Key features and details
- Rabbit polyclonal to SSRP1 - N-terminal
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SSRP1 antibody - N-terminal
See all SSRP1 primary antibodies
DescriptionRabbit polyclonal to SSRP1 - N-terminal
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Recombinant fragment (GST-tag) corresponding to Human SSRP1 (N terminal).
Database link: Q08945
- WB: HeLa nuclear extracts.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservatives: 0.05% Sodium azide, 0.05% Proclin 300
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab231596 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 81 kDa.|
FunctionComponent of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Binds specifically to double-stranded DNA and at low levels to DNA modified by the antitumor agent cisplatin. May potentiate cisplatin-induced cell death by blocking replication and repair of modified DNA. Also acts as a transcriptional coactivator for p63/TP63.
Sequence similaritiesBelongs to the SSRP1 family.
Contains 1 HMG box DNA-binding domain.
DomainThe HMG box DNA-binding domain mediates DNA-binding. It has both affinity and specificity for DNA damaged globally with cisplatin.
modificationsPhosphorylated by CK2 following UV but not gamma irradiation. Phosphorylation inhibits its DNA-binding activity.
Ubiquitinated. Polyubiquitinated following caspase cleavage resulting in degradation of the N-terminal ubiquitinated part of the cleaved protein.
Cellular localizationNucleus. Chromosome. Colocalizes with RNA polymerase II on chromatin. Recruited to actively transcribed loci.
- Information by UniProt
- Chromatin-specific transcription elongation factor 80 kDa subunit antibody
- Cisplatin DNA SSRP antibody
- Facilitates chromatin remodeling 80 kDa subunit antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231596 has not yet been referenced specifically in any publications.