Overview

  • Product name

    Anti-SSRP1 antibody [EPR7895]
    See all SSRP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR7895] to SSRP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human SSRP1 (UniProt ID: Q08945)

  • Positive control

    • K562, 293T, MOLT4, C6, RAW 264.7, PC12 or NIH 3T3 cell lysates; Hela cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab137034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 81 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Binds specifically to double-stranded DNA and at low levels to DNA modified by the antitumor agent cisplatin. May potentiate cisplatin-induced cell death by blocking replication and repair of modified DNA. Also acts as a transcriptional coactivator for p63/TP63.
    • Sequence similarities

      Belongs to the SSRP1 family.
      Contains 1 HMG box DNA-binding domain.
    • Domain

      The HMG box DNA-binding domain mediates DNA-binding. It has both affinity and specificity for DNA damaged globally with cisplatin.
    • Post-translational
      modifications

      Phosphorylated by CK2 following UV but not gamma irradiation. Phosphorylation inhibits its DNA-binding activity.
      Ubiquitinated. Polyubiquitinated following caspase cleavage resulting in degradation of the N-terminal ubiquitinated part of the cleaved protein.
      Sumoylated.
    • Cellular localization

      Nucleus. Chromosome. Colocalizes with RNA polymerase II on chromatin. Recruited to actively transcribed loci.
    • Information by UniProt
    • Database links

    • Alternative names

      • Chromatin-specific transcription elongation factor 80 kDa subunit antibody
      • Cisplatin DNA SSRP antibody
      • Facilitates chromatin remodeling 80 kDa subunit antibody
      • Facilitates chromatin transcription complex 80 kDa subunit antibody
      • Facilitates chromatin transcription complex subunit SSRP1 antibody
      • FACT 80 kDa subunit antibody
      • FACT complex subunit SSRP1 antibody
      • FACT80 antibody
      • FACTp80 antibody
      • hSSRP1 antibody
      • Recombination signal sequence recognition protein 1 antibody
      • Recombination signal sequence recognition protein antibody
      • SSRP 1 antibody
      • SSRP1 antibody
      • SSRP1_HUMAN antibody
      • Structure specific recognition protein 1 antibody
      • Structure-specific recognition protein 1 antibody
      • T160 antibody
      see all

    Images

    • All lanes : Anti-SSRP1 antibody [EPR7895] (ab137034) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : MOLT4 cell lysate
      Lane 5 : C6 cell lysate
      Lane 6 : RAW 264.7 cell lysate
      Lane 7 : PC12 cell lysate
      Lane 8 : NIH 3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP conjugated Goat anti Rabbit IgG at 1/2000 dilution

      Predicted band size: 81 kDa

    References

    This product has been referenced in:

    See 1 Publication for this product

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