous Abreview.
2.5 mcg of SSRP1 were immobilized on 50 mcL of anti-mouse Dynabeads prior to IP. Nuclear lysate from HeLa cells treated with Benzonase was loaded at 1 x 107 cells. ab26229 used at a 1/200 dilution for IP. After IP the beads were washed three times with TBS + 0.5%NP40 + 5mM EDTA. ab26229 used at 1/1000 dilution for WB.