Recombinant
RabMAb

Recombinant Anti-SSRP1 antibody [EPR7895] - BSA and Azide free (ab240097)

Overview

  • Product name

    Anti-SSRP1 antibody [EPR7895] - BSA and Azide free
    See all SSRP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR7895] to SSRP1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SSRP1 aa 650-750. The exact sequence is proprietary.

  • General notes

    Ab240097 is the carrier-free version of ab137034. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240097 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab240097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 81 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Binds specifically to double-stranded DNA and at low levels to DNA modified by the antitumor agent cisplatin. May potentiate cisplatin-induced cell death by blocking replication and repair of modified DNA. Also acts as a transcriptional coactivator for p63/TP63.
    • Sequence similarities

      Belongs to the SSRP1 family.
      Contains 1 HMG box DNA-binding domain.
    • Domain

      The HMG box DNA-binding domain mediates DNA-binding. It has both affinity and specificity for DNA damaged globally with cisplatin.
    • Post-translational
      modifications

      Phosphorylated by CK2 following UV but not gamma irradiation. Phosphorylation inhibits its DNA-binding activity.
      Ubiquitinated. Polyubiquitinated following caspase cleavage resulting in degradation of the N-terminal ubiquitinated part of the cleaved protein.
      Sumoylated.
    • Cellular localization

      Nucleus. Chromosome. Colocalizes with RNA polymerase II on chromatin. Recruited to actively transcribed loci.
    • Information by UniProt
    • Database links

    • Alternative names

      • Chromatin-specific transcription elongation factor 80 kDa subunit antibody
      • Cisplatin DNA SSRP antibody
      • Facilitates chromatin remodeling 80 kDa subunit antibody
      • Facilitates chromatin transcription complex 80 kDa subunit antibody
      • Facilitates chromatin transcription complex subunit SSRP1 antibody
      • FACT 80 kDa subunit antibody
      • FACT complex subunit SSRP1 antibody
      • FACT80 antibody
      • FACTp80 antibody
      • hSSRP1 antibody
      • Recombination signal sequence recognition protein 1 antibody
      • Recombination signal sequence recognition protein antibody
      • SSRP 1 antibody
      • SSRP1 antibody
      • SSRP1_HUMAN antibody
      • Structure specific recognition protein 1 antibody
      • Structure-specific recognition protein 1 antibody
      • T160 antibody
      see all

    Images

    • Immunofluorescence analysis of Hela cells labelling SSRP1 with ab137034 at 1/250 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137034).

    References

    ab240097 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab240097.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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