Product nameAnti-SSRP1 antibody
See all SSRP1 primary antibodies
DescriptionRabbit polyclonal to SSRP1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Cow, Dog, Pig, Chimpanzee, Gorilla, Common marmoset, Orangutan
- HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab176848 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 81 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionComponent of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Binds specifically to double-stranded DNA and at low levels to DNA modified by the antitumor agent cisplatin. May potentiate cisplatin-induced cell death by blocking replication and repair of modified DNA. Also acts as a transcriptional coactivator for p63/TP63.
Sequence similaritiesBelongs to the SSRP1 family.
Contains 1 HMG box DNA-binding domain.
DomainThe HMG box DNA-binding domain mediates DNA-binding. It has both affinity and specificity for DNA damaged globally with cisplatin.
modificationsPhosphorylated by CK2 following UV but not gamma irradiation. Phosphorylation inhibits its DNA-binding activity.
Ubiquitinated. Polyubiquitinated following caspase cleavage resulting in degradation of the N-terminal ubiquitinated part of the cleaved protein.
Cellular localizationNucleus. Chromosome. Colocalizes with RNA polymerase II on chromatin. Recruited to actively transcribed loci.
- Information by UniProt
- Chromatin-specific transcription elongation factor 80 kDa subunit antibody
- Cisplatin DNA SSRP antibody
- Facilitates chromatin remodeling 80 kDa subunit antibody
Detection of SSRP1 by Western Blot of Immunoprecipitate.
ab176848 at 0.4µg/ml labeling SSRP1 in HeLa whole cell lysate immunoprecipitated using ab176848 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
All lanes : Anti-SSRP1 antibody (ab176848) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate. at 50 µg
Lane 2 : HeLa whole cell lysate. at 15 µg
Lane 3 : HeLa whole cell lysate. at 5 µg
Developed using the ECL technique.
Predicted band size: 81 kDa
Exposure time: 3 minutes
ab176848 has not yet been referenced specifically in any publications.